LOCUS       BBa_K091180              999 bp    DNA     linear       22-Dec-2024
DEFINITION  pLac-RBS-LasR-TT
ACCESSION   BBa_K091180
VERSION     BBa_K091180.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..798
                     /label=lasR
     misc_feature    799..831
                     /label=LVA
     misc_feature    561..561
                     /label=G
     misc_feature    838..862
                     /label=Help:Barcodes
     CDS             82..837
                     /label=BBa_C0079
     misc_feature    64..862
                     /label=BBa_S03156
     stem_loop       882..925
                     /label=stem_loop
     misc_feature    871..950
                     /label=BBa_B0010
     terminator      871..950
                     /label=BBa_B0010
     stem_loop       966..985
                     /label=T7 TE
     misc_feature    992..992
                     /label=stop
     polyA_site      986..999
                     /label=polya
     misc_feature    959..999
                     /label=BBa_B0012
     terminator      959..999
                     /label=BBa_B0012
     terminator      871..999
                     /label=BBa_B0015
     misc_feature    64..999
                     /label=BBa_S03954
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatggccttg gttgacggtt ttcttgagct ggaacgctca
      121 agtggaaaat tggagtggag cgccatcctc cagaagatgg cgagcgacct tggattctcg
      181 aagatcctgt tcggcctgtt gcctaaggac agccaggact acgagaacgc cttcatcgtc
      241 ggcaactacc cggccgcctg gcgcgagcat tacgaccggg ctggctacgc gcgggtcgac
      301 ccgacggtca gtcactgtac ccagagcgta ctgccgattt tctgggaacc gtccatctac
      361 cagacgcgaa agcagcacga gttcttcgag gaagcctcgg ccgccggcct ggtgtatggg
      421 ctgaccatgc cgctgcatgg tgctcgcggc gaactcggcg cgctgagcct cagcgtggaa
      481 gcggaaaacc gggccgaggc caaccgtttc atagagtcgg tcctgccgac cctgtggatg
      541 ctcaaggact acgcactgca aagcggtgcc ggactggcct tcgaacatcc ggtcagcaaa
      601 ccggtggttc tgaccagccg ggagaaggaa gtgttgcagt ggtgcgccat cggcaagacc
      661 agttgggaga tatcggttat ctgcaactgc tcggaagcca atgtgaactt ccatatggga
      721 aatattcggc ggaagttcgg tgtgacctcc cgccgcgtag cggccattat ggccgttaat
      781 ttgggtctta ttactctcgc tgcaaacgac gaaaactacg ctttagtagc ttaataacac
      841 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt
      901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
      961 acactggctc accttcgggt gggcctttct gcgtttata
//