LOCUS BBa_K091182 2988 bp DNA linear 22-Nov-2024 DEFINITION pLasR/cI-RBS-LuxI-RBS-Mnt-TT-pMnt/tetR-RBS-LuxI-RBS-cI-TT ACCESSION BBa_K091182 VERSION BBa_K091182.1 FEATURES Location/Qualifiers promoter 140..145 /label=-10 promoter 117..122 /label=-35 misc_feature 147..164 /label=cI OR1 misc_feature 46..63 /label=plasR OP1 misc_feature 106..126 /label=plasR OP2 promoter 1..164 /label=BBa_K091143 misc_feature 177..180 /label=conserved RBS 173..184 /label=BBa_B0034 misc_feature 770..801 /label=LVA CDS 191..769 /label=luxI misc_feature 191..808 /label=BBa_C0061 misc_feature 809..833 /label=Help:Barcodes CDS 191..808 /label=BBa_C0061 misc_feature 173..833 /label=BBa_C0261 misc_feature 846..849 /label=conserved RBS 842..853 /label=BBa_B0034 CDS 860..1108 /label=mnt misc_feature 954..956 /label=T misc_feature 1109..1141 /label=LVA misc_feature 1148..1172 /label=Help:Barcodes CDS 860..1147 /label=BBa_C0072 misc_feature 842..1172 /label=BBa_I13025 stem_loop 1192..1235 /label=stem_loop misc_feature 1181..1260 /label=BBa_B0010 terminator 1181..1260 /label=BBa_B0010 stem_loop 1276..1295 /label=T7 TE misc_feature 1302..1302 /label=stop polyA_site 1296..1309 /label=polya misc_feature 1269..1309 /label=BBa_B0012 terminator 1269..1309 /label=BBa_B0012 terminator 1181..1309 /label=BBa_B0015 misc_feature 842..1309 /label=BBa_K091141 misc_feature 173..1309 /label=BBa_K091154 misc_feature 1..1309 /label=BBa_K091155 promoter 1364..1369 /label=-10 misc_feature 1370..1388 /label=tetR1 misc_feature 1395..1415 /label=tetR2 misc_feature 1318..1369 /label=Mnt promoter 1318..1415 /label=BBa_K091105 misc_feature 1428..1431 /label=conserved RBS 1424..1435 /label=BBa_B0034 misc_feature 2021..2052 /label=LVA CDS 1442..2020 /label=luxI misc_feature 1442..2059 /label=BBa_C0061 misc_feature 2060..2084 /label=Help:Barcodes CDS 1442..2059 /label=BBa_C0061 misc_feature 1424..2084 /label=BBa_C0261 misc_feature 1318..2084 /label=BBa_K091132 misc_feature 2097..2100 /label=conserved RBS 2093..2104 /label=BBa_B0034 CDS 2114..2821 /label=cI lambda misc_feature 2822..2854 /label=LVA misc_feature 2861..2885 /label=Help:Barcodes CDS 2111..2860 /label=BBa_C0051 stem_loop 2901..2920 /label=T7 TE misc_feature 2927..2927 /label=stop polyA_site 2921..2934 /label=polya misc_feature 2894..2934 /label=BBa_B0012 terminator 2894..2934 /label=BBa_B0012 stem_loop 2955..2977 /label=stem_loop misc_feature 2943..2988 /label=BBa_B0011 terminator 2943..2988 /label=BBa_B0011 misc_feature 2093..2988 /label=BBa_P0455 misc_feature 1318..2988 /label=BBa_K091144 ORIGIN 1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct 61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg 121 caggtttggc cgcgggttct ttttggtacc tctggcggtg ataatactag agaaagagga 181 gaaatactag atgactataa tgataaaaaa atcggatttt ttggcaattc catcggagga 241 gtataaaggt attctaagtc ttcgttatca agtgtttaag caaagacttg agtgggactt 301 agttgtagaa aataaccttg aatcagatga gtatgataac tcaaatgcag aatatattta 361 tgcttgtgat gatactgaaa atgtaagtgg atgctggcgt ttattaccta caacaggtga 421 ttatatgctg aaaagtgttt ttcctgaatt gcttggtcaa cagagtgctc ccaaagatcc 481 taatatagtc gaattaagtc gttttgctgt aggtaaaaat agctcaaaga taaataactc 541 tgctagtgaa attacaatga aactatttga agctatatat aaacacgctg ttagtcaagg 601 tattacagaa tatgtaacag taacatcaac agcaatagag cgatttttaa agcgtattaa 661 agttccttgt catcgtattg gagacaaaga aattcatgta ttaggtgata ctaaatcggt 721 tgtattgtct atgcctatