BBa_K091174
type: ComponentDefinition
persistent identity: BBa K091174
display id: BBa_K091174
version: 1
was generated by: iGEM to SBOL conversion
description: pBAD-RBS-TetR-TT-pBAD-RBS-LsrK-RBS-LsrR-TT
created: 2008-08-03T11:00:00Z
modified: 2015-05-08T01:08:38Z
mutable provenance: Construction intermediates.
owned by: james
owned by: myers
mutable description: This construct is designed for constitutive expression of TetR, LsrK, and LsrR. TetR produces a repressor that can be derepressed by aTc. LsrK produces a kinase that phosphorylates AI-2. LsrR produces a repressor that can be derepressed by phosphorylated AI-2.
discontinued: false
dominant: false
experience: experience/None
group u list: _120_191_71_
m user id: 0
owner id: 606
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None.
star: false
creator: Todd Eckdahl
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K091145
component: BBa_K091172
sequence annotation: BBa_K091145
sequence annotation: BBa_K091172
sequence: https://synbiohub.org/public/igem/BBa_K091174_sequence/1

BBa_K091145
type: Component
persistent identity: component2296951
display id: component2296951
version: 1
top level: BBa_K091174
definition: BBa_K091145
access: public

BBa_K091172
type: Component
persistent identity: component2296965
display id: component2296965
version: 1
top level: BBa_K091174
definition: BBa_K091172
access: public

BBa_K091145
type: SequenceAnnotation
persistent identity: annotation2296951
display id: annotation2296951
version: 1
top level: BBa_K091174
location: https://synbiohub.org/public/igem/BBa_K091174/annotation2296951/range2296951/1
component: BBa_K091145

https://synbiohub.org/public/igem/BBa_K091174/annotation2296951/range2296951/1
type: Range
persistent identity: range2296951
display id: range2296951
version: 1
top level: BBa_K091174
direction: direction/reverse
start: 1
end: 978
orientation: inline

BBa_K091172
type: SequenceAnnotation
persistent identity: annotation2296965
display id: annotation2296965
version: 1
top level: BBa_K091174
location: https://synbiohub.org/public/igem/BBa_K091174/annotation2296965/range2296965/1
component: BBa_K091172

https://synbiohub.org/public/igem/BBa_K091174/annotation2296965/range2296965/1
type: Range
persistent identity: range2296965
display id: range2296965
version: 1
top level: BBa_K091174
direction: direction/reverse
start: 987
end: 3852
orientation: inline

BBa_K091145
type: ComponentDefinition
persistent identity: BBa K091145
display id: BBa_K091145
version: 1
was generated by: iGEM to SBOL conversion
description: pBAD+RBS+TetR+TT
created: 2008-07-15T11:00:00Z
modified: 2015-05-08T01:08:38Z
mutable provenance: These parts are in the registry.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This parts was made by ligating K091133 to B0015.
discontinued: false
dominant: true
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: no special considerations
star: false
creator: Robert Cool
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_K091133
sequence annotation: BBa_K091133
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K091145_sequence/1

BBa_B0015
type: Component
persistent identity: component2254196
display id: component2254196
version: 1
top level: BBa_K091145
definition: BBa_B0015
access: public

BBa_K091133
type: Component
persistent identity: component2254189
display id: component2254189
version: 1
top level: BBa_K091145
definition: BBa_K091133
access: public

BBa_K091133
type: SequenceAnnotation
persistent identity: annotation2254189
display id: annotation2254189
version: 1
top level: BBa_K091145
location: https://synbiohub.org/public/igem/BBa_K091145/annotation2254189/range2254189/1
component: BBa_K091133

https://synbiohub.org/public/igem/BBa_K091145/annotation2254189/range2254189/1
type: Range
persistent identity: range2254189
display id: range2254189
version: 1
top level: BBa_K091145
direction: direction/reverse
start: 1
end: 841
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2254196
display id: annotation2254196
version: 1
top level: BBa_K091145
location: https://synbiohub.org/public/igem/BBa_K091145/annotation2254196/range2254196/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K091145/annotation2254196/range2254196/1
type: Range
persistent identity: range2254196
display id: range2254196
version: 1
top level: BBa_K091145
direction: direction/reverse
start: 850
end: 978
orientation: inline

BBa_K091172
type: ComponentDefinition
persistent identity: BBa K091172
display id: BBa_K091172
version: 1
was generated by: iGEM to SBOL conversion
description: lsr system supporting genes
created: 2008-07-30T11:00:00Z
modified: 2015-05-08T01:08:38Z
mutable provenance: E. coli MG1655
owned by: james
owned by: myers
mutable description: pBAD+RBS+lsrK+RBS+lsrR+TT
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3130
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: .
star: false
creator: Xiao Zhu
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_I13453
component: BBa_K091001
component: BBa_B0034
component: BBa_K091002
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_I13453
sequence annotation: BBa_B0034
sequence annotation: BBa_K091002
sequence annotation: BBa_K091001
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K091172_sequence/1

BBa_I13453
type: Component
persistent identity: component1969568
display id: component1969568
version: 1
top level: BBa_K091172
definition: BBa_I13453
access: public

BBa_K091001
type: Component
persistent identity: component1969574
display id: component1969574
version: 1
top level: BBa_K091172
definition: lsrR
access: public

BBa_B0034
type: Component
persistent identity: component1969573
display id: component1969573
version: 1
top level: BBa_K091172
definition: BBa_B0034
access: public

BBa_K091002
type: Component
persistent identity: component1969571
display id: component1969571
version: 1
top level: BBa_K091172
definition: lsrK
access: public

BBa_B0034
type: Component
persistent identity: component1969570
display id: component1969570
version: 1
top level: BBa_K091172
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component1969577
display id: component1969577
version: 1
top level: BBa_K091172
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1969575
display id: component1969575
version: 1
top level: BBa_K091172
definition: BBa_B0010
access: public

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation1969568
display id: annotation1969568
version: 1
top level: BBa_K091172
location: https://synbiohub.org/public/igem/BBa_K091172/annotation1969568/range1969568/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K091172/annotation1969568/range1969568/1
type: Range
persistent identity: range1969568
display id: range1969568
version: 1
top level: BBa_K091172
direction: direction/reverse
start: 1
end: 130
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1969570
display id: annotation1969570
version: 1
top level: BBa_K091172
location: https://synbiohub.org/public/igem/BBa_K091172/annotation1969570/range1969570/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K091172/annotation1969570/range1969570/1
type: Range
persistent identity: range1969570
display id: range1969570
version: 1
top level: BBa_K091172
direction: direction/reverse
start: 139
end: 150
orientation: inline

BBa_K091002
type: SequenceAnnotation
persistent identity: annotation1969571
display id: annotation1969571
version: 1
top level: BBa_K091172
location: https://synbiohub.org/public/igem/BBa_K091172/annotation1969571/range1969571/1
component: BBa_K091002

https://synbiohub.org/public/igem/BBa_K091172/annotation1969571/range1969571/1
type: Range
persistent identity: range1969571
display id: range1969571
version: 1
top level: BBa_K091172
direction: direction/reverse
start: 157
end: 1749
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1969573
display id: annotation1969573
version: 1
top level: BBa_K091172
location: https://synbiohub.org/public/igem/BBa_K091172/annotation1969573/range1969573/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K091172/annotation1969573/range1969573/1
type: Range
persistent identity: range1969573
display id: range1969573
version: 1
top level: BBa_K091172
direction: direction/reverse
start: 1758
end: 1769
orientation: inline

BBa_K091001
type: SequenceAnnotation
persistent identity: annotation1969574
display id: annotation1969574
version: 1
top level: BBa_K091172
location: https://synbiohub.org/public/igem/BBa_K091172/annotation1969574/range1969574/1
component: BBa_K091001

https://synbiohub.org/public/igem/BBa_K091172/annotation1969574/range1969574/1
type: Range
persistent identity: range1969574
display id: range1969574
version: 1
top level: BBa_K091172
direction: direction/reverse
start: 1776
end: 2729
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1969575
display id: annotation1969575
version: 1
top level: BBa_K091172
location: https://synbiohub.org/public/igem/BBa_K091172/annotation1969575/range1969575/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K091172/annotation1969575/range1969575/1
type: Range
persistent identity: range1969575
display id: range1969575
version: 1
top level: BBa_K091172
direction: direction/forward
start: 2738
end: 2817
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1969577
display id: annotation1969577
version: 1
top level: BBa_K091172
location: https://synbiohub.org/public/igem/BBa_K091172/annotation1969577/range1969577/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K091172/annotation1969577/range1969577/1
type: Range
persistent identity: range1969577
display id: range1969577
version: 1
top level: BBa_K091172
direction: direction/forward
start: 2826
end: 2866
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_I13453
type: ComponentDefinition
persistent identity: BBa I13453
display id: BBa_I13453
version: 1
was generated by: iGEM to SBOL conversion
description: Pbad promoter
created: 2005-05-24T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: PBad promoter from I0500 without AraC.
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I13453_sequence/1

lsrR
type: ComponentDefinition
persistent identity: BBa K091001
display id: BBa_K091001
version: 1
was generated by: iGEM to SBOL conversion
description: LsrR gene
created: 2008-06-18T11:00:00Z
modified: 2015-05-08T01:08:37Z
mutable provenance: The DNA for this part is found in the E. coli strain MG1655.
owned by: james
owned by: myers
mutable description: This sequence codes for the LsrR gene which is found in the E. coli strain MG1655. When this protein fors a complex with phosphorylated AI-2 it promotes the LsrA promoter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: no special considerations
star: false
creator: Robert Cool
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K091001_sequence/1

lsrK
type: ComponentDefinition
persistent identity: BBa K091002
display id: BBa_K091002
version: 1
was generated by: iGEM to SBOL conversion
description: LsrK gene
created: 2008-06-18T11:00:00Z
modified: 2015-05-08T01:08:37Z
mutable provenance: This sequence is found in the E. coli strain MG1655.
owned by: james
owned by: myers
mutable description: This part codes for the protein LsrK which phosphorylates AI-2 which acts to promote the lsrA promoter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: no special considerations
star: false
creator: Robert Cool
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K091002_sequence/1

BBa_K091133
type: ComponentDefinition
persistent identity: BBa K091133
display id: BBa_K091133
version: 1
was generated by: iGEM to SBOL conversion
description: pBAD+RBS+TetR
created: 2008-07-10T11:00:00Z
modified: 2015-05-08T01:08:38Z
mutable provenance: These parts are in the registry.
owned by: james
owned by: myers
mutable description: This part was made by ligating I13453 (pBAD) to S0101 (RBS+TetR).
discontinued: false
dominant: false
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: no special considerations
star: false
creator: Robert Cool
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_S0101
component: BBa_I13453
sequence annotation: BBa_I13453
sequence annotation: BBa_S0101
sequence: https://synbiohub.org/public/igem/BBa_K091133_sequence/1

BBa_S0101
type: Component
persistent identity: component2245892
display id: component2245892
version: 1
top level: BBa_K091133
definition: BBa_S0101
access: public

BBa_I13453
type: Component
persistent identity: component2245885
display id: component2245885
version: 1
top level: BBa_K091133
definition: BBa_I13453
access: public

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2245885
display id: annotation2245885
version: 1
top level: BBa_K091133
location: https://synbiohub.org/public/igem/BBa_K091133/annotation2245885/range2245885/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K091133/annotation2245885/range2245885/1
type: Range
persistent identity: range2245885
display id: range2245885
version: 1
top level: BBa_K091133
direction: direction/reverse
start: 1
end: 130
orientation: inline

BBa_S0101
type: SequenceAnnotation
persistent identity: annotation2245892
display id: annotation2245892
version: 1
top level: BBa_K091133
location: https://synbiohub.org/public/igem/BBa_K091133/annotation2245892/range2245892/1
component: BBa_S0101

https://synbiohub.org/public/igem/BBa_K091133/annotation2245892/range2245892/1
type: Range
persistent identity: range2245892
display id: range2245892
version: 1
top level: BBa_K091133
direction: direction/reverse
start: 139
end: 841
orientation: inline

BBa_S0101
type: ComponentDefinition
persistent identity: BBa S0101
display id: BBa_S0101
version: 1
was generated by: iGEM to SBOL conversion
description: B0034.C0040
created: 2003-12-04T12:00:00Z
modified: 2015-05-08T01:14:16Z
owned by: james
owned by: myers
part status: Released HQ 2013
discontinued: false
dominant: true
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_C0040
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0040
sequence: https://synbiohub.org/public/igem/BBa_S0101_sequence/1

BBa_C0040
type: Component
persistent identity: component939723
display id: component939723
version: 1
top level: BBa_S0101
definition: tetR
access: public

BBa_B0034
type: Component
persistent identity: component939713
display id: component939713
version: 1
top level: BBa_S0101
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation939713
display id: annotation939713
version: 1
top level: BBa_S0101
location: https://synbiohub.org/public/igem/BBa_S0101/annotation939713/range939713/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S0101/annotation939713/range939713/1
type: Range
persistent identity: range939713
display id: range939713
version: 1
top level: BBa_S0101
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation939723
display id: annotation939723
version: 1
top level: BBa_S0101
location: https://synbiohub.org/public/igem/BBa_S0101/annotation939723/range939723/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_S0101/annotation939723/range939723/1
type: Range
persistent identity: range939723
display id: range939723
version: 1
top level: BBa_S0101
direction: direction/forward
start: 19
end: 678
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa C0040
display id: BBa_C0040
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline repressor from transposon Tn10 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: tetR
sequence annotation: SsrA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0040_sequence/1

tetR
type: SequenceAnnotation
persistent identity: annotation23329
display id: annotation23329
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
type: Range
persistent identity: range23329
display id: range23329
version: 1
top level: tetR
direction: direction/forward
start: 4
end: 620
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation23330
display id: annotation23330
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
type: Range
persistent identity: range23330
display id: range23330
version: 1
top level: tetR
direction: direction/forward
start: 621
end: 654
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213989
display id: annotation2213989
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
type: Range
persistent identity: range2213989
display id: range2213989
version: 1
top level: tetR
direction: direction/forward
start: 661
end: 685
orientation: inline

https://synbiohub.org/public/igem/BBa_K091174_sequence/1
type: Sequence
persistent identity: BBa K091174 sequence
display id: BBa_K091174_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatggctcgactctttaccctttcagaatcaaagtactacctgatggcgctggatgcaggcaccggaagtattcgggctgtgatattcgacctggaaggcaatcaaatagcagtgggacaggcggagtggcggcatctggcagtaccggacgttcctggttctatggaatttgatctcaacaaaaactggcaactggcgtgtgagtgtatgcgccaggcgctgcacaacgccggcatagccccggagtatatcgctgccgtttcggcatgttcgatgcgtgaaggcattgttttatataataatgaaggagccccgatctgggcctgcgccaatgtggatgccagagcggcacgcgaagttagcgaacttaaagaactgcacaacaatacctttgaaaacgaagtttatcgcgcgaccggacaaacactggctttaagtgccatccccagattactttggctggcgcaccatcgttccgatatttaccgtcaggcatcaaccatcaccatgatcagcgactggctggcctatatgctcagcggcgaactggcggtggatccctctaacgctggcaccacgggacttcttgatctaaccacccgtgactggaaacctgcattgctggatatggctggcctacgtgccgatattctttctcctgtcaaagaaaccggcacattgctgggcgtggtaagttcacaagcggcggaactctgcggtctgaaggcgggcactccggtggtcgttggaggaggcgacgtgcagcttggttgccttgggttaggcgttgtgcgtccggcacaaaccgcggttcttggcggcacattctggcagcaagttgtaaatttagccgcgccg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K091145_sequence/1
type: Sequence
persistent identity: BBa K091145 sequence
display id: BBa_K091145_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K091172_sequence/1
type: Sequence
persistent identity: BBa K091172 sequence
display id: BBa_K091172_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatggctcgactctttaccctttcagaatcaaagtactacctgatggcgctggatgcaggcaccggaagtattcgggctgtgatattcgacctggaaggcaatcaaatagcagtgggacaggcggagtggcggcatctggcagtaccggacgttcctggttctatggaatttgatctcaacaaaaactggcaactggcgtgtgagtgtatgcgccaggcgctgcacaacgccggcatagccccggagtatatcgctgccgtttcggcatgttcgatgcgtgaaggcattgttttatataataatgaaggagccccgatctgggcctgcgccaatgtggatgccagagcggcacgcgaagttagcgaacttaaagaactgcacaacaatacctttgaaaacgaagtttatcgcgcgaccggacaaacactggctttaagtgccatccccagattactttggctggcgcaccatcgttccgatatttaccgtcaggcatcaaccatcaccatgatcagcgactggctggcctatatgctcagcggcgaactggcggtggatccctctaacgctggcaccacgggacttcttgatctaaccacccgtgactggaaacctgcattgctggatatggctggcctacgtgccgatattctttctcctgtcaaagaaaccggcacattgctgggcgtggtaagttcacaagcggcggaactctgcggtctgaaggcgggcactccggtggtcgttggaggaggcgacgtgcagcttggttgccttgggttaggcgttgtgcgtccggcacaaaccgcggttcttggcggcacattctggcagcaagttgtaaatttagccgcgccggtgacagacccagaaatgaacgtgcgcgttaatcctcatgttattcctggcatggtacaagctgaatctataagcttttttaccggactcaccatgcgctggttccgcgatgctttctgtgccgaagaaaaactgattgccgaacgtttaggcatcgacacctatacgctgctggaagagatggccagtcgggtgccgcctgggtcgtggggcgtaatgccgatcttctccgacagaatgcgctttaaaacctggtatcacgctgcgccttcctttattaacttgtccattgacccggataaatgtaacaaagcgacattgttccgtgcgctggaagaaaatgcggcgattgtatcagcgtgtaacttgcagcaaattgctgatttctcgaatattcatccttcatcgttagtctttgcaggcggaggttcaaaagggaaattatggagtcaaattctcgctgatgtctcgggattacccgtcaatattccggtggtcaaagaagccactgcattaggatgtgccattgcagctggcgtcggtgccggaattttttcatcaatggcagaaaccggagaacgcctggttcgctgggaacggacgcacacaccagacccggaaaagcatgaactttatcaggattcacgcgataagtggcaggcagtttatcaggatcagctggggctggttgatcatggactgacgacgtcgttatggaaagcgcctgggttatagtactagagaaagaggagaaatactagatgacaatcaacgattcggcaatttcagaacagggaatgtgtgaagaagaacaggtcgcgcggatcgcgtggttttactatcacgacgggctgacccagagcgagatcagcgatcgtctcggcctgacacgtttgaaagtgtcgcgattgctggagaaagggcatcagtccggcattattcgcgtacagattaattctcgctttgaaggctgtctggaatatgaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13453_sequence/1
type: Sequence
persistent identity: BBa I13453 sequence
