LOCUS BBa_K091173 2965 bp DNA linear 22-Nov-2024 DEFINITION pLsr/cI+RBS+luxI+RBS+Mnt+TT+pTet/Mnt+RBS+luxI+RBS+cI+TT ACCESSION BBa_K091173 VERSION BBa_K091173.1 FEATURES Location/Qualifiers promoter 1..141 /label=BBa_K091106 misc_feature 154..157 /label=conserved RBS 150..161 /label=BBa_B0034 misc_feature 747..778 /label=LVA CDS 168..746 /label=luxI misc_feature 168..785 /label=BBa_C0061 misc_feature 786..810 /label=Help:Barcodes CDS 168..785 /label=BBa_C0061 misc_feature 150..810 /label=BBa_C0261 misc_feature 1..810 /label=BBa_K091140 misc_feature 823..826 /label=conserved RBS 819..830 /label=BBa_B0034 CDS 837..1085 /label=mnt misc_feature 931..933 /label=T misc_feature 1086..1118 /label=LVA misc_feature 1125..1149 /label=Help:Barcodes CDS 837..1124 /label=BBa_C0072 misc_feature 819..1149 /label=BBa_I13025 stem_loop 1169..1212 /label=stem_loop misc_feature 1158..1237 /label=BBa_B0010 terminator 1158..1237 /label=BBa_B0010 stem_loop 1253..1272 /label=T7 TE misc_feature 1279..1279 /label=stop polyA_site 1273..1286 /label=polya misc_feature 1246..1286 /label=BBa_B0012 terminator 1246..1286 /label=BBa_B0012 terminator 1158..1286 /label=BBa_B0015 misc_feature 819..1286 /label=BBa_K091141 misc_feature 1..1286 /label=BBa_K091142 promoter 1341..1346 /label=-10 misc_feature 1347..1365 /label=tetR1 misc_feature 1372..1392 /label=tetR2 misc_feature 1295..1346 /label=Mnt promoter 1295..1392 /label=BBa_K091105 misc_feature 1405..1408 /label=conserved RBS 1401..1412 /label=BBa_B0034 misc_feature 1998..2029 /label=LVA CDS 1419..1997 /label=luxI misc_feature 1419..2036 /label=BBa_C0061 misc_feature 2037..2061 /label=Help:Barcodes CDS 1419..2036 /label=BBa_C0061 misc_feature 1401..2061 /label=BBa_C0261 misc_feature 1295..2061 /label=BBa_K091132 misc_feature 2074..2077 /label=conserved RBS 2070..2081 /label=BBa_B0034 CDS 2091..2798 /label=cI lambda misc_feature 2799..2831 /label=LVA misc_feature 2838..2862 /label=Help:Barcodes CDS 2088..2837 /label=BBa_C0051 stem_loop 2878..2897 /label=T7 TE misc_feature 2904..2904 /label=stop polyA_site 2898..2911 /label=polya misc_feature 2871..2911 /label=BBa_B0012 terminator 2871..2911 /label=BBa_B0012 stem_loop 2932..2954 /label=stem_loop misc_feature 2920..2965 /label=BBa_B0011 terminator 2920..2965 /label=BBa_B0011 misc_feature 2070..2965 /label=BBa_P0455 misc_feature 1295..2965 /label=BBa_K091144 ORIGIN 1 aaccgtgaaa atcaaaatag cataaattgt gatctattcg tcggaaatat gtgcaatgtc 61 cacctaaggt tatgaacaaa ttaaaagcag aaatacattt aacaccgtgc gtgttgattt 121 atctaacacc gtgcgtgttg atactagaga aagaggagaa atactagatg actataatga 181 taaaaaaatc ggattttttg gcaattccat cggaggagta taaaggtatt ctaagtcttc 241 gttatcaagt gtttaagcaa agacttgagt gggacttagt tgtagaaaat aaccttgaat 301 cagatgagta tgataactca aatgcagaat atatttatgc ttgtgatgat actgaaaatg 361 taagtggatg ctggcgttta ttacctacaa caggtgatta tatgctgaaa agtgtttttc 421 ctgaattgct tggtcaacag agtgctccca aagatcctaa tatagtcgaa ttaagtcgtt 481 ttgctgtagg taaaaatagc tcaaagataa ataactctgc tagtgaaatt acaatgaaac 541 tatttgaagc tatatataaa cacgctgtta gtcaaggtat tacagaatat gtaacagtaa 601 catcaacagc aatagagcga tttttaaagc gtattaaagt tccttgtcat cgtattggag 661 acaaagaaat tcatgtatta ggtgatacta aatcggttgt attgtctatg cctattaatg 721 aacagtttaa aaaagcagtc ttaaatgctg caaacgacga aaactacgct ttagtagctt 781 aataactctg atagtgctag tgtagatctc tactagagaa agaggagaaa tactagatgg 841 cccgggatga tcctcacttc aattttcgta tgccaatgga agtaagagag aaattgaaat 901 ttagagcaga ggcaaacgga cggagcatga actctgagct tttgcaaatc gtacaagatg 961 ccctaagcaa accgtcacca gtcactgggt accgcaatga tgcggaacga ctcgccgatg 1021 agcagagcga gttagtgaag aagatggtct tcgatacact gaaggatctt tataaaaaaa 1081 ccaccgctgc aaacgacgaa aactacgctt tagtagctta ataaccctga tagtgctagt 1141 gtagatccct actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 1201 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc 1261 ttcgggtggg cctttctgcg tttatatact agagctcgag gtgagtgcac agtactaggt 1321 ccacggtgac ctagatctcc tatagttccc tatcagtgat agagaactgt aatccctatc 1381 agtgatagag attactagag aaagaggaga aatactagat gactataatg ataaaaaaat 1441 cggatttttt ggcaattcca tcggaggagt ataaaggtat tctaagtctt cgttatcaag 1501 tgtttaagca aagacttgag tgggacttag ttgtagaaaa taaccttgaa tcagatgagt 1561 atgataactc aaatgcagaa tatatttatg cttgtgatga tactgaaaat gtaagtggat 1621 gctggcgttt attacctaca acaggtgatt atatgctgaa aagtgttttt cctgaattgc 1681 ttggtcaaca gagtgctccc aaagatccta atatagtcga attaagtcgt tttgctgtag 1741 gtaaaaatag ctcaaagata aataactctg ctagtgaaat tacaatgaaa ctatttgaag 1801 ctatatataa acacgctgtt agtcaaggta ttacagaata tgtaacagta acatcaacag 1861 caatagagcg atttttaaag cgtattaaag ttccttgtca tcgtattgga gacaaagaaa 1921 ttcatgtatt aggtgatact aaatcggttg tattgtctat gcctattaat gaacagttta 1981 aaaaagcagt cttaaatgct gcaaacgacg aaaactacgc tttagtagct taataactct 2041 gatagtgcta gtgtagatct ctactagaga aagaggagaa atactagatg agcacaaaaa 2101 agaaaccatt aacacaagag cagcttgagg acgcacgtcg ccttaaagca atttatgaaa 2161 aaaagaaaaa tgaacttggc ttatcccagg aatctgtcgc agacaagatg gggatggggc 2221 agtcaggcgt tggtgcttta tttaatggca tcaatgcatt aaatgcttat aacgccgcat 2281 tgcttgcaaa aattctcaaa gttagcgttg aagaatttag cccttcaatc gccagagaaa 2341 tctacgagat gtatgaagcg gttagtatgc agccgtcact tagaagtgag tatgagtacc 2401 ctgttttttc tcatgttcag gcagggatgt tctcacctga gcttagaacc tttaccaaag 2461 gtgatgcgga gagatgggta agcacaacca aaaaagccag tgattctgca ttctggcttg 2521 aggttgaagg taattccatg accgcaccaa caggctccaa gccaagcttt cctgacggaa 2581 tgttaattct cgttgaccct gagcaggctg ttgagccagg tgatttctgc atagccagac 2641 ttgggggtga tgagtttacc ttcaagaaac tgatcaggga tagcggtcag gtgtttttac 2701 aaccactaaa cccacagtac ccaatgatcc catgcaatga gagttgttcc gttgtgggga 2761 aagttatcgc tagtcagtgg cctgaagaga cgtttggcgc tgcaaacgac gaaaactacg 2821 ctttagtagc ttaataacgc tgatagtgct agtgtagatc gctactagag tcacactggc 2881 tcaccttcgg gtgggccttt ctgcgtttat atactagaga gagaatataa aaagccagat 2941 tattaatccg gcttttttat tattt //