LOCUS       BBa_K091176             1596 bp    DNA     linear       25-Nov-2024
DEFINITION  pBAD-RBS-Mnt-TT-pMnt/tetR-RBS-GFP-TT
ACCESSION   BBa_K091176
VERSION     BBa_K091176.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=BBa_I13453
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     CDS             157..405
                     /label=mnt
     misc_feature    251..253
                     /label=T
     misc_feature    406..438
                     /label=LVA
     misc_feature    445..469
                     /label=Help:Barcodes
     CDS             157..444
                     /label=BBa_C0072
     misc_feature    139..469
                     /label=BBa_I13025
     stem_loop       489..532
                     /label=stem_loop
     misc_feature    478..557
                     /label=BBa_B0010
     terminator      478..557
                     /label=BBa_B0010
     stem_loop       573..592
                     /label=T7 TE
     misc_feature    599..599
                     /label=stop
     polyA_site      593..606
                     /label=polya
     misc_feature    566..606
                     /label=BBa_B0012
     terminator      566..606
                     /label=BBa_B0012
     terminator      478..606
                     /label=BBa_B0015
     misc_feature    139..606
                     /label=BBa_K091137
     misc_feature    1..606
                     /label=BBa_K091147
     promoter        661..666
                     /label=-10
     misc_feature    667..685
                     /label=tetR1
     misc_feature    692..712
                     /label=tetR2
     misc_feature    615..666
                     /label=Mnt
     promoter        615..712
                     /label=BBa_K091105
     RBS             721..733
                     /label=RBS-3\Weak
     RBS             727..730
                     /label=RBS
     misc_feature    721..733
                     /label=BBa_B0032
     RBS             721..733
                     /label=BBa_B0032
     CDS             740..1459
                     /label=GFP protein
     CDS             740..1459
                     /label=BBa_E0040
     stem_loop       1479..1522
                     /label=stem_loop
     misc_feature    1468..1547
                     /label=BBa_B0010
     terminator      1468..1547
                     /label=BBa_B0010
     stem_loop       1563..1582
                     /label=T7 TE
     misc_feature    1589..1589
                     /label=stop
     polyA_site      1583..1596
                     /label=polya
     misc_feature    1556..1596
                     /label=BBa_B0012
     terminator      1556..1596
                     /label=BBa_B0012
     misc_feature    615..1596
                     /label=BBa_K091160
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatgg cccgggatga tcctcacttc
      181 aattttcgta tgccaatgga agtaagagag aaattgaaat ttagagcaga ggcaaacgga
      241 cggagcatga actctgagct tttgcaaatc gtacaagatg ccctaagcaa accgtcacca
      301 gtcactgggt accgcaatga tgcggaacga ctcgccgatg agcagagcga gttagtgaag
      361 aagatggtct tcgatacact gaaggatctt tataaaaaaa ccaccgctgc aaacgacgaa
      421 aactacgctt tagtagctta ataaccctga tagtgctagt gtagatccct actagagcca
      481 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
      541 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
      601 tttatatact agagctcgag gtgagtgcac agtactaggt ccacggtgac ctagatctcc
      661 tatagttccc tatcagtgat agagaactgt aatccctatc agtgatagag attactagag
      721 tcacacagga aagtactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat
      781 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
      841 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
      901 tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata
      961 cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca
     1021 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt
     1081 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
     1141 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc
     1201 agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg
     1261 aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct
     1321 tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa
     1381 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga
     1441 tgaactatac aaataataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     1501 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     1561 ctggctcacc ttcgggtggg cctttctgcg tttata
//