##gff-version 3
##sequence-region BBa_K091175 1 1968
BBa_K091175	.	engineered_region	1	978	.	+	0	ID=BBa_K091145;Name=BBa_K091145
BBa_K091175	.	engineered_region	1	841	.	+	0	ID=BBa_K091133;Name=BBa_K091133;Parent=BBa_K091145
BBa_K091175	.	promoter	1	130	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K091133
BBa_K091175	.	engineered_region	139	841	.	+	0	ID=BBa_S0101;Name=BBa_S0101;Parent=BBa_K091133
BBa_K091175	.	CDS	157	816	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_S0101
BBa_K091175	.	engineered_tag	817	841	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K091175	.	CDS	160	776	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K091175	.	tag	777	810	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K091175	.	ribosome_entry_site	139	150	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S0101
BBa_K091175	.	conserved_region	143	146	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K091175	.	terminator	850	978	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K091145
BBa_K091175	.	terminator	938	978	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K091175	.	polyA_site	965	978	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K091175	.	stem_loop	945	964	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K091175	.	stop_codon	971	971	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K091175	.	engineered_region	938	978	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K091175	.	terminator	850	929	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K091175	.	stem_loop	861	904	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K091175	.	engineered_region	850	929	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K091175	.	engineered_region	987	1968	.	+	0	ID=BBa_K091160;Name=BBa_K091160
BBa_K091175	.	terminator	1840	1919	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K091160
BBa_K091175	.	stem_loop	1851	1894	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K091175	.	engineered_region	1840	1919	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K091175	.	promoter	987	1084	.	+	0	ID=BBa_K091105;Name=BBa_K091105;Parent=BBa_K091160
BBa_K091175	.	operator	987	1038	.	+	0	ID=annotation1963742;Name=Mnt;Parent=BBa_K091105
BBa_K091175	.	operator	1064	1084	.	+	0	ID=annotation1963741;Name=tetR2;Parent=BBa_K091105
BBa_K091175	.	promoter	1033	1038	.	+	0	ID=annotation1963739;Name=-10;Parent=BBa_K091105
BBa_K091175	.	operator	1039	1057	.	+	0	ID=annotation1963740;Name=tetR1;Parent=BBa_K091105
BBa_K091175	.	CDS	1112	1831	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_K091160
BBa_K091175	.	CDS	1112	1831	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K091175	.	ribosome_entry_site	1093	1105	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K091160
BBa_K091175	.	engineered_region	1093	1105	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K091175	.	ribosome_entry_site	1099	1102	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K091175	.	ribosome_entry_site	1093	1105	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K091175	.	terminator	1928	1968	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K091160
BBa_K091175	.	polyA_site	1955	1968	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K091175	.	stem_loop	1935	1954	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K091175	.	stop_codon	1961	1961	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K091175	.	engineered_region	1928	1968	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K091175
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagat
gtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacc
cgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacg
ccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacg
taataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctaca
gaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatg
cactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaaggga
aacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagcc
ttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacg
aaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacga
aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct
caccttcgggtgggcctttctgcgtttatatactagagctcgaggtgagtgcacagtactaggtccacggtgacctaga
tctcctatagttccctatcagtgatagagaactgtaatccctatcagtgatagagattactagagtcacacaggaaagt
actagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggca
caaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactgga
aaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcata
tgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgg
gaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgatttt
aaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaac
aaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatca
acaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaa
gatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaac
tatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatct
gttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata