LOCUS       BBa_K091178             1596 bp    DNA     linear       22-Dec-2024
DEFINITION  pMnt/tetR-RBS-GFP-TT-pBAD-RBS-Mnt-TT
ACCESSION   BBa_K091178
VERSION     BBa_K091178.1  
FEATURES             Location/Qualifiers
     promoter        47..52
                     /label=-10
     misc_feature    53..71
                     /label=tetR1
     misc_feature    78..98
                     /label=tetR2
     misc_feature    1..52
                     /label=Mnt
     promoter        1..98
                     /label=BBa_K091105
     RBS             107..119
                     /label=RBS-3\Weak
     RBS             113..116
                     /label=RBS
     misc_feature    107..119
                     /label=BBa_B0032
     RBS             107..119
                     /label=BBa_B0032
     CDS             126..845
                     /label=GFP protein
     CDS             126..845
                     /label=BBa_E0040
     stem_loop       865..908
                     /label=stem_loop
     misc_feature    854..933
                     /label=BBa_B0010
     terminator      854..933
                     /label=BBa_B0010
     stem_loop       949..968
                     /label=T7 TE
     misc_feature    975..975
                     /label=stop
     polyA_site      969..982
                     /label=polya
     misc_feature    942..982
                     /label=BBa_B0012
     terminator      942..982
                     /label=BBa_B0012
     misc_feature    1..982
                     /label=BBa_K091160
     promoter        991..1120
                     /label=BBa_I13453
     misc_feature    1133..1136
                     /label=conserved
     RBS             1129..1140
                     /label=BBa_B0034
     CDS             1147..1395
                     /label=mnt
     misc_feature    1241..1243
                     /label=T
     misc_feature    1396..1428
                     /label=LVA
     misc_feature    1435..1459
                     /label=Help:Barcodes
     CDS             1147..1434
                     /label=BBa_C0072
     misc_feature    1129..1459
                     /label=BBa_I13025
     stem_loop       1479..1522
                     /label=stem_loop
     misc_feature    1468..1547
                     /label=BBa_B0010
     terminator      1468..1547
                     /label=BBa_B0010
     stem_loop       1563..1582
                     /label=T7 TE
     misc_feature    1589..1589
                     /label=stop
     polyA_site      1583..1596
                     /label=polya
     misc_feature    1556..1596
                     /label=BBa_B0012
     terminator      1556..1596
                     /label=BBa_B0012
     terminator      1468..1596
                     /label=BBa_B0015
     misc_feature    1129..1596
                     /label=BBa_K091137
     misc_feature    991..1596
                     /label=BBa_K091147
ORIGIN
        1 ctcgaggtga gtgcacagta ctaggtccac ggtgacctag atctcctata gttccctatc
       61 agtgatagag aactgtaatc cctatcagtg atagagatta ctagagtcac acaggaaagt
      121 actagatgcg taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag
      181 atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat
      241 acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa
      301 cacttgtcac tactttcggt tatggtgttc aatgctttgc gagataccca gatcatatga
      361 aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat
      421 ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc
      481 ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac
      541 acaaattgga atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga
      601 atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc gttcaactag
      661 cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc
      721 attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg
      781 tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaat
      841 aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
      901 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
      961 ggtgggcctt tctgcgttta tatactagag acattgatta tttgcacggc gtcacacttt
     1021 gctatgccat agcattttta tccataagat tagcggatcc tacctgacgc tttttatcgc
     1081 aactctctac tgtttctcca taccgttttt ttgggctagc tactagagaa agaggagaaa
     1141 tactagatgg cccgggatga tcctcacttc aattttcgta tgccaatgga agtaagagag
     1201 aaattgaaat ttagagcaga ggcaaacgga cggagcatga actctgagct tttgcaaatc
     1261 gtacaagatg ccctaagcaa accgtcacca gtcactgggt accgcaatga tgcggaacga
     1321 ctcgccgatg agcagagcga gttagtgaag aagatggtct tcgatacact gaaggatctt
     1381 tataaaaaaa ccaccgctgc aaacgacgaa aactacgctt tagtagctta ataaccctga
     1441 tagtgctagt gtagatccct actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     1501 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     1561 ctggctcacc ttcgggtggg cctttctgcg tttata
//