This construct is a modified promoter attched to sequences for an activator and repressor. The activator is LuxS, the AI-2 synthase. Both ribosome binding sites are the B0034 standard. The sequence is terminated by B0015, a double terminator. Mnt acts as the repressor.
this part comes from LuxR promoter and cI lambda promoter.
This hybrid promoter is designed to respond to activation by LuxR when it is bound to AHL and to repression by the phage lambda cI repressor. The repression needs to win over activation.
This part is based on the idea of replacing the region of the Lux promoter downstream of the -35 with the OR2 cI half-operator, added LuxR-10 region. Based on what we have found up to now, promoters which are constructed just by inserting OR1/2 downstream of Lux are all very leaky.
This is a coding sequence for LuxS, the AI-2 synthase, and the Mnt repressor. Both ribosome binding sites are the B0034 standard. At the terminal end is a double terminator, B0015.
These parts can be found in the registry.
This part was made by ligating I13025 to B0015.
B0034 came from the registry. K091109 is also in the registry, but it was built by the Davidson/Missouri Western team using oligos and PCR.
This is a composite part of B0034 and K091109. B0034 is the standard ribosome binding site. K091109 is the coding sequence for LuxS, which is the synthase for AI-2. Autoinducer 2 is used in quorum between bacteria of the same species, and it is also used to send signals to cells of other species.
RBS (Elowitz 1999) -- defines RBS efficiency
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Double terminator consisting of BBa_B0010 and BBa_B0012
The LuxS sequence was pulled from E. coli MG1655 by predesigned primers.
LuxS is a synthase for the AI-2 quorum sensing system. This protein is involved in the production of small molecules that are readily absorbed by multiple species of bacteria. LuxS can be used as part of a positive feedback loop in cell to cell signaling.
Nucleotide position 54 changed from A to T in order to destroy a PstI site; this is a sense mutation Also changed stop codon from TAG to TAA (recommended in Registry Help)
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
mnt repressor (strong) from Salmonella phage P22 (+LVA)
wild type dimeric repressor from enterobacteriophage p22 with dissociation constant of 2.5*10^-12. sequence was translated with the E.Coli K12 codon usage table, and nucleotides 95-97 was changed from AAT to AAC to avoid the cut site GAATTC (94-99). Identification of Functionally Important Residues in the DNA Binding Region of the MNT Repressor Kendall, KL; Sauer, RT The Journal of Biological Chemistry, 264(23):13706-13710, 1989
Conversion of the iGEM parts registry to SBOL2.1