##gff-version 3
##sequence-region BBa_K091174 1 3852
BBa_K091174	.	engineered_region	1	978	.	+	0	ID=BBa_K091145;Name=BBa_K091145
BBa_K091174	.	terminator	850	978	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K091145
BBa_K091174	.	terminator	850	929	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K091174	.	stem_loop	861	904	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K091174	.	engineered_region	850	929	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K091174	.	terminator	938	978	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K091174	.	polyA_site	965	978	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K091174	.	stem_loop	945	964	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K091174	.	engineered_region	938	978	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K091174	.	stop_codon	971	971	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K091174	.	engineered_region	1	841	.	+	0	ID=BBa_K091133;Name=BBa_K091133;Parent=BBa_K091145
BBa_K091174	.	promoter	1	130	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K091133
BBa_K091174	.	engineered_region	139	841	.	+	0	ID=BBa_S0101;Name=BBa_S0101;Parent=BBa_K091133
BBa_K091174	.	CDS	157	816	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_S0101
BBa_K091174	.	CDS	160	776	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K091174	.	tag	777	810	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K091174	.	engineered_tag	817	841	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K091174	.	ribosome_entry_site	139	150	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S0101
BBa_K091174	.	conserved_region	143	146	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K091174	.	engineered_region	987	3852	.	+	0	ID=BBa_K091172;Name=BBa_K091172
BBa_K091174	.	CDS	1143	2735	.	+	0	ID=BBa_K091002;Name=BBa_K091002;Parent=BBa_K091172
BBa_K091174	.	ribosome_entry_site	1125	1136	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K091172
BBa_K091174	.	conserved_region	1129	1132	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K091174	.	CDS	2762	3715	.	+	0	ID=BBa_K091001;Name=BBa_K091001;Parent=BBa_K091172
BBa_K091174	.	terminator	3724	3803	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K091172
BBa_K091174	.	stem_loop	3735	3778	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K091174	.	engineered_region	3724	3803	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K091174	.	terminator	3812	3852	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K091172
BBa_K091174	.	polyA_site	3839	3852	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K091174	.	stem_loop	3819	3838	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K091174	.	engineered_region	3812	3852	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K091174	.	stop_codon	3845	3845	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K091174	.	promoter	987	1116	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K091172
BBa_K091174	.	ribosome_entry_site	2744	2755	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K091172
BBa_K091174	.	conserved_region	2748	2751	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K091174
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagat
gtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacc
cgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacg
ccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacg
taataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctaca
gaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatg
cactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaaggga
aacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagcc
ttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacg
aaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacga
aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct
caccttcgggtgggcctttctgcgtttatatactagagacattgattatttgcacggcgtcacactttgctatgccata
gcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgttttt
ttgggctagctactagagaaagaggagaaatactagatggctcgactctttaccctttcagaatcaaagtactacctga
tggcgctggatgcaggcaccggaagtattcgggctgtgatattcgacctggaaggcaatcaaatagcagtgggacaggc
ggagtggcggcatctggcagtaccggacgttcctggttctatggaatttgatctcaacaaaaactggcaactggcgtgt
gagtgtatgcgccaggcgctgcacaacgccggcatagccccggagtatatcgctgccgtttcggcatgttcgatgcgtg
aaggcattgttttatataataatgaaggagccccgatctgggcctgcgccaatgtggatgccagagcggcacgcgaagt
tagcgaacttaaagaactgcacaacaatacctttgaaaacgaagtttatcgcgcgaccggacaaacactggctttaagt
gccatccccagattactttggctggcgcaccatcgttccgatatttaccgtcaggcatcaaccatcaccatgatcagcg
actggctggcctatatgctcagcggcgaactggcggtggatccctctaacgctggcaccacgggacttcttgatctaac
cacccgtgactggaaacctgcattgctggatatggctggcctacgtgccgatattctttctcctgtcaaagaaaccggc
acattgctgggcgtggtaagttcacaagcggcggaactctgcggtctgaaggcgggcactccggtggtcgttggaggag
gcgacgtgcagcttggttgccttgggttaggcgttgtgcgtccggcacaaaccgcggttcttggcggcacattctggca
gcaagttgtaaatttagccgcgccggtgacagacccagaaatgaacgtgcgcgttaatcctcatgttattcctggcatg
gtacaagctgaatctataagcttttttaccggactcaccatgcgctggttccgcgatgctttctgtgccgaagaaaaac
tgattgccgaacgtttaggcatcgacacctatacgctgctggaagagatggccagtcgggtgccgcctgggtcgtgggg
cgtaatgccgatcttctccgacagaatgcgctttaaaacctggtatcacgctgcgccttcctttattaacttgtccatt
gacccggataaatgtaacaaagcgacattgttccgtgcgctggaagaaaatgcggcgattgtatcagcgtgtaacttgc
agcaaattgctgatttctcgaatattcatccttcatcgttagtctttgcaggcggaggttcaaaagggaaattatggag
tcaaattctcgctgatgtctcgggattacccgtcaatattccggtggtcaaagaagccactgcattaggatgtgccatt
gcagctggcgtcggtgccggaattttttcatcaatggcagaaaccggagaacgcctggttcgctgggaacggacgcaca
caccagacccggaaaagcatgaactttatcaggattcacgcgataagtggcaggcagtttatcaggatcagctggggct
ggttgatcatggactgacgacgtcgttatggaaagcgcctgggttatagtactagagaaagaggagaaatactagatga
caatcaacgattcggcaatttcagaacagggaatgtgtgaagaagaacaggtcgcgcggatcgcgtggttttactatca
cgacgggctgacccagagcgagatcagcgatcgtctcggcctgacacgtttgaaagtgtcgcgattgctggagaaaggg
catcagtccggcattattcgcgtacagattaattctcgctttgaaggctgtctggaatatgaaactcaattacgtcgtc
agttttcgctgcaacatgtccgggtgatccctgggcttgcggatgctgatgtcggtgggcgactggggataggcgcggc
gcatatgttgatgagtttacttcaaccacaacagatgctggcgattggttttggcgaggcaaccatgaatacgctgcaa
cgcttaagtggttttatttcgtcacagcaaattcgcctggtcacgctctccggtggcgtcggttcttatatgacgggaa
tcgggcagcttaacgcggcgtgcagtgtgaatattattccggctccgttgcgggcatcctccgctgacattgcccgtac
gctaaaaaatgaaaattgcgtcaaagatgttctgttagccgcgcaagcagcggatgtggcgattgtcggcattggtgct
gtgagtcaacaggacgatgcgacaatcattcgctccggttatatcagccagggcgaacagttaatgattggccgaaaag
gggcggttggcgacattttaggctacttttttgatgcaaaaggtgacgttgtcacgaatatcaaaatacataacgaact
gattggcttacctttaagcgcgctgaagaccatacccgtccgggttggcgtggcagggggagaaaataaagccgaagca
attgccgctgcaatgaaaggcggttatatcaacgcactggttaccgatcaggacacagcagcggcgattttacgtagtt
aatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcgg
tgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata