##gff-version 3
##sequence-region BBa_K091177 1 1968
BBa_K091177	.	engineered_region	991	1968	.	+	0	ID=BBa_K091145;Name=BBa_K091145
BBa_K091177	.	terminator	1840	1968	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K091145
BBa_K091177	.	terminator	1928	1968	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K091177	.	stem_loop	1935	1954	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K091177	.	stop_codon	1961	1961	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K091177	.	polyA_site	1955	1968	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K091177	.	engineered_region	1928	1968	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K091177	.	terminator	1840	1919	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K091177	.	stem_loop	1851	1894	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K091177	.	engineered_region	1840	1919	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K091177	.	engineered_region	991	1831	.	+	0	ID=BBa_K091133;Name=BBa_K091133;Parent=BBa_K091145
BBa_K091177	.	promoter	991	1120	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K091133
BBa_K091177	.	engineered_region	1129	1831	.	+	0	ID=BBa_S0101;Name=BBa_S0101;Parent=BBa_K091133
BBa_K091177	.	ribosome_entry_site	1129	1140	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S0101
BBa_K091177	.	conserved_region	1133	1136	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K091177	.	CDS	1147	1806	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_S0101
BBa_K091177	.	tag	1767	1800	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K091177	.	engineered_tag	1807	1831	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K091177	.	CDS	1150	1766	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K091177	.	engineered_region	1	982	.	+	0	ID=BBa_K091160;Name=BBa_K091160
BBa_K091177	.	CDS	126	845	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_K091160
BBa_K091177	.	CDS	126	845	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K091177	.	ribosome_entry_site	107	119	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K091160
BBa_K091177	.	engineered_region	107	119	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K091177	.	ribosome_entry_site	113	116	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K091177	.	ribosome_entry_site	107	119	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K091177	.	promoter	1	98	.	+	0	ID=BBa_K091105;Name=BBa_K091105;Parent=BBa_K091160
BBa_K091177	.	operator	1	52	.	+	0	ID=annotation1963742;Name=Mnt;Parent=BBa_K091105
BBa_K091177	.	operator	78	98	.	+	0	ID=annotation1963741;Name=tetR2;Parent=BBa_K091105
BBa_K091177	.	promoter	47	52	.	+	0	ID=annotation1963739;Name=-10;Parent=BBa_K091105
BBa_K091177	.	operator	53	71	.	+	0	ID=annotation1963740;Name=tetR1;Parent=BBa_K091105
BBa_K091177	.	terminator	942	982	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K091160
BBa_K091177	.	stem_loop	949	968	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K091177	.	stop_codon	975	975	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K091177	.	polyA_site	969	982	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K091177	.	engineered_region	942	982	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K091177	.	terminator	854	933	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K091160
BBa_K091177	.	stem_loop	865	908	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K091177	.	engineered_region	854	933	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_K091177
ctcgaggtgagtgcacagtactaggtccacggtgacctagatctcctatagttccctatcagtgatagagaactgtaat
ccctatcagtgatagagattactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagtt
gtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaa
catacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactacttt
cggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaa
ggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtg
atacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaata
caactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacac
aacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttac
cagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttga
gtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaa
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacact
ggctcaccttcgggtgggcctttctgcgtttatatactagagacattgattatttgcacggcgtcacactttgctatgc
catagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgt
ttttttgggctagctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgca
ttagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagccta
cattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactca
cttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagt
catcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcct
ttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgt
attggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacga
caagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattag
aaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtg
ctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatct
gttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata