LOCUS       BBa_K091169             1048 bp    DNA     linear       24-Dec-2025
DEFINITION  plasR/cI+E0240(GFP)
ACCESSION   BBa_K091169
VERSION     BBa_K091169.1  
FEATURES             Location/Qualifiers
     promoter        140..145
                     /label=-10
     promoter        117..122
                     /label=-35
     misc_feature    147..164
                     /label=cI OR1
     misc_feature    46..63
                     /label=plasR OP1
     misc_feature    106..126
                     /label=plasR OP2
     promoter        1..164
                     /label=BBa_K091143
     RBS             173..185
                     /label=RBS-3\Weak
     RBS             179..182
                     /label=RBS
     misc_feature    173..185
                     /label=BBa_B0032
     RBS             173..185
                     /label=BBa_B0032
     CDS             192..911
                     /label=GFP protein
     CDS             192..911
                     /label=BBa_E0040
     stem_loop       931..974
                     /label=stem_loop
     misc_feature    920..999
                     /label=BBa_B0010
     terminator      920..999
                     /label=BBa_B0010
     stem_loop       1015..1034
                     /label=T7 TE
     misc_feature    1041..1041
                     /label=stop
     polyA_site      1035..1048
                     /label=polya
     misc_feature    1008..1048
                     /label=BBa_B0012
     terminator      1008..1048
                     /label=BBa_B0012
ORIGIN
        1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct
       61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg
      121 caggtttggc cgcgggttct ttttggtacc tctggcggtg ataatactag agtcacacag
      181 gaaagtacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg
      241 aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg
      301 caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat
      361 ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc
      421 atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa
      481 ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg
      541 atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc
      601 ttggacacaa attggaatac aactataact cacacaatgt atacatcatg gcagacaaac
      661 aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc
      721 aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag
      781 acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc
      841 acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat
      901 acaaataata atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
      961 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     1021 ccttcgggtg ggcctttctg cgtttata
//