LOCUS       BBa_K091175             1968 bp    DNA     linear       16-Feb-2025
DEFINITION  pBAD-RBS-tetR-TT-pMnt/tetR-RBS-GFP-TT
ACCESSION   BBa_K091175
VERSION     BBa_K091175.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=BBa_I13453
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     CDS             160..776
                     /label=tetR
     misc_feature    777..810
                     /label=SsrA
     misc_feature    817..841
                     /label=Help:Barcodes
     CDS             157..816
                     /label=BBa_C0040
     misc_feature    139..841
                     /label=BBa_S0101
     misc_feature    1..841
                     /label=BBa_K091133
     stem_loop       861..904
                     /label=stem_loop
     misc_feature    850..929
                     /label=BBa_B0010
     terminator      850..929
                     /label=BBa_B0010
     stem_loop       945..964
                     /label=T7 TE
     misc_feature    971..971
                     /label=stop
     polyA_site      965..978
                     /label=polya
     misc_feature    938..978
                     /label=BBa_B0012
     terminator      938..978
                     /label=BBa_B0012
     terminator      850..978
                     /label=BBa_B0015
     misc_feature    1..978
                     /label=BBa_K091145
     promoter        1033..1038
                     /label=-10
     misc_feature    1039..1057
                     /label=tetR1
     misc_feature    1064..1084
                     /label=tetR2
     misc_feature    987..1038
                     /label=Mnt
     promoter        987..1084
                     /label=BBa_K091105
     RBS             1093..1105
                     /label=RBS-3\Weak
     RBS             1099..1102
                     /label=RBS
     misc_feature    1093..1105
                     /label=BBa_B0032
     RBS             1093..1105
                     /label=BBa_B0032
     CDS             1112..1831
                     /label=GFP protein
     CDS             1112..1831
                     /label=BBa_E0040
     stem_loop       1851..1894
                     /label=stem_loop
     misc_feature    1840..1919
                     /label=BBa_B0010
     terminator      1840..1919
                     /label=BBa_B0010
     stem_loop       1935..1954
                     /label=T7 TE
     misc_feature    1961..1961
                     /label=stop
     polyA_site      1955..1968
                     /label=polya
     misc_feature    1928..1968
                     /label=BBa_B0012
     terminator      1928..1968
                     /label=BBa_B0012
     misc_feature    987..1968
                     /label=BBa_K091160
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatgt ccagattaga taaaagtaaa
      181 gtgattaaca gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt
      241 aaactcgccc agaagctagg tgtagagcag cctacattgt attggcatgt aaaaaataag
      301 cgggctttgc tcgacgcctt agccattgag atgttagata ggcaccatac tcacttttgc
      361 cctttagaag gggaaagctg gcaagatttt ttacgtaata acgctaaaag ttttagatgt
      421 gctttactaa gtcatcgcga tggagcaaaa gtacatttag gtacacggcc tacagaaaaa
      481 cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt ttcactagag
      541 aatgcattat atgcactcag cgctgtgggg cattttactt taggttgcgt attggaagat
      601 caagagcatc aagtcgctaa agaagaaagg gaaacaccta ctactgatag tatgccgcca
      661 ttattacgac aagctatcga attatttgat caccaaggtg cagagccagc cttcttattc
      721 ggccttgaat tgatcatatg cggattagaa aaacaactta aatgtgaaag tgggtccgct
      781 gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta gtgtagatca
      841 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
      901 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
      961 ggcctttctg cgtttatata ctagagctcg aggtgagtgc acagtactag gtccacggtg
     1021 acctagatct cctatagttc cctatcagtg atagagaact gtaatcccta tcagtgatag
     1081 agattactag agtcacacag gaaagtacta gatgcgtaaa ggagaagaac ttttcactgg
     1141 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag
     1201 tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac
     1261 tggaaaacta cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg
     1321 ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga
     1381 aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc
     1441 tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt
     1501 taaagaagat ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt
     1561 atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa
     1621 cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga
     1681 tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga
     1741 tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac
     1801 acatggcatg gatgaactat acaaataata atactagagc caggcatcaa ataaaacgaa
     1861 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     1921 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//