LOCUS BBa_K091177 1968 bp DNA linear 22-Dec-2024 DEFINITION pMnt/tetR-RBS-GFP-TT-pBAD-RBS-tetR-TT ACCESSION BBa_K091177 VERSION BBa_K091177.1 FEATURES Location/Qualifiers promoter 47..52 /label=-10 misc_feature 53..71 /label=tetR1 misc_feature 78..98 /label=tetR2 misc_feature 1..52 /label=Mnt promoter 1..98 /label=BBa_K091105 RBS 107..119 /label=RBS-3\Weak RBS 113..116 /label=RBS misc_feature 107..119 /label=BBa_B0032 RBS 107..119 /label=BBa_B0032 CDS 126..845 /label=GFP protein CDS 126..845 /label=BBa_E0040 stem_loop 865..908 /label=stem_loop misc_feature 854..933 /label=BBa_B0010 terminator 854..933 /label=BBa_B0010 stem_loop 949..968 /label=T7 TE misc_feature 975..975 /label=stop polyA_site 969..982 /label=polya misc_feature 942..982 /label=BBa_B0012 terminator 942..982 /label=BBa_B0012 misc_feature 1..982 /label=BBa_K091160 promoter 991..1120 /label=BBa_I13453 misc_feature 1133..1136 /label=conserved RBS 1129..1140 /label=BBa_B0034 CDS 1150..1766 /label=tetR misc_feature 1767..1800 /label=SsrA misc_feature 1807..1831 /label=Help:Barcodes CDS 1147..1806 /label=BBa_C0040 misc_feature 1129..1831 /label=BBa_S0101 misc_feature 991..1831 /label=BBa_K091133 stem_loop 1851..1894 /label=stem_loop misc_feature 1840..1919 /label=BBa_B0010 terminator 1840..1919 /label=BBa_B0010 stem_loop 1935..1954 /label=T7 TE misc_feature 1961..1961 /label=stop polyA_site 1955..1968 /label=polya misc_feature 1928..1968 /label=BBa_B0012 terminator 1928..1968 /label=BBa_B0012 terminator 1840..1968 /label=BBa_B0015 misc_feature 991..1968 /label=BBa_K091145 ORIGIN 1 ctcgaggtga gtgcacagta ctaggtccac ggtgacctag atctcctata gttccctatc 61 agtgatagag aactgtaatc cctatcagtg atagagatta ctagagtcac acaggaaagt 121 actagatgcg taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag 181 atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat 241 acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa 301 cacttgtcac tactttcggt tatggtgttc aatgctttgc gagataccca gatcatatga 361 aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat 421 ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc 481 ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac 541 acaaattgga atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga 601 atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc gttcaactag 661 cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc 721 attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg 781 tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaat 841 aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt 901 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg 961 ggtgggcctt tctgcgttta tatactagag acattgatta tttgcacggc gtcacacttt 1021 gctatgccat agcattttta tccataagat tagcggatcc tacctgacgc tttttatcgc 1081 aactctctac tgtttctcca taccgttttt ttgggctagc tactagagaa agaggagaaa 1141 tactagatgt ccagattaga taaaagtaaa gtgattaaca gcgcattaga gctgcttaat 1201 gaggtcggaa tcgaaggttt aacaacccgt aaactcgccc agaagctagg tgtagagcag 1261 cctacattgt attggcatgt aaaaaataag cgggctttgc tcgacgcctt agccattgag 1321 atgttagata ggcaccatac tcacttttgc cctttagaag gggaaagctg gcaagatttt 1381 ttacgtaata acgctaaaag ttttagatgt gctttactaa gtcatcgcga tggagcaaaa 1441 gtacatttag gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc 1501 tttttatgcc aacaaggttt ttcactagag aatgcattat atgcactcag cgctgtgggg 1561 cattttactt taggttgcgt attggaagat caagagcatc aagtcgctaa agaagaaagg 1621 gaaacaccta ctactgatag tatgccgcca ttattacgac aagctatcga attatttgat 1681 caccaaggtg cagagccagc cttcttattc ggccttgaat tgatcatatg cggattagaa 1741 aaacaactta aatgtgaaag tgggtccgct gcaaacgacg aaaactacgc tttagtagct 1801 taataacact gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa 1861 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 1921 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata //