##gff-version 3
##sequence-region BBa_K091173 1 2965
BBa_K091173	.	engineered_region	1	1286	.	+	0	ID=BBa_K091142;Name=BBa_K091142
BBa_K091173	.	engineered_region	819	1286	.	+	0	ID=BBa_K091141;Name=BBa_K091141;Parent=BBa_K091142
BBa_K091173	.	engineered_region	819	1149	.	+	0	ID=BBa_I13025;Name=BBa_I13025;Parent=BBa_K091141
BBa_K091173	.	CDS	837	1124	.	+	0	ID=BBa_C0072;Name=BBa_C0072;Parent=BBa_I13025
BBa_K091173	.	point_mutation	931	933	.	+	0	ID=annotation308502;Name=T;Parent=BBa_C0072
BBa_K091173	.	tag	1086	1118	.	+	0	ID=annotation308503;Name=LVA;Parent=BBa_C0072
BBa_K091173	.	engineered_tag	1125	1149	.	+	0	ID=annotation2214010;Name=Help:Barcodes;Parent=BBa_C0072
BBa_K091173	.	CDS	837	1085	.	+	0	ID=annotation308499;Name=mnt;Parent=BBa_C0072
BBa_K091173	.	ribosome_entry_site	819	830	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13025
BBa_K091173	.	conserved_region	823	826	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K091173	.	terminator	1158	1286	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K091141
BBa_K091173	.	terminator	1158	1237	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K091173	.	engineered_region	1158	1237	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K091173	.	stem_loop	1169	1212	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K091173	.	terminator	1246	1286	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K091173	.	engineered_region	1246	1286	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K091173	.	stem_loop	1253	1272	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K091173	.	polyA_site	1273	1286	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K091173	.	stop_codon	1279	1279	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K091173	.	engineered_region	1	810	.	+	0	ID=BBa_K091140;Name=BBa_K091140;Parent=BBa_K091142
BBa_K091173	.	promoter	1	141	.	+	0	ID=BBa_K091106;Name=BBa_K091106;Parent=BBa_K091140
BBa_K091173	.	engineered_region	150	810	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_K091140
BBa_K091173	.	CDS	168	785	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K091173	.	engineered_tag	786	810	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K091173	.	engineered_region	168	785	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K091173	.	CDS	168	746	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K091173	.	tag	747	778	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K091173	.	ribosome_entry_site	150	161	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K091173	.	conserved_region	154	157	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K091173	.	engineered_region	1295	2965	.	+	0	ID=BBa_K091144;Name=BBa_K091144
BBa_K091173	.	engineered_region	1295	2061	.	+	0	ID=BBa_K091132;Name=BBa_K091132;Parent=BBa_K091144
BBa_K091173	.	promoter	1295	1392	.	+	0	ID=BBa_K091105;Name=BBa_K091105;Parent=BBa_K091132
BBa_K091173	.	operator	1295	1346	.	+	0	ID=annotation1963742;Name=Mnt;Parent=BBa_K091105
BBa_K091173	.	operator	1372	1392	.	+	0	ID=annotation1963741;Name=tetR2;Parent=BBa_K091105
BBa_K091173	.	operator	1347	1365	.	+	0	ID=annotation1963740;Name=tetR1;Parent=BBa_K091105
BBa_K091173	.	promoter	1341	1346	.	+	0	ID=annotation1963739;Name=-10;Parent=BBa_K091105
BBa_K091173	.	engineered_region	1401	2061	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_K091132
BBa_K091173	.	CDS	1419	2036	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K091173	.	engineered_tag	2037	2061	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K091173	.	engineered_region	1419	2036	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K091173	.	CDS	1419	1997	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K091173	.	tag	1998	2029	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K091173	.	ribosome_entry_site	1401	1412	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K091173	.	conserved_region	1405	1408	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K091173	.	engineered_region	2070	2965	.	+	0	ID=BBa_P0455;Name=BBa_P0455;Parent=BBa_K091144
BBa_K091173	.	CDS	2088	2837	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_P0455
BBa_K091173	.	engineered_tag	2838	2862	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K091173	.	tag	2799	2831	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K091173	.	CDS	2091	2798	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K091173	.	ribosome_entry_site	2070	2081	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0455
BBa_K091173	.	conserved_region	2074	2077	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K091173	.	terminator	2920	2965	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_P0455
BBa_K091173	.	engineered_region	2920	2965	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K091173	.	stem_loop	2932	2954	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K091173	.	terminator	2871	2911	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0455
BBa_K091173	.	engineered_region	2871	2911	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K091173	.	stem_loop	2878	2897	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K091173	.	polyA_site	2898	2911	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K091173	.	stop_codon	2904	2904	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
>BBa_K091173
aaccgtgaaaatcaaaatagcataaattgtgatctattcgtcggaaatatgtgcaatgtccacctaaggttatgaacaa
attaaaagcagaaatacatttaacaccgtgcgtgttgatttatctaacaccgtgcgtgttgatactagagaaagaggag
aaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtc
ttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataa
ctcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggt
gattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaa
gtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctat
atataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgt
attaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgc
ctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctg
atagtgctagtgtagatctctactagagaaagaggagaaatactagatggcccgggatgatcctcacttcaattttcgt
atgccaatggaagtaagagagaaattgaaatttagagcagaggcaaacggacggagcatgaactctgagcttttgcaaa
tcgtacaagatgccctaagcaaaccgtcaccagtcactgggtaccgcaatgatgcggaacgactcgccgatgagcagag
cgagttagtgaagaagatggtcttcgatacactgaaggatctttataaaaaaaccaccgctgcaaacgacgaaaactac
gctttagtagcttaataaccctgatagtgctagtgtagatccctactagagccaggcatcaaataaaacgaaaggctca
gtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcg
ggtgggcctttctgcgtttatatactagagctcgaggtgagtgcacagtactaggtccacggtgacctagatctcctat
agttccctatcagtgatagagaactgtaatccctatcagtgatagagattactagagaaagaggagaaatactagatga
ctataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagt
gtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaa
tatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctga
aaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgt
aggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgct
gttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttcctt
gtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaaca
gtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgt
agatctctactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacg
cacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggg
gatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaa
attctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgc
agccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaac
ctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaa
ggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcagg
ctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcgg
tcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagtt
atcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgat
agtgctagtgtagatcgctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagag
aatataaaaagccagattattaatccggcttttttattattt