LOCUS       BBa_K091181             1985 bp    DNA     linear       11-May-2024
DEFINITION  pBad-RBS-tetR-TT-placI/cI-RBS-lasR-TT
ACCESSION   BBa_K091181
VERSION     BBa_K091181.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=BBa_I13453
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     CDS             160..776
                     /label=tetR
     misc_feature    777..810
                     /label=SsrA
     misc_feature    817..841
                     /label=Help:Barcodes
     CDS             157..816
                     /label=BBa_C0040
     misc_feature    139..841
                     /label=BBa_S0101
     misc_feature    1..841
                     /label=BBa_K091133
     stem_loop       861..904
                     /label=stem_loop
     misc_feature    850..929
                     /label=BBa_B0010
     terminator      850..929
                     /label=BBa_B0010
     stem_loop       945..964
                     /label=T7 TE
     misc_feature    971..971
                     /label=stop
     polyA_site      965..978
                     /label=polya
     misc_feature    938..978
                     /label=BBa_B0012
     terminator      938..978
                     /label=BBa_B0012
     terminator      850..978
                     /label=BBa_B0015
     misc_feature    1..978
                     /label=BBa_K091145
     misc_feature    989..1005
                     /label=lac O1
     promoter        1006..1011
                     /label=-35
     misc_feature    1012..1028
                     /label=lac O1
     promoter        1029..1034
                     /label=-10
     misc_feature    987..1040
                     /label=BBa_R0011
     promoter        987..1040
                     /label=BBa_R0011
     misc_feature    1054..1057
                     /label=conserved
     RBS             1050..1061
                     /label=BBa_B0034
     CDS             1068..1784
                     /label=lasR
     misc_feature    1785..1817
                     /label=LVA
     misc_feature    1547..1547
                     /label=G
     misc_feature    1824..1848
                     /label=Help:Barcodes
     CDS             1068..1823
                     /label=BBa_C0079
     misc_feature    1050..1848
                     /label=BBa_S03156
     stem_loop       1868..1911
                     /label=stem_loop
     misc_feature    1857..1936
                     /label=BBa_B0010
     terminator      1857..1936
                     /label=BBa_B0010
     stem_loop       1952..1971
                     /label=T7 TE
     misc_feature    1978..1978
                     /label=stop
     polyA_site      1972..1985
                     /label=polya
     misc_feature    1945..1985
                     /label=BBa_B0012
     terminator      1945..1985
                     /label=BBa_B0012
     terminator      1857..1985
                     /label=BBa_B0015
     misc_feature    1050..1985
                     /label=BBa_S03954
     misc_feature    987..1985
                     /label=BBa_S03966
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatgt ccagattaga taaaagtaaa
      181 gtgattaaca gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt
      241 aaactcgccc agaagctagg tgtagagcag cctacattgt attggcatgt aaaaaataag
      301 cgggctttgc tcgacgcctt agccattgag atgttagata ggcaccatac tcacttttgc
      361 cctttagaag gggaaagctg gcaagatttt ttacgtaata acgctaaaag ttttagatgt
      421 gctttactaa gtcatcgcga tggagcaaaa gtacatttag gtacacggcc tacagaaaaa
      481 cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt ttcactagag
      541 aatgcattat atgcactcag cgctgtgggg cattttactt taggttgcgt attggaagat
      601 caagagcatc aagtcgctaa agaagaaagg gaaacaccta ctactgatag tatgccgcca
      661 ttattacgac aagctatcga attatttgat caccaaggtg cagagccagc cttcttattc
      721 ggccttgaat tgatcatatg cggattagaa aaacaactta aatgtgaaag tgggtccgct
      781 gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta gtgtagatca
      841 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
      901 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
      961 ggcctttctg cgtttatata ctagagaatt gtgagcggat aacaattgac attgtgagcg
     1021 gataacaaga tactgagcac atactagaga aagaggagaa atactagatg gccttggttg
     1081 acggttttct tgagctggaa cgctcaagtg gaaaattgga gtggagcgcc atcctccaga
     1141 agatggcgag cgaccttgga ttctcgaaga tcctgttcgg cctgttgcct aaggacagcc
     1201 aggactacga gaacgccttc atcgtcggca actacccggc cgcctggcgc gagcattacg
     1261 accgggctgg ctacgcgcgg gtcgacccga cggtcagtca ctgtacccag agcgtactgc
     1321 cgattttctg ggaaccgtcc atctaccaga cgcgaaagca gcacgagttc ttcgaggaag
     1381 cctcggccgc cggcctggtg tatgggctga ccatgccgct gcatggtgct cgcggcgaac
     1441 tcggcgcgct gagcctcagc gtggaagcgg aaaaccgggc cgaggccaac cgtttcatag
     1501 agtcggtcct gccgaccctg tggatgctca aggactacgc actgcaaagc ggtgccggac
     1561 tggccttcga acatccggtc agcaaaccgg tggttctgac cagccgggag aaggaagtgt
     1621 tgcagtggtg cgccatcggc aagaccagtt gggagatatc ggttatctgc aactgctcgg
     1681 aagccaatgt gaacttccat atgggaaata ttcggcggaa gttcggtgtg acctcccgcc
     1741 gcgtagcggc cattatggcc gttaatttgg gtcttattac tctcgctgca aacgacgaaa
     1801 actacgcttt agtagcttaa taacactgat agtgctagtg tagatcacta ctagagccag
     1861 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     1921 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     1981 ttata
//