##gff-version 3
##sequence-region BBa_K091176 1 1596
BBa_K091176	.	engineered_region	1	606	.	+	0	ID=BBa_K091147;Name=BBa_K091147
BBa_K091176	.	engineered_region	139	606	.	+	0	ID=BBa_K091137;Name=BBa_K091137;Parent=BBa_K091147
BBa_K091176	.	engineered_region	139	469	.	+	0	ID=BBa_I13025;Name=BBa_I13025;Parent=BBa_K091137
BBa_K091176	.	ribosome_entry_site	139	150	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13025
BBa_K091176	.	conserved_region	143	146	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K091176	.	CDS	157	444	.	+	0	ID=BBa_C0072;Name=BBa_C0072;Parent=BBa_I13025
BBa_K091176	.	CDS	157	405	.	+	0	ID=annotation308499;Name=mnt;Parent=BBa_C0072
BBa_K091176	.	tag	406	438	.	+	0	ID=annotation308503;Name=LVA;Parent=BBa_C0072
BBa_K091176	.	point_mutation	251	253	.	+	0	ID=annotation308502;Name=T;Parent=BBa_C0072
BBa_K091176	.	engineered_tag	445	469	.	+	0	ID=annotation2214010;Name=Help:Barcodes;Parent=BBa_C0072
BBa_K091176	.	terminator	478	606	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K091137
BBa_K091176	.	terminator	566	606	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K091176	.	polyA_site	593	606	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K091176	.	stem_loop	573	592	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K091176	.	stop_codon	599	599	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K091176	.	engineered_region	566	606	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K091176	.	terminator	478	557	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K091176	.	stem_loop	489	532	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K091176	.	engineered_region	478	557	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K091176	.	promoter	1	130	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K091147
BBa_K091176	.	engineered_region	615	1596	.	+	0	ID=BBa_K091160;Name=BBa_K091160
BBa_K091176	.	terminator	1468	1547	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K091160
BBa_K091176	.	stem_loop	1479	1522	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K091176	.	engineered_region	1468	1547	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K091176	.	promoter	615	712	.	+	0	ID=BBa_K091105;Name=BBa_K091105;Parent=BBa_K091160
BBa_K091176	.	operator	615	666	.	+	0	ID=annotation1963742;Name=Mnt;Parent=BBa_K091105
BBa_K091176	.	operator	692	712	.	+	0	ID=annotation1963741;Name=tetR2;Parent=BBa_K091105
BBa_K091176	.	promoter	661	666	.	+	0	ID=annotation1963739;Name=-10;Parent=BBa_K091105
BBa_K091176	.	operator	667	685	.	+	0	ID=annotation1963740;Name=tetR1;Parent=BBa_K091105
BBa_K091176	.	CDS	740	1459	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_K091160
BBa_K091176	.	CDS	740	1459	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K091176	.	ribosome_entry_site	721	733	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K091160
BBa_K091176	.	engineered_region	721	733	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K091176	.	ribosome_entry_site	727	730	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K091176	.	ribosome_entry_site	721	733	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K091176	.	terminator	1556	1596	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K091160
BBa_K091176	.	polyA_site	1583	1596	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K091176	.	stem_loop	1563	1582	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K091176	.	stop_codon	1589	1589	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K091176	.	engineered_region	1556	1596	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K091176
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagat
ggcccgggatgatcctcacttcaattttcgtatgccaatggaagtaagagagaaattgaaatttagagcagaggcaaac
ggacggagcatgaactctgagcttttgcaaatcgtacaagatgccctaagcaaaccgtcaccagtcactgggtaccgca
atgatgcggaacgactcgccgatgagcagagcgagttagtgaagaagatggtcttcgatacactgaaggatctttataa
aaaaaccaccgctgcaaacgacgaaaactacgctttagtagcttaataaccctgatagtgctagtgtagatccctacta
gagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgc
tctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagctcgaggtgagtgcacag
tactaggtccacggtgacctagatctcctatagttccctatcagtgatagagaactgtaatccctatcagtgatagaga
ttactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaa
ttagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttaccc
ttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatg
ctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaaga
actatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaa
tcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaa
tgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagc
gttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgt
ccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgg
gattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaa
agactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtggg
cctttctgcgtttata