BBa_K091164
type: ComponentDefinition
persistent identity: BBa K091164
display id: BBa_K091164
version: 1
was generated by: iGEM to SBOL conversion
description: pLux/cI-RBS-LuxS-RBS-Mnt-TT
created: 2008-07-27T11:00:00Z
modified: 2015-05-08T01:08:38Z
mutable provenance: registry
owned by: james
owned by: myers
mutable description: This construct is a modified promoter attched to sequences for an activator and repressor. The activator is LuxS, the AI-2 synthase. Both ribosome binding sites are the B0034 standard. The sequence is terminated by B0015, a double terminator. Mnt acts as the repressor.
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3129
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none
star: false
creator: Andrew Gordon
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K091163
component: BBa_K091107
sequence annotation: BBa_K091107
sequence annotation: BBa_K091163
sequence: https://synbiohub.org/public/igem/BBa_K091164_sequence/1

BBa_K091163
type: Component
persistent identity: component2266608
display id: component2266608
version: 1
top level: BBa_K091164
definition: BBa_K091163
access: public

BBa_K091107
type: Component
persistent identity: component2266587
display id: component2266587
version: 1
top level: BBa_K091164
definition: BBa_K091107
access: public

BBa_K091107
type: SequenceAnnotation
persistent identity: annotation2266587
display id: annotation2266587
version: 1
top level: BBa_K091164
location: https://synbiohub.org/public/igem/BBa_K091164/annotation2266587/range2266587/1
component: BBa_K091107

https://synbiohub.org/public/igem/BBa_K091164/annotation2266587/range2266587/1
type: Range
persistent identity: range2266587
display id: range2266587
version: 1
top level: BBa_K091164
direction: direction/reverse
start: 1
end: 57
orientation: inline

BBa_K091163
type: SequenceAnnotation
persistent identity: annotation2266608
display id: annotation2266608
version: 1
top level: BBa_K091164
location: https://synbiohub.org/public/igem/BBa_K091164/annotation2266608/range2266608/1
component: BBa_K091163

https://synbiohub.org/public/igem/BBa_K091164/annotation2266608/range2266608/1
type: Range
persistent identity: range2266608
display id: range2266608
version: 1
top level: BBa_K091164
direction: direction/reverse
start: 66
end: 1075
orientation: inline

BBa_K091107
type: ComponentDefinition
persistent identity: BBa K091107
display id: BBa_K091107
version: 1
was generated by: iGEM to SBOL conversion
description: pLux/cI Hybrid Promoter
created: 2008-06-18T11:00:00Z
modified: 2015-05-08T01:08:37Z
mutable provenance: this part comes from LuxR promoter and cI lambda promoter.
owned by: james
owned by: myers
mutable description: This hybrid promoter is designed to respond to activation by LuxR when it is bound to AHL and to repression by the phage lambda cI repressor. The repression needs to win over activation.
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3130
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: This part is based on the idea of replacing the region of the Lux promoter downstream of the -35 with the OR2 cI half-operator, added LuxR-10 region. Based on what we have found up to now, promoters which are constructed just by inserting OR1/2 downstream of Lux are all very leaky.
star: true
creator: Xiao Zhu
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: Lux Box
sequence annotation: Lux -35
sequence annotation: cI OR2
sequence annotation: Lux -10
sequence: https://synbiohub.org/public/igem/BBa_K091107_sequence/1

Lux Box
type: SequenceAnnotation
persistent identity: annotation1963750
display id: annotation1963750
version: 1
top level: BBa_K091107
location: https://synbiohub.org/public/igem/BBa_K091107/annotation1963750/range1963750/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_K091107/annotation1963750/range1963750/1
type: Range
persistent identity: range1963750
display id: range1963750
version: 1
top level: BBa_K091107
direction: direction/forward
start: 1
end: 20
orientation: inline

Lux -35
type: SequenceAnnotation
persistent identity: annotation1963751
display id: annotation1963751
version: 1
top level: BBa_K091107
location: https://synbiohub.org/public/igem/BBa_K091107/annotation1963751/range1963751/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K091107/annotation1963751/range1963751/1
type: Range
persistent identity: range1963751
display id: range1963751
version: 1
top level: BBa_K091107
direction: direction/forward
start: 20
end: 25
orientation: inline

cI OR2
type: SequenceAnnotation
persistent identity: annotation1963752
display id: annotation1963752
version: 1
top level: BBa_K091107
location: https://synbiohub.org/public/igem/BBa_K091107/annotation1963752/range1963752/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K091107/annotation1963752/range1963752/1
type: Range
persistent identity: range1963752
display id: range1963752
version: 1
top level: BBa_K091107
direction: direction/forward
start: 26
end: 43
orientation: inline

Lux -10
type: SequenceAnnotation
persistent identity: annotation1963753
display id: annotation1963753
version: 1
top level: BBa_K091107
location: https://synbiohub.org/public/igem/BBa_K091107/annotation1963753/range1963753/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K091107/annotation1963753/range1963753/1
type: Range
persistent identity: range1963753
display id: range1963753
version: 1
top level: BBa_K091107
direction: direction/forward
start: 44
end: 49
orientation: inline

BBa_K091163
type: ComponentDefinition
persistent identity: BBa K091163
display id: BBa_K091163
version: 1
was generated by: iGEM to SBOL conversion
description: RBS+LuxS+RBS+Mnt+TT
created: 2008-07-27T11:00:00Z
modified: 2015-05-08T01:08:38Z
mutable provenance: Registry
owned by: james
owned by: myers
mutable description: This is a coding sequence for LuxS, the AI-2 synthase, and the Mnt repressor. Both ribosome binding sites are the B0034 standard. At the terminal end is a double terminator, B0015.
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3129
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: Andrew Gordon
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K091153
component: BBa_K091137
sequence annotation: BBa_K091153
sequence annotation: BBa_K091137
sequence: https://synbiohub.org/public/igem/BBa_K091163_sequence/1

BBa_K091153
type: Component
persistent identity: component2261603
display id: component2261603
version: 1
top level: BBa_K091163
definition: BBa_K091153
access: public

BBa_K091137
type: Component
persistent identity: component2261619
display id: component2261619
version: 1
top level: BBa_K091163
definition: BBa_K091137
access: public

BBa_K091153
type: SequenceAnnotation
persistent identity: annotation2261603
display id: annotation2261603
version: 1
top level: BBa_K091163
location: https://synbiohub.org/public/igem/BBa_K091163/annotation2261603/range2261603/1
component: BBa_K091153

https://synbiohub.org/public/igem/BBa_K091163/annotation2261603/range2261603/1
type: Range
persistent identity: range2261603
display id: range2261603
version: 1
top level: BBa_K091163
direction: direction/reverse
start: 1
end: 534
orientation: inline

BBa_K091137
type: SequenceAnnotation
persistent identity: annotation2261619
display id: annotation2261619
version: 1
top level: BBa_K091163
location: https://synbiohub.org/public/igem/BBa_K091163/annotation2261619/range2261619/1
component: BBa_K091137

https://synbiohub.org/public/igem/BBa_K091163/annotation2261619/range2261619/1
type: Range
persistent identity: range2261619
display id: range2261619
version: 1
top level: BBa_K091163
direction: direction/reverse
start: 543
end: 1010
orientation: inline

BBa_K091137
type: ComponentDefinition
persistent identity: BBa K091137
display id: BBa_K091137
version: 1
was generated by: iGEM to SBOL conversion
description: RBS+Mnt+TT
created: 2008-07-13T11:00:00Z
modified: 2015-05-08T01:08:38Z
mutable provenance: These parts can be found in the registry.
owned by: james
owned by: myers
mutable description: This part was made by ligating I13025 to B0015.
discontinued: false
dominant: true
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: no special cosiderations
star: false
creator: Robert Cool
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0015
component: BBa_I13025
sequence annotation: BBa_I13025
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K091137_sequence/1

BBa_B0015
type: Component
persistent identity: component2249118
display id: component2249118
version: 1
top level: BBa_K091137
definition: BBa_B0015
access: public

BBa_I13025
type: Component
persistent identity: component2249111
display id: component2249111
version: 1
top level: BBa_K091137
definition: BBa_I13025
access: public

BBa_I13025
type: SequenceAnnotation
persistent identity: annotation2249111
display id: annotation2249111
version: 1
top level: BBa_K091137
location: https://synbiohub.org/public/igem/BBa_K091137/annotation2249111/range2249111/1
component: BBa_I13025

https://synbiohub.org/public/igem/BBa_K091137/annotation2249111/range2249111/1
type: Range
persistent identity: range2249111
display id: range2249111
version: 1
top level: BBa_K091137
direction: direction/reverse
start: 1
end: 331
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2249118
display id: annotation2249118
version: 1
top level: BBa_K091137
location: https://synbiohub.org/public/igem/BBa_K091137/annotation2249118/range2249118/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K091137/annotation2249118/range2249118/1
type: Range
persistent identity: range2249118
display id: range2249118
version: 1
top level: BBa_K091137
direction: direction/reverse
start: 340
end: 468
orientation: inline

BBa_K091153
type: ComponentDefinition
persistent identity: BBa K091153
display id: BBa_K091153
version: 1
was generated by: iGEM to SBOL conversion
description: Standard RBS with LuxS
created: 2008-07-20T11:00:00Z
modified: 2015-05-08T01:08:38Z
mutable provenance: B0034 came from the registry. K091109 is also in the registry, but it was built by the Davidson/Missouri Western team using oligos and PCR.
owned by: james
owned by: myers
mutable description: This is a composite part of B0034 and K091109. B0034 is the standard ribosome binding site. K091109 is the coding sequence for LuxS, which is the synthase for AI-2. Autoinducer 2 is used in quorum between bacteria of the same species, and it is also used to send signals to cells of other species.
discontinued: false
dominant: false
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 3129
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: None
star: false
creator: Andrew Gordon
type: DnaRegion
role: SO:0000804
role: partType/Signalling
component: BBa_K091109
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K091109
sequence: https://synbiohub.org/public/igem/BBa_K091153_sequence/1

BBa_K091109
type: Component
persistent identity: component1967772
display id: component1967772
version: 1
top level: BBa_K091153
definition: BBa_K091109
access: public

BBa_B0034
type: Component
persistent identity: component1967771
display id: component1967771
version: 1
top level: BBa_K091153
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1967771
display id: annotation1967771
version: 1
top level: BBa_K091153
location: https://synbiohub.org/public/igem/BBa_K091153/annotation1967771/range1967771/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K091153/annotation1967771/range1967771/1
type: Range
persistent identity: range1967771
display id: range1967771
version: 1
top level: BBa_K091153
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K091109
type: SequenceAnnotation
persistent identity: annotation1967772
display id: annotation1967772
version: 1
top level: BBa_K091153
location: https://synbiohub.org/public/igem/BBa_K091153/annotation1967772/range1967772/1
component: BBa_K091109

https://synbiohub.org/public/igem/BBa_K091153/annotation1967772/range1967772/1
type: Range
persistent identity: range1967772
display id: range1967772
version: 1
top level: BBa_K091153
direction: direction/reverse
start: 19
end: 534
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K091109
type: ComponentDefinition
persistent identity: BBa K091109
display id: BBa_K091109
version: 1
was generated by: iGEM to SBOL conversion
description: LuxS
created: 2008-06-18T11:00:00Z
modified: 2015-05-08T01:08:37Z
mutable provenance: The LuxS sequence was pulled from E. coli MG1655 by predesigned primers.
owned by: james
owned by: myers
mutable description: LuxS is a synthase for the AI-2 quorum sensing system. This protein is involved in the production of small molecules that are readily absorbed by multiple species of bacteria. LuxS can be used as part of a positive feedback loop in cell to cell signaling.
discontinued: false
dominant: false
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 3129
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Nucleotide position 54 changed from A to T in order to destroy a PstI site; this is a sense mutation Also changed stop codon from TAG to TAA (recommended in Registry Help)
star: false
creator: Andrew Gordon
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K091109_sequence/1

BBa_I13025
type: ComponentDefinition
persistent identity: BBa I13025
display id: BBa_I13025
version: 1
was generated by: iGEM to SBOL conversion
description: mnt QPI Intermediate
created: 2004-07-14T11:00:00Z
modified: 2015-08-31T04:07:32Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: jenmitch
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_C0072
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0072
sequence: https://synbiohub.org/public/igem/BBa_I13025_sequence/1

BBa_C0072
type: Component
persistent identity: component939424
display id: component939424
version: 1
top level: BBa_I13025
definition: mnt (s)
access: public

BBa_B0034
type: Component
persistent identity: component939411
display id: component939411
version: 1
top level: BBa_I13025
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation939411
display id: annotation939411
version: 1
top level: BBa_I13025
location: https://synbiohub.org/public/igem/BBa_I13025/annotation939411/range939411/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13025/annotation939411/range939411/1
type: Range
persistent identity: range939411
display id: range939411
version: 1
top level: BBa_I13025
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0072
type: SequenceAnnotation
persistent identity: annotation939424
display id: annotation939424
version: 1
top level: BBa_I13025
location: https://synbiohub.org/public/igem/BBa_I13025/annotation939424/range939424/1
component: BBa_C0072

https://synbiohub.org/public/igem/BBa_I13025/annotation939424/range939424/1
type: Range
persistent identity: range939424
display id: range939424
version: 1
top level: BBa_I13025
direction: direction/forward
start: 19
end: 306
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

mnt (s)
type: ComponentDefinition
persistent identity: BBa C0072
display id: BBa_C0072
version: 1
was generated by: iGEM to SBOL conversion
description: mnt repressor (strong) from Salmonella phage P22 (+LVA)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: enterobacteriophage p22
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: wild type dimeric repressor from enterobacteriophage p22 with dissociation constant of 2.5*10^-12. sequence was translated with the E.Coli K12 codon usage table, and nucleotides 95-97 was changed from AAT to AAC to avoid the cut site GAATTC (94-99). Identification of Functionally Important Residues in the DNA Binding Region of the MNT Repressor Kendall, KL; Sauer, RT The Journal of Biological Chemistry, 264(23):13706-13710, 1989
star: true
creator: crackdots
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mnt
sequence annotation: T
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0072_sequence/1

mnt
type: SequenceAnnotation
persistent identity: annotation308499
display id: annotation308499
version: 1
top level: mnt (s)
location: https://synbiohub.org/public/igem/BBa_C0072/annotation308499/range308499/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0072/annotation308499/range308499/1
type: Range
persistent identity: range308499
display id: range308499
version: 1
top level: mnt (s)
direction: direction/forward
start: 1
end: 249
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation308502
display id: annotation308502
version: 1
top level: mnt (s)
location: https://synbiohub.org/public/igem/BBa_C0072/annotation308502/range308502/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0072/annotation308502/range308502/1
type: Range
persistent identity: range308502
display id: range308502
version: 1
top level: mnt (s)
direction: direction/forward
start: 95
end: 97
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation308503
display id: annotation308503
version: 1
top level: mnt (s)
location: https://synbiohub.org/public/igem/BBa_C0072/annotation308503/range308503/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0072/annotation308503/range308503/1
type: Range
persistent identity: range308503
display id: range308503
version: 1
top level: mnt (s)
direction: direction/forward
start: 250
end: 282
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214010
display id: annotation2214010
version: 1
top level: mnt (s)
location: https://synbiohub.org/public/igem/BBa_C0072/annotation2214010/range2214010/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0072/annotation2214010/range2214010/1
type: Range
persistent identity: range2214010
display id: range2214010
version: 1
top level: mnt (s)
direction: direction/forward
start: 289
end: 313
orientation: inline

https://synbiohub.org/public/igem/BBa_K091164_sequence/1
type: Sequence
persistent identity: BBa K091164 sequence
display id: BBa_K091164_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgtaacaccgtgcgtgttgatatagtcgaataaatactagagaaagaggagaaatactagatgccgttgttagatagcttcacagtcgatcatacccggatggaagcgcctgctgttcgggtggcgaaaacaatgaacaccccgcatggcgacgcaatcaccgtgttcgatctgcgcttctgcgtgccgaacaaagaagtgatgccagaaagagggatccataccctggagcacctgtttgctggttttatgcgtaaccatcttaacggtaatggtgtagagattatcgatatctcgccaatgggctgccgcaccggtttttatatgagtctgattggtacgccagatgagcagcgtgttgctgatgcctggaaagcggcaatggaagacgtgctgaaagtgcaggatcagaatcagatcccggaactgaacgtctaccagtgtggcacttaccagatgcactcgttgcaggaagcgcaggatattgcgcgtagcattctggaacgtgacgtacgcatcaacagcaacgaagaactggcactgccgaaagagaagttgcaggaactgcacatctaatactagagaaagaggagaaatactagatggcccgggatgatcctcacttcaattttcgtatgccaatggaagtaagagagaaattgaaatttagagcagaggcaaacggacggagcatgaactctgagcttttgcaaatcgtacaagatgccctaagcaaaccgtcaccagtcactgggtaccgcaatgatgcggaacgactcgccgatgagcagagcgagttagtgaagaagatggtcttcgatacactgaaggatctttataaaaaaaccaccgctgcaaacgacgaaaactacgctttagtagcttaataaccctgatagtgctagtgtagatccctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K091107_sequence/1
type: Sequence
persistent identity: BBa K091107 sequence
display id: BBa_K091107_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgtaacaccgtgcgtgttgatatagtcgaataaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K091163_sequence/1
type: Sequence
persistent identity: BBa K091163 sequence
display id: BBa_K091163_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgccgttgttagatagcttcacagtcgatcatacccggatggaagcgcctgctgttcgggtggcgaaaacaatgaacaccccgcatggcgacgcaatcaccgtgttcgatctgcgcttctgcgtgccgaacaaagaagtgatgccagaaagagggatccataccctggagcacctgtttgctggttttatgcgtaaccatcttaacggtaatggtgtagagattatcgatatctcgccaatgggctgccgcaccggtttttatatgagtctgattggtacgccagatgagcagcgtgttgctgatgcctggaaagcggcaatggaagacgtgctgaaagtgcaggatcagaatcagatcccggaactgaacgtctaccagtgtggcacttaccagatgcactcgttgcaggaagcgcaggatattgcgcgtagcattctggaacgtgacgtacgcatcaacagcaacgaagaactggcactgccgaaagagaagttgcaggaactgcacatctaatactagagaaagaggagaaatactagatggcccgggatgatcctcacttcaattttcgtatgccaatggaagtaagagagaaattgaaatttagagcagaggcaaacggacggagcatgaactctgagcttttgcaaatcgtacaagatgccctaagcaaaccgtcaccagtcactgggtaccgcaatgatgcggaacgactcgccgatgagcagagcgagttagtgaagaagatggtcttcgatacactgaaggatctttataaaaaaaccaccgctgcaaacgacgaaaactacgctttagtagcttaataaccctgatagtgctagtgtagatccctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K091137_sequence/1
type: Sequence
persistent identity: BBa K091137 sequence
display id: BBa_K091137_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggcccgggatgatcctcacttcaattttcgtatgccaatggaagtaagagagaaattgaaatttagagcagaggcaaacggacggagcatgaactctgagcttttgcaaatcgtacaagatgccctaagcaaaccgtcaccagtcactgggtaccgcaatgatgcggaacgactcgccgatgagcagagcgagttagtgaagaagatggtcttcgatacactgaaggatctttataaaaaaaccaccgctgcaaacgacgaaaactacgctttagtagcttaataaccctgatagtgctagtgtagatccctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K091153_sequence/1
type: Sequence
persistent identity: BBa K091153 sequence
display id: BBa_K091153_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgccgttgttagatagcttcacagtcgatcatacccggatggaagcgcctgctgttcgggtggcgaaaacaatgaacaccccgcatggcgacgcaatcaccgtgttcgatctgcgcttctgcgtgccgaacaaagaagtgatgccagaaagagggatccataccctggagcacctgtttgctggttttatgcgtaaccatcttaacggtaatggtgtagagattatcgatatctcgccaatgggctgccgcaccggtttttatatgagtctgattggtacgccagatgagcagcgtgttgctgatgcctggaaagcggcaatggaagacgtgctgaaagtgcaggatcagaatcagatcccggaactgaacgtctaccagtgtggcacttaccagatgcactcgttgcaggaagcgcaggatattgcgcgtagcattctggaacgtgacgtacgcatcaacagcaacgaagaactggcactgccgaaagagaagttgcaggaactgcacatctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K091109_sequence/1
type: Sequence
persistent identity: BBa K091109 sequence
display id: BBa_K091109_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgccgttgttagatagcttcacagtcgatcatacccggatggaagcgcctgctgttcgggtggcgaaaacaatgaacaccccgcatggcgacgcaatcaccgtgttcgatctgcgcttctgcgtgccgaacaaagaagtgatgccagaaagagggatccataccctggagcacctgtttgctggttttatgcgtaaccatcttaacggtaatggtgtagagattatcgatatctcgccaatgggctgccgcaccggtttttatatgagtctgattggtacgccagatgagcagcgtgttgctgatgcctggaaagcggcaatggaagacgtgctgaaagtgcaggatcagaatcagatcccggaactgaacgtctaccagtgtggcacttaccagatgcactcgttgcaggaagcgcaggatattgcgcgtagcattctggaacgtgacgtacgcatcaacagcaacgaagaactggcactgccgaaagagaagttgcaggaactgcacatctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13025_sequence/1
type: Sequence
persistent identity: BBa I13025 sequence
display id: BBa_I13025_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggcccgggatgatcctcacttcaattttcgtatgccaatggaagtaagagagaaattgaaatttagagcagaggcaaacggacggagcatgaactctgagcttttgcaaatcgtacaagatgccctaagcaaaccgtcaccagtcactgggtaccgcaatgatgcggaacgactcgccgatgagcagagcgagttagtgaagaagatggtcttcgatacactgaaggatctttataaaaaaaccaccgctgcaaacgacgaaaactacgctttagtagcttaataaccctgatagtgctagtgtagatccc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0072_sequence/1
type: Sequence
persistent identity: BBa C0072 sequence
display id: BBa_C0072_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcccgggatgatcctcacttcaattttcgtatgccaatggaagtaagagagaaattgaaatttagagcagaggcaaacggacggagcatgaactctgagcttttgcaaatcgtacaagatgccctaagcaaaccgtcaccagtcactgggtaccgcaatgatgcggaacgactcgccgatgagcagagcgagttagtgaagaagatggtcttcgatacactgaaggatctttataaaaaaaccaccgctgcaaacgacgaaaactacgctttagtagcttaataaccctgatagtgctagtgtagatccc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z