##gff-version 3
##sequence-region BBa_K091178 1 1596
BBa_K091178	.	engineered_region	991	1596	.	+	0	ID=BBa_K091147;Name=BBa_K091147
BBa_K091178	.	engineered_region	1129	1596	.	+	0	ID=BBa_K091137;Name=BBa_K091137;Parent=BBa_K091147
BBa_K091178	.	terminator	1468	1596	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K091137
BBa_K091178	.	terminator	1556	1596	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K091178	.	polyA_site	1583	1596	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K091178	.	engineered_region	1556	1596	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K091178	.	stem_loop	1563	1582	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K091178	.	stop_codon	1589	1589	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K091178	.	terminator	1468	1547	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K091178	.	stem_loop	1479	1522	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K091178	.	engineered_region	1468	1547	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K091178	.	engineered_region	1129	1459	.	+	0	ID=BBa_I13025;Name=BBa_I13025;Parent=BBa_K091137
BBa_K091178	.	CDS	1147	1434	.	+	0	ID=BBa_C0072;Name=BBa_C0072;Parent=BBa_I13025
BBa_K091178	.	point_mutation	1241	1243	.	+	0	ID=annotation308502;Name=T;Parent=BBa_C0072
BBa_K091178	.	CDS	1147	1395	.	+	0	ID=annotation308499;Name=mnt;Parent=BBa_C0072
BBa_K091178	.	engineered_tag	1435	1459	.	+	0	ID=annotation2214010;Name=Help:Barcodes;Parent=BBa_C0072
BBa_K091178	.	tag	1396	1428	.	+	0	ID=annotation308503;Name=LVA;Parent=BBa_C0072
BBa_K091178	.	ribosome_entry_site	1129	1140	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13025
BBa_K091178	.	conserved_region	1133	1136	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K091178	.	promoter	991	1120	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K091147
BBa_K091178	.	engineered_region	1	982	.	+	0	ID=BBa_K091160;Name=BBa_K091160
BBa_K091178	.	CDS	126	845	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_K091160
BBa_K091178	.	CDS	126	845	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K091178	.	ribosome_entry_site	107	119	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K091160
BBa_K091178	.	engineered_region	107	119	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K091178	.	ribosome_entry_site	107	119	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K091178	.	ribosome_entry_site	113	116	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K091178	.	terminator	942	982	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K091160
BBa_K091178	.	polyA_site	969	982	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K091178	.	engineered_region	942	982	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K091178	.	stem_loop	949	968	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K091178	.	stop_codon	975	975	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K091178	.	terminator	854	933	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K091160
BBa_K091178	.	stem_loop	865	908	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K091178	.	engineered_region	854	933	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K091178	.	promoter	1	98	.	+	0	ID=BBa_K091105;Name=BBa_K091105;Parent=BBa_K091160
BBa_K091178	.	operator	1	52	.	+	0	ID=annotation1963742;Name=Mnt;Parent=BBa_K091105
BBa_K091178	.	operator	78	98	.	+	0	ID=annotation1963741;Name=tetR2;Parent=BBa_K091105
BBa_K091178	.	operator	53	71	.	+	0	ID=annotation1963740;Name=tetR1;Parent=BBa_K091105
BBa_K091178	.	promoter	47	52	.	+	0	ID=annotation1963739;Name=-10;Parent=BBa_K091105
>BBa_K091178
ctcgaggtgagtgcacagtactaggtccacggtgacctagatctcctatagttccctatcagtgatagagaactgtaat
ccctatcagtgatagagattactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagtt
gtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaa
catacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactacttt
cggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaa
ggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtg
atacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaata
caactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacac
aacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttac
cagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttga
gtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaa
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacact
ggctcaccttcgggtgggcctttctgcgtttatatactagagacattgattatttgcacggcgtcacactttgctatgc
catagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgt
ttttttgggctagctactagagaaagaggagaaatactagatggcccgggatgatcctcacttcaattttcgtatgcca
atggaagtaagagagaaattgaaatttagagcagaggcaaacggacggagcatgaactctgagcttttgcaaatcgtac
aagatgccctaagcaaaccgtcaccagtcactgggtaccgcaatgatgcggaacgactcgccgatgagcagagcgagtt
agtgaagaagatggtcttcgatacactgaaggatctttataaaaaaaccaccgctgcaaacgacgaaaactacgcttta
gtagcttaataaccctgatagtgctagtgtagatccctactagagccaggcatcaaataaaacgaaaggctcagtcgaa
agactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtggg
cctttctgcgtttata