display id: BBa_I13453_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K091001_sequence/1
type: Sequence
persistent identity: BBa K091001 sequence
display id: BBa_K091001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgacaatcaacgattcggcaatttcagaacagggaatgtgtgaagaagaacaggtcgcgcggatcgcgtggttttactatcacgacgggctgacccagagcgagatcagcgatcgtctcggcctgacacgtttgaaagtgtcgcgattgctggagaaagggcatcagtccggcattattcgcgtacagattaattctcgctttgaaggctgtctggaatatgaaactcaattacgtcgtcagttttcgctgcaacatgtccgggtgatccctgggcttgcggatgctgatgtcggtgggcgactggggataggcgcggcgcatatgttgatgagtttacttcaaccacaacagatgctggcgattggttttggcgaggcaaccatgaatacgctgcaacgcttaagtggttttatttcgtcacagcaaattcgcctggtcacgctctccggtggcgtcggttcttatatgacgggaatcgggcagcttaacgcggcgtgcagtgtgaatattattccggctccgttgcgggcatcctccgctgacattgcccgtacgctaaaaaatgaaaattgcgtcaaagatgttctgttagccgcgcaagcagcggatgtggcgattgtcggcattggtgctgtgagtcaacaggacgatgcgacaatcattcgctccggttatatcagccagggcgaacagttaatgattggccgaaaaggggcggttggcgacattttaggctacttttttgatgcaaaaggtgacgttgtcacgaatatcaaaatacataacgaactgattggcttacctttaagcgcgctgaagaccatacccgtccgggttggcgtggcagggggagaaaataaagccgaagcaattgccgctgcaatgaaaggcggttatatcaacgcactggttaccgatcaggacacagcagcggcgattttacgtagttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K091002_sequence/1
type: Sequence
persistent identity: BBa K091002 sequence
display id: BBa_K091002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctcgactctttaccctttcagaatcaaagtactacctgatggcgctggatgcaggcaccggaagtattcgggctgtgatattcgacctggaaggcaatcaaatagcagtgggacaggcggagtggcggcatctggcagtaccggacgttcctggttctatggaatttgatctcaacaaaaactggcaactggcgtgtgagtgtatgcgccaggcgctgcacaacgccggcatagccccggagtatatcgctgccgtttcggcatgttcgatgcgtgaaggcattgttttatataataatgaaggagccccgatctgggcctgcgccaatgtggatgccagagcggcacgcgaagttagcgaacttaaagaactgcacaacaatacctttgaaaacgaagtttatcgcgcgaccggacaaacactggctttaagtgccatccccagattactttggctggcgcaccatcgttccgatatttaccgtcaggcatcaaccatcaccatgatcagcgactggctggcctatatgctcagcggcgaactggcggtggatccctctaacgctggcaccacgggacttcttgatctaaccacccgtgactggaaacctgcattgctggatatggctggcctacgtgccgatattctttctcctgtcaaagaaaccggcacattgctgggcgtggtaagttcacaagcggcggaactctgcggtctgaaggcgggcactccggtggtcgttggaggaggcgacgtgcagcttggttgccttgggttaggcgttgtgcgtccggcacaaaccgcggttcttggcggcacattctggcagcaagttgtaaatttagccgcgccggtgacagacccagaaatgaacgtgcgcgttaatcctcatgttattcctggcatggtacaagctgaatctataagcttttttaccggactcaccatgcgctggttccgcgatgctttctgtgccgaagaaaaactgattgccgaacgtttaggcatcgacacctatacgctgctggaagagatggccagtcgggtgccgcctgggtcgtggggcgtaatgccgatcttctccgacagaatgcgctttaaaacctggtatcacgctgcgccttcctttattaacttgtccattgacccggataaatgtaacaaagcgacattgttccgtgcgctggaagaaaatgcggcgattgtatcagcgtgtaacttgcagcaaattgctgatttctcgaatattcatccttcatcgttagtctttgcaggcggaggttcaaaagggaaattatggagtcaaattctcgctgatgtctcgggattacccgtcaatattccggtggtcaaagaagccactgcattaggatgtgccattgcagctggcgtcggtgccggaattttttcatcaatggcagaaaccggagaacgcctggttcgctgggaacggacgcacacaccagacccggaaaagcatgaactttatcaggattcacgcgataagtggcaggcagtttatcaggatcagctggggctggttgatcatggactgacgacgtcgttatggaaagcgcctgggttatag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K091133_sequence/1
type: Sequence
persistent identity: BBa K091133 sequence
display id: BBa_K091133_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S0101_sequence/1
type: Sequence
persistent identity: BBa S0101 sequence
display id: BBa_S0101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0040_sequence/1
type: Sequence
persistent identity: BBa C0040 sequence
display id: BBa_C0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z