BBa_K091175
type: ComponentDefinition
persistent identity: BBa K091175
display id: BBa_K091175
version: 1
was generated by: iGEM to SBOL conversion
description: pBAD-RBS-tetR-TT-pMnt/tetR-RBS-GFP-TT
created: 2008-08-03T11:00:00Z
modified: 2015-05-08T01:08:38Z
mutable provenance: Both of these parts contain parts from the registry. K091160 contains DNA that was built from oligos.
owned by: myers
owned by: james
mutable description: This part was made by ligating K091145 to the 5' end of K091160.
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: no special considerations
star: false
creator: Robert Cool
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K091145
component: BBa_K091160
sequence annotation: BBa_K091145
sequence annotation: BBa_K091160
sequence: https://synbiohub.org/public/igem/BBa_K091175_sequence/1

BBa_K091145
type: Component
persistent identity: component2281458
display id: component2281458
version: 1
top level: BBa_K091175
definition: BBa_K091145
access: public

BBa_K091160
type: Component
persistent identity: component2281475
display id: component2281475
version: 1
top level: BBa_K091175
definition: BBa_K091160
access: public

BBa_K091145
type: SequenceAnnotation
persistent identity: annotation2281458
display id: annotation2281458
version: 1
top level: BBa_K091175
location: https://synbiohub.org/public/igem/BBa_K091175/annotation2281458/range2281458/1
component: BBa_K091145

https://synbiohub.org/public/igem/BBa_K091175/annotation2281458/range2281458/1
type: Range
persistent identity: range2281458
display id: range2281458
version: 1
top level: BBa_K091175
direction: direction/reverse
start: 1
end: 978
orientation: inline

BBa_K091160
type: SequenceAnnotation
persistent identity: annotation2281475
display id: annotation2281475
version: 1
top level: BBa_K091175
location: https://synbiohub.org/public/igem/BBa_K091175/annotation2281475/range2281475/1
component: BBa_K091160

https://synbiohub.org/public/igem/BBa_K091175/annotation2281475/range2281475/1
type: Range
persistent identity: range2281475
display id: range2281475
version: 1
top level: BBa_K091175
direction: direction/reverse
start: 987
end: 1968
orientation: inline

BBa_K091145
type: ComponentDefinition
persistent identity: BBa K091145
display id: BBa_K091145
version: 1
was generated by: iGEM to SBOL conversion
description: pBAD+RBS+TetR+TT
created: 2008-07-15T11:00:00Z
modified: 2015-05-08T01:08:38Z
mutable provenance: These parts are in the registry.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This parts was made by ligating K091133 to B0015.
discontinued: false
dominant: true
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: no special considerations
star: false
creator: Robert Cool
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_K091133
sequence annotation: BBa_K091133
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K091145_sequence/1

BBa_B0015
type: Component
persistent identity: component2254196
display id: component2254196
version: 1
top level: BBa_K091145
definition: BBa_B0015
access: public

BBa_K091133
type: Component
persistent identity: component2254189
display id: component2254189
version: 1
top level: BBa_K091145
definition: BBa_K091133
access: public

BBa_K091133
type: SequenceAnnotation
persistent identity: annotation2254189
display id: annotation2254189
version: 1
top level: BBa_K091145
location: https://synbiohub.org/public/igem/BBa_K091145/annotation2254189/range2254189/1
component: BBa_K091133

https://synbiohub.org/public/igem/BBa_K091145/annotation2254189/range2254189/1
type: Range
persistent identity: range2254189
display id: range2254189
version: 1
top level: BBa_K091145
direction: direction/reverse
start: 1
end: 841
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2254196
display id: annotation2254196
version: 1
top level: BBa_K091145
location: https://synbiohub.org/public/igem/BBa_K091145/annotation2254196/range2254196/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K091145/annotation2254196/range2254196/1
type: Range
persistent identity: range2254196
display id: range2254196
version: 1
top level: BBa_K091145
direction: direction/reverse
start: 850
end: 978
orientation: inline

BBa_K091160
type: ComponentDefinition
persistent identity: BBa K091160
display id: BBa_K091160
version: 1
was generated by: iGEM to SBOL conversion
description: pMnt/Tet-RBS-GFP-TT
created: 2008-07-27T11:00:00Z
modified: 2015-05-08T01:08:38Z
mutable provenance: E0240 can be found in the registry, K091105 was built from oligos.
owned by: james
owned by: myers
mutable description: This part was made by ligating K091105 with E0240.
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: no special considerations
star: false
creator: Robert Cool
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E0040
component: BBa_B0032
component: BBa_K091105
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_K091105
sequence annotation: BBa_B0032
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K091160_sequence/1

BBa_E0040
type: Component
persistent identity: component1968926
display id: component1968926
version: 1
top level: BBa_K091160
definition: GFP
access: public

BBa_B0032
type: Component
persistent identity: component1968923
display id: component1968923
version: 1
top level: BBa_K091160
definition: BBa_B0032
access: public

BBa_K091105
type: Component
persistent identity: component1968921
display id: component1968921
version: 1
top level: BBa_K091160
definition: BBa_K091105
access: public

BBa_B0012
type: Component
persistent identity: component1968929
display id: component1968929
version: 1
top level: BBa_K091160
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1968927
display id: component1968927
version: 1
top level: BBa_K091160
definition: BBa_B0010
access: public

BBa_K091105
type: SequenceAnnotation
persistent identity: annotation1968921
display id: annotation1968921
version: 1
top level: BBa_K091160
location: https://synbiohub.org/public/igem/BBa_K091160/annotation1968921/range1968921/1
component: BBa_K091105

https://synbiohub.org/public/igem/BBa_K091160/annotation1968921/range1968921/1
type: Range
persistent identity: range1968921
display id: range1968921
version: 1
top level: BBa_K091160
direction: direction/reverse
start: 1
end: 98
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1968923
display id: annotation1968923
version: 1
top level: BBa_K091160
location: https://synbiohub.org/public/igem/BBa_K091160/annotation1968923/range1968923/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K091160/annotation1968923/range1968923/1
type: Range
persistent identity: range1968923
display id: range1968923
version: 1
top level: BBa_K091160
direction: direction/forward
start: 107
end: 119
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1968926
display id: annotation1968926
version: 1
top level: BBa_K091160
location: https://synbiohub.org/public/igem/BBa_K091160/annotation1968926/range1968926/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K091160/annotation1968926/range1968926/1
type: Range
persistent identity: range1968926
display id: range1968926
version: 1
top level: BBa_K091160
direction: direction/reverse
start: 126
end: 845
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1968927
display id: annotation1968927
version: 1
top level: BBa_K091160
location: https://synbiohub.org/public/igem/BBa_K091160/annotation1968927/range1968927/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K091160/annotation1968927/range1968927/1
type: Range
persistent identity: range1968927
display id: range1968927
version: 1
top level: BBa_K091160
direction: direction/forward
start: 854
end: 933
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1968929
display id: annotation1968929
version: 1
top level: BBa_K091160
location: https://synbiohub.org/public/igem/BBa_K091160/annotation1968929/range1968929/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K091160/annotation1968929/range1968929/1
type: Range
persistent identity: range1968929
display id: range1968929
version: 1
top level: BBa_K091160
direction: direction/forward
start: 942
end: 982
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K091105
type: ComponentDefinition
persistent identity: BBa K091105
display id: BBa_K091105
version: 1
was generated by: iGEM to SBOL conversion
description: pTet/Mnt Hybrid Promoter
created: 2008-06-18T11:00:00Z
modified: 2015-05-08T01:08:37Z
mutable provenance: This part comes from the Mnt promoter and the TetR promoter
owned by: james
owned by: myers
mutable description: This promoter is a modified version of the Mnt promoter that is also responsive to TetR. The promoter should be repressed by Mnt repressor. It should also be repressed by TetR, and in the absence of Mnt repressor, should be induced by aTc.
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Mnt repressor binds as a tetramer to two half-operator sites. Introduction of TetR binding sites between the Mnt promoter -10 sequence and the start site for transcription should allow for repression by TetR. Accordingly, the hybrid promoter was designed by 1) Remove all bases of mnt promoter 3??? to -10: gagtcgtattaattt will be replaced by tccctatcagtgata, and 2) truncate the second half of ptet -10 and mutate -35 so that it function to bind tetR (replace ttgaca with actgta), but is not a promoter, and 3) place the tetR1 and tetR2 binding sites 3??? to truncated mnt -10 sequence.
star: true
creator: Robert Cool
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Mnt
sequence annotation: -10
sequence annotation: tetR1
sequence annotation: tetR2
sequence: https://synbiohub.org/public/igem/BBa_K091105_sequence/1

Mnt
type: SequenceAnnotation
persistent identity: annotation1963742
display id: annotation1963742
version: 1
top level: BBa_K091105
location: https://synbiohub.org/public/igem/BBa_K091105/annotation1963742/range1963742/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K091105/annotation1963742/range1963742/1
type: Range
persistent identity: range1963742
display id: range1963742
version: 1
top level: BBa_K091105
direction: direction/forward
start: 1
end: 52
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1963739
display id: annotation1963739
version: 1
top level: BBa_K091105
location: https://synbiohub.org/public/igem/BBa_K091105/annotation1963739/range1963739/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K091105/annotation1963739/range1963739/1
type: Range
persistent identity: range1963739
display id: range1963739
version: 1
top level: BBa_K091105
direction: direction/forward
start: 47
end: 52
orientation: inline

tetR1
type: SequenceAnnotation
persistent identity: annotation1963740
display id: annotation1963740
version: 1
top level: BBa_K091105
location: https://synbiohub.org/public/igem/BBa_K091105/annotation1963740/range1963740/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K091105/annotation1963740/range1963740/1
type: Range
persistent identity: range1963740
display id: range1963740
version: 1
top level: BBa_K091105
direction: direction/forward
start: 53
end: 71
orientation: inline

tetR2
type: SequenceAnnotation
persistent identity: annotation1963741
display id: annotation1963741
version: 1
top level: BBa_K091105
location: https://synbiohub.org/public/igem/BBa_K091105/annotation1963741/range1963741/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K091105/annotation1963741/range1963741/1
type: Range
persistent identity: range1963741
display id: range1963741
version: 1
top level: BBa_K091105
direction: direction/forward
start: 78
end: 98
orientation: inline

BBa_K091133
type: ComponentDefinition
persistent identity: BBa K091133
display id: BBa_K091133
version: 1
was generated by: iGEM to SBOL conversion
description: pBAD+RBS+TetR
created: 2008-07-10T11:00:00Z
modified: 2015-05-08T01:08:38Z
mutable provenance: These parts are in the registry.
owned by: james
owned by: myers
mutable description: This part was made by ligating I13453 (pBAD) to S0101 (RBS+TetR).
discontinued: false
dominant: false
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: no special considerations
star: false
creator: Robert Cool
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_S0101
component: BBa_I13453
sequence annotation: BBa_I13453
sequence annotation: BBa_S0101
sequence: https://synbiohub.org/public/igem/BBa_K091133_sequence/1

BBa_S0101
type: Component
persistent identity: component2245892
display id: component2245892
version: 1
top level: BBa_K091133
definition: BBa_S0101
access: public

BBa_I13453
type: Component
persistent identity: component2245885
display id: component2245885
version: 1
top level: BBa_K091133
definition: BBa_I13453
access: public

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2245885
display id: annotation2245885
version: 1
top level: BBa_K091133
location: https://synbiohub.org/public/igem/BBa_K091133/annotation2245885/range2245885/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K091133/annotation2245885/range2245885/1
type: Range
persistent identity: range2245885
display id: range2245885
version: 1
top level: BBa_K091133
direction: direction/reverse
start: 1
end: 130
orientation: inline

BBa_S0101
type: SequenceAnnotation
persistent identity: annotation2245892
display id: annotation2245892
version: 1
top level: BBa_K091133
location: https://synbiohub.org/public/igem/BBa_K091133/annotation2245892/range2245892/1
component: BBa_S0101

https://synbiohub.org/public/igem/BBa_K091133/annotation2245892/range2245892/1
type: Range
persistent identity: range2245892
display id: range2245892
version: 1
top level: BBa_K091133
direction: direction/reverse
start: 139
end: 841
orientation: inline

BBa_I13453
type: ComponentDefinition
persistent identity: BBa I13453
display id: BBa_I13453
version: 1
was generated by: iGEM to SBOL conversion
description: Pbad promoter
created: 2005-05-24T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: PBad promoter from I0500 without AraC.
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I13453_sequence/1

BBa_S0101
type: ComponentDefinition
persistent identity: BBa S0101
display id: BBa_S0101
version: 1
was generated by: iGEM to SBOL conversion
description: B0034.C0040
created: 2003-12-04T12:00:00Z
modified: 2015-05-08T01:14:16Z
owned by: james
owned by: myers
part status: Released HQ 2013
discontinued: false
dominant: true
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_C0040
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0040
sequence: https://synbiohub.org/public/igem/BBa_S0101_sequence/1

BBa_C0040
type: Component
persistent identity: component939723
display id: component939723
version: 1
top level: BBa_S0101
definition: tetR
access: public

BBa_B0034
type: Component
persistent identity: component939713
display id: component939713
version: 1
top level: BBa_S0101
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation939713
display id: annotation939713
version: 1
top level: BBa_S0101
location: https://synbiohub.org/public/igem/BBa_S0101/annotation939713/range939713/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S0101/annotation939713/range939713/1
type: Range
persistent identity: range939713
display id: range939713
version: 1
top level: BBa_S0101
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation939723
display id: annotation939723
version: 1
top level: BBa_S0101
location: https://synbiohub.org/public/igem/BBa_S0101/annotation939723/range939723/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_S0101/annotation939723/range939723/1
type: Range
persistent identity: range939723
display id: range939723
version: 1
top level: BBa_S0101
direction: direction/forward
start: 19
end: 678
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa C0040
display id: BBa_C0040
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline repressor from transposon Tn10 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: tetR
sequence annotation: SsrA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0040_sequence/1

tetR
type: SequenceAnnotation
persistent identity: annotation23329
display id: annotation23329
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
type: Range
persistent identity: range23329
display id: range23329
version: 1
top level: tetR
direction: direction/forward
start: 4
end: 620
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation23330
display id: annotation23330
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
type: Range
persistent identity: range23330
display id: range23330
version: 1
top level: tetR
direction: direction/forward
start: 621
end: 654
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213989
display id: annotation2213989
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
type: Range
persistent identity: range2213989
display id: range2213989
version: 1
top level: tetR
direction: direction/forward
start: 661
end: 685
orientation: inline

https://synbiohub.org/public/igem/BBa_K091175_sequence/1
type: Sequence
persistent identity: BBa K091175 sequence
display id: BBa_K091175_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagctcgaggtgagtgcacagtactaggtccacggtgacctagatctcctatagttccctatcagtgatagagaactgtaatccctatcagtgatagagattactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K091145_sequence/1
type: Sequence
persistent identity: BBa K091145 sequence
display id: BBa_K091145_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K091160_sequence/1
type: Sequence
persistent identity: BBa K091160 sequence
display id: BBa_K091160_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcgaggtgagtgcacagtactaggtccacggtgacctagatctcctatagttccctatcagtgatagagaactgtaatccctatcagtgatagagattactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K091105_sequence/1
type: Sequence
persistent identity: BBa K091105 sequence
display id: BBa_K091105_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcgaggtgagtgcacagtactaggtccacggtgacctagatctcctatagttccctatcagtgatagagaactgtaatccctatcagtgatagagat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K091133_sequence/1
type: Sequence
persistent identity: BBa K091133 sequence
display id: BBa_K091133_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13453_sequence/1
type: Sequence
persistent identity: BBa I13453 sequence
display id: BBa_I13453_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S0101_sequence/1
type: Sequence
persistent identity: BBa S0101 sequence
display id: BBa_S0101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0040_sequence/1
type: Sequence
persistent identity: BBa C0040 sequence
display id: BBa_C0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z