BBa_K091178
type: ComponentDefinition
persistent identity: BBa K091178
display id: BBa_K091178
version: 1
was generated by: iGEM to SBOL conversion
description: pMnt/tetR-RBS-GFP-TT-pBAD-RBS-Mnt-TT
created: 2008-08-03T11:00:00Z
modified: 2015-05-08T01:08:38Z
mutable provenance: Both of these parts contains parts from the registry. K091160 contains DNA built from oligos.
owned by: james
owned by: myers
mutable description: This part was made by ligating K091160 to the 5' end of K091147.
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: no special considerations
star: false
creator: Robert Cool
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K091160
component: BBa_K091147
sequence annotation: BBa_K091160
sequence annotation: BBa_K091147
sequence: https://synbiohub.org/public/igem/BBa_K091178_sequence/1

BBa_K091160
type: Component
persistent identity: component2281561
display id: component2281561
version: 1
top level: BBa_K091178
definition: BBa_K091160
access: public

BBa_K091147
type: Component
persistent identity: component2281579
display id: component2281579
version: 1
top level: BBa_K091178
definition: BBa_K091147
access: public

BBa_K091160
type: SequenceAnnotation
persistent identity: annotation2281561
display id: annotation2281561
version: 1
top level: BBa_K091178
location: https://synbiohub.org/public/igem/BBa_K091178/annotation2281561/range2281561/1
component: BBa_K091160

https://synbiohub.org/public/igem/BBa_K091178/annotation2281561/range2281561/1
type: Range
persistent identity: range2281561
display id: range2281561
version: 1
top level: BBa_K091178
direction: direction/reverse
start: 1
end: 982
orientation: inline

BBa_K091147
type: SequenceAnnotation
persistent identity: annotation2281579
display id: annotation2281579
version: 1
top level: BBa_K091178
location: https://synbiohub.org/public/igem/BBa_K091178/annotation2281579/range2281579/1
component: BBa_K091147

https://synbiohub.org/public/igem/BBa_K091178/annotation2281579/range2281579/1
type: Range
persistent identity: range2281579
display id: range2281579
version: 1
top level: BBa_K091178
direction: direction/reverse
start: 991
end: 1596
orientation: inline

BBa_K091147
type: ComponentDefinition
persistent identity: BBa K091147
display id: BBa_K091147
version: 1
was generated by: iGEM to SBOL conversion
description: pBAD+RBS+Mnt+TT
created: 2008-07-16T11:00:00Z
modified: 2015-05-08T01:08:38Z
mutable provenance: I13453 is found in the registry, K091137 is a composite part built at Missouri Western.
owned by: james
owned by: myers
mutable description: This part was made by ligating I13453 to K091137.
discontinued: false
dominant: true
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: no special considerations
star: false
creator: Alicia Allen
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K091137
component: BBa_I13453
sequence annotation: BBa_I13453
sequence annotation: BBa_K091137
sequence: https://synbiohub.org/public/igem/BBa_K091147_sequence/1

BBa_K091137
type: Component
persistent identity: component2254213
display id: component2254213
version: 1
top level: BBa_K091147
definition: BBa_K091137
access: public

BBa_I13453
type: Component
persistent identity: component2254197
display id: component2254197
version: 1
top level: BBa_K091147
definition: BBa_I13453
access: public

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2254197
display id: annotation2254197
version: 1
top level: BBa_K091147
location: https://synbiohub.org/public/igem/BBa_K091147/annotation2254197/range2254197/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K091147/annotation2254197/range2254197/1
type: Range
persistent identity: range2254197
display id: range2254197
version: 1
top level: BBa_K091147
direction: direction/reverse
start: 1
end: 130
orientation: inline

BBa_K091137
type: SequenceAnnotation
persistent identity: annotation2254213
display id: annotation2254213
version: 1
top level: BBa_K091147
location: https://synbiohub.org/public/igem/BBa_K091147/annotation2254213/range2254213/1
component: BBa_K091137

https://synbiohub.org/public/igem/BBa_K091147/annotation2254213/range2254213/1
type: Range
persistent identity: range2254213
display id: range2254213
version: 1
top level: BBa_K091147
direction: direction/reverse
start: 139
end: 606
orientation: inline

BBa_K091160
type: ComponentDefinition
persistent identity: BBa K091160
display id: BBa_K091160
version: 1
was generated by: iGEM to SBOL conversion
description: pMnt/Tet-RBS-GFP-TT
created: 2008-07-27T11:00:00Z
modified: 2015-05-08T01:08:38Z
mutable provenance: E0240 can be found in the registry, K091105 was built from oligos.
owned by: james
owned by: myers
mutable description: This part was made by ligating K091105 with E0240.
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: no special considerations
star: false
creator: Robert Cool
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E0040
component: BBa_B0032
component: BBa_K091105
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_K091105
sequence annotation: BBa_B0032
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K091160_sequence/1

BBa_E0040
type: Component
persistent identity: component1968926
display id: component1968926
version: 1
top level: BBa_K091160
definition: GFP
access: public

BBa_B0032
type: Component
persistent identity: component1968923
display id: component1968923
version: 1
top level: BBa_K091160
definition: BBa_B0032
access: public

BBa_K091105
type: Component
persistent identity: component1968921
display id: component1968921
version: 1
top level: BBa_K091160
definition: BBa_K091105
access: public

BBa_B0012
type: Component
persistent identity: component1968929
display id: component1968929
version: 1
top level: BBa_K091160
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1968927
display id: component1968927
version: 1
top level: BBa_K091160
definition: BBa_B0010
access: public

BBa_K091105
type: SequenceAnnotation
persistent identity: annotation1968921
display id: annotation1968921
version: 1
top level: BBa_K091160
location: https://synbiohub.org/public/igem/BBa_K091160/annotation1968921/range1968921/1
component: BBa_K091105

https://synbiohub.org/public/igem/BBa_K091160/annotation1968921/range1968921/1
type: Range
persistent identity: range1968921
display id: range1968921
version: 1
top level: BBa_K091160
direction: direction/reverse
start: 1
end: 98
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1968923
display id: annotation1968923
version: 1
top level: BBa_K091160
location: https://synbiohub.org/public/igem/BBa_K091160/annotation1968923/range1968923/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K091160/annotation1968923/range1968923/1
type: Range
persistent identity: range1968923
display id: range1968923
version: 1
top level: BBa_K091160
direction: direction/forward
start: 107
end: 119
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1968926
display id: annotation1968926
version: 1
top level: BBa_K091160
location: https://synbiohub.org/public/igem/BBa_K091160/annotation1968926/range1968926/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K091160/annotation1968926/range1968926/1
type: Range
persistent identity: range1968926
display id: range1968926
version: 1
top level: BBa_K091160
direction: direction/reverse
start: 126
end: 845
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1968927
display id: annotation1968927
version: 1
top level: BBa_K091160
location: https://synbiohub.org/public/igem/BBa_K091160/annotation1968927/range1968927/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K091160/annotation1968927/range1968927/1
type: Range
persistent identity: range1968927
display id: range1968927
version: 1
top level: BBa_K091160
direction: direction/forward
start: 854
end: 933
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1968929
display id: annotation1968929
version: 1
top level: BBa_K091160
location: https://synbiohub.org/public/igem/BBa_K091160/annotation1968929/range1968929/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K091160/annotation1968929/range1968929/1
type: Range
persistent identity: range1968929
display id: range1968929
version: 1
top level: BBa_K091160
direction: direction/forward
start: 942
end: 982
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_I13453
type: ComponentDefinition
persistent identity: BBa I13453
display id: BBa_I13453
version: 1
was generated by: iGEM to SBOL conversion
description: Pbad promoter
created: 2005-05-24T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: PBad promoter from I0500 without AraC.
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I13453_sequence/1

BBa_K091105
type: ComponentDefinition
persistent identity: BBa K091105
display id: BBa_K091105
version: 1
was generated by: iGEM to SBOL conversion
description: pTet/Mnt Hybrid Promoter
created: 2008-06-18T11:00:00Z
modified: 2015-05-08T01:08:37Z
mutable provenance: This part comes from the Mnt promoter and the TetR promoter
owned by: james
owned by: myers
mutable description: This promoter is a modified version of the Mnt promoter that is also responsive to TetR. The promoter should be repressed by Mnt repressor. It should also be repressed by TetR, and in the absence of Mnt repressor, should be induced by aTc.
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Mnt repressor binds as a tetramer to two half-operator sites. Introduction of TetR binding sites between the Mnt promoter -10 sequence and the start site for transcription should allow for repression by TetR. Accordingly, the hybrid promoter was designed by 1) Remove all bases of mnt promoter 3??? to -10: gagtcgtattaattt will be replaced by tccctatcagtgata, and 2) truncate the second half of ptet -10 and mutate -35 so that it function to bind tetR (replace ttgaca with actgta), but is not a promoter, and 3) place the tetR1 and tetR2 binding sites 3??? to truncated mnt -10 sequence.
star: true
creator: Robert Cool
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Mnt
sequence annotation: -10
sequence annotation: tetR1
sequence annotation: tetR2
sequence: https://synbiohub.org/public/igem/BBa_K091105_sequence/1

Mnt
type: SequenceAnnotation
persistent identity: annotation1963742
display id: annotation1963742
version: 1
top level: BBa_K091105
location: https://synbiohub.org/public/igem/BBa_K091105/annotation1963742/range1963742/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K091105/annotation1963742/range1963742/1
type: Range
persistent identity: range1963742
display id: range1963742
version: 1
top level: BBa_K091105
direction: direction/forward
start: 1
end: 52
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1963739
display id: annotation1963739
version: 1
top level: BBa_K091105
location: https://synbiohub.org/public/igem/BBa_K091105/annotation1963739/range1963739/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K091105/annotation1963739/range1963739/1
type: Range
persistent identity: range1963739
display id: range1963739
version: 1
top level: BBa_K091105
direction: direction/forward
start: 47
end: 52
orientation: inline

tetR1
type: SequenceAnnotation
persistent identity: annotation1963740
display id: annotation1963740
version: 1
top level: BBa_K091105
location: https://synbiohub.org/public/igem/BBa_K091105/annotation1963740/range1963740/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K091105/annotation1963740/range1963740/1
type: Range
persistent identity: range1963740
display id: range1963740
version: 1
top level: BBa_K091105
direction: direction/forward
start: 53
end: 71
orientation: inline

tetR2
type: SequenceAnnotation
persistent identity: annotation1963741
display id: annotation1963741
version: 1
top level: BBa_K091105
location: https://synbiohub.org/public/igem/BBa_K091105/annotation1963741/range1963741/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K091105/annotation1963741/range1963741/1
type: Range
persistent identity: range1963741
display id: range1963741
version: 1
top level: BBa_K091105
direction: direction/forward
start: 78
end: 98
orientation: inline

BBa_K091137
type: ComponentDefinition
persistent identity: BBa K091137
display id: BBa_K091137
version: 1
was generated by: iGEM to SBOL conversion
description: RBS+Mnt+TT
created: 2008-07-13T11:00:00Z
modified: 2015-05-08T01:08:38Z
mutable provenance: These parts can be found in the registry.
owned by: james
owned by: myers
mutable description: This part was made by ligating I13025 to B0015.
discontinued: false
dominant: true
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: no special cosiderations
star: false
creator: Robert Cool
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0015
component: BBa_I13025
sequence annotation: BBa_I13025
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K091137_sequence/1

BBa_B0015
type: Component
persistent identity: component2249118
display id: component2249118
version: 1
top level: BBa_K091137
definition: BBa_B0015
access: public

BBa_I13025
type: Component
persistent identity: component2249111
display id: component2249111
version: 1
top level: BBa_K091137
definition: BBa_I13025
access: public

BBa_I13025
type: SequenceAnnotation
persistent identity: annotation2249111
display id: annotation2249111
version: 1
top level: BBa_K091137
location: https://synbiohub.org/public/igem/BBa_K091137/annotation2249111/range2249111/1
component: BBa_I13025

https://synbiohub.org/public/igem/BBa_K091137/annotation2249111/range2249111/1
type: Range
persistent identity: range2249111
display id: range2249111
version: 1
top level: BBa_K091137
direction: direction/reverse
start: 1
end: 331
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2249118
display id: annotation2249118
version: 1
top level: BBa_K091137
location: https://synbiohub.org/public/igem/BBa_K091137/annotation2249118/range2249118/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K091137/annotation2249118/range2249118/1
type: Range
persistent identity: range2249118
display id: range2249118
version: 1
top level: BBa_K091137
direction: direction/reverse
start: 340
end: 468
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_I13025
type: ComponentDefinition
persistent identity: BBa I13025
display id: BBa_I13025
version: 1
was generated by: iGEM to SBOL conversion
description: mnt QPI Intermediate
created: 2004-07-14T11:00:00Z
modified: 2015-08-31T04:07:32Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: jenmitch
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_C0072
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0072
sequence: https://synbiohub.org/public/igem/BBa_I13025_sequence/1

BBa_C0072
type: Component
persistent identity: component939424
display id: component939424
version: 1
top level: BBa_I13025
definition: mnt (s)
access: public

BBa_B0034
type: Component
persistent identity: component939411
display id: component939411
version: 1
top level: BBa_I13025
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation939411
display id: annotation939411
version: 1
top level: BBa_I13025
location: https://synbiohub.org/public/igem/BBa_I13025/annotation939411/range939411/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13025/annotation939411/range939411/1
type: Range
persistent identity: range939411
display id: range939411
version: 1
top level: BBa_I13025
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0072
type: SequenceAnnotation
persistent identity: annotation939424
display id: annotation939424
version: 1
top level: BBa_I13025
location: https://synbiohub.org/public/igem/BBa_I13025/annotation939424/range939424/1
component: BBa_C0072

https://synbiohub.org/public/igem/BBa_I13025/annotation939424/range939424/1
type: Range
persistent identity: range939424
display id: range939424
version: 1
top level: BBa_I13025
direction: direction/forward
start: 19
end: 306
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

mnt (s)
type: ComponentDefinition
persistent identity: BBa C0072
display id: BBa_C0072
version: 1
was generated by: iGEM to SBOL conversion
description: mnt repressor (strong) from Salmonella phage P22 (+LVA)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: enterobacteriophage p22
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: wild type dimeric repressor from enterobacteriophage p22 with dissociation constant of 2.5*10^-12. sequence was translated with the E.Coli K12 codon usage table, and nucleotides 95-97 was changed from AAT to AAC to avoid the cut site GAATTC (94-99). Identification of Functionally Important Residues in the DNA Binding Region of the MNT Repressor Kendall, KL; Sauer, RT The Journal of Biological Chemistry, 264(23):13706-13710, 1989
star: true
creator: crackdots
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mnt
sequence annotation: T
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0072_sequence/1

mnt
type: SequenceAnnotation
persistent identity: annotation308499
display id: annotation308499
version: 1
top level: mnt (s)
location: https://synbiohub.org/public/igem/BBa_C0072/annotation308499/range308499/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0072/annotation308499/range308499/1
type: Range
persistent identity: range308499
display id: range308499
version: 1
top level: mnt (s)
direction: direction/forward
start: 1
end: 249
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation308502
display id: annotation308502
version: 1
top level: mnt (s)
location: https://synbiohub.org/public/igem/BBa_C0072/annotation308502/range308502/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0072/annotation308502/range308502/1
type: Range
persistent identity: range308502
display id: range308502
version: 1
top level: mnt (s)
direction: direction/forward
start: 95
end: 97
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation308503
display id: annotation308503
version: 1
top level: mnt (s)
location: https://synbiohub.org/public/igem/BBa_C0072/annotation308503/range308503/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0072/annotation308503/range308503/1
type: Range
persistent identity: range308503
display id: range308503
version: 1
top level: mnt (s)
direction: direction/forward
start: 250
end: 282
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214010
display id: annotation2214010
version: 1
top level: mnt (s)
location: https://synbiohub.org/public/igem/BBa_C0072/annotation2214010/range2214010/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0072/annotation2214010/range2214010/1
type: Range
persistent identity: range2214010
display id: range2214010
version: 1
top level: mnt (s)
direction: direction/forward
start: 289
end: 313
orientation: inline

https://synbiohub.org/public/igem/BBa_K091178_sequence/1
type: Sequence
persistent identity: BBa K091178 sequence
display id: BBa_K091178_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcgaggtgagtgcacagtactaggtccacggtgacctagatctcctatagttccctatcagtgatagagaactgtaatccctatcagtgatagagattactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatggcccgggatgatcctcacttcaattttcgtatgccaatggaagtaagagagaaattgaaatttagagcagaggcaaacggacggagcatgaactctgagcttttgcaaatcgtacaagatgccctaagcaaaccgtcaccagtcactgggtaccgcaatgatgcggaacgactcgccgatgagcagagcgagttagtgaagaagatggtcttcgatacactgaaggatctttataaaaaaaccaccgctgcaaacgacgaaaactacgctttagtagcttaataaccctgatagtgctagtgtagatccctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K091147_sequence/1
type: Sequence
persistent identity: BBa K091147 sequence
display id: BBa_K091147_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatggcccgggatgatcctcacttcaattttcgtatgccaatggaagtaagagagaaattgaaatttagagcagaggcaaacggacggagcatgaactctgagcttttgcaaatcgtacaagatgccctaagcaaaccgtcaccagtcactgggtaccgcaatgatgcggaacgactcgccgatgagcagagcgagttagtgaagaagatggtcttcgatacactgaaggatctttataaaaaaaccaccgctgcaaacgacgaaaactacgctttagtagcttaataaccctgatagtgctagtgtagatccctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K091160_sequence/1
type: Sequence
persistent identity: BBa K091160 sequence
display id: BBa_K091160_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcgaggtgagtgcacagtactaggtccacggtgacctagatctcctatagttccctatcagtgatagagaactgtaatccctatcagtgatagagattactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13453_sequence/1
type: Sequence
persistent identity: BBa I13453 sequence
display id: BBa_I13453_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K091105_sequence/1
type: Sequence
persistent identity: BBa K091105 sequence
display id: BBa_K091105_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcgaggtgagtgcacagtactaggtccacggtgacctagatctcctatagttccctatcagtgatagagaactgtaatccctatcagtgatagagat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K091137_sequence/1
type: Sequence
persistent identity: BBa K091137 sequence
display id: BBa_K091137_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggcccgggatgatcctcacttcaattttcgtatgccaatggaagtaagagagaaattgaaatttagagcagaggcaaacggacggagcatgaactctgagcttttgcaaatcgtacaagatgccctaagcaaaccgtcaccagtcactgggtaccgcaatgatgcggaacgactcgccgatgagcagagcgagttagtgaagaagatggtcttcgatacactgaaggatctttataaaaaaaccaccgctgcaaacgacgaaaactacgctttagtagcttaataaccctgatagtgctagtgtagatccctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13025_sequence/1
type: Sequence
persistent identity: BBa I13025 sequence
display id: BBa_I13025_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggcccgggatgatcctcacttcaattttcgtatgccaatggaagtaagagagaaattgaaatttagagcagaggcaaacggacggagcatgaactctgagcttttgcaaatcgtacaagatgccctaagcaaaccgtcaccagtcactgggtaccgcaatgatgcggaacgactcgccgatgagcagagcgagttagtgaagaagatggtcttcgatacactgaaggatctttataaaaaaaccaccgctgcaaacgacgaaaactacgctttagtagcttaataaccctgatagtgctagtgtagatccc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0072_sequence/1
type: Sequence
persistent identity: BBa C0072 sequence
display id: BBa_C0072_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcccgggatgatcctcacttcaattttcgtatgccaatggaagtaagagagaaattgaaatttagagcagaggcaaacggacggagcatgaactctgagcttttgcaaatcgtacaagatgccctaagcaaaccgtcaccagtcactgggtaccgcaatgatgcggaacgactcgccgatgagcagagcgagttagtgaagaagatggtcttcgatacactgaaggatctttataaaaaaaccaccgctgcaaacgacgaaaactacgctttagtagcttaataaccctgatagtgctagtgtagatccc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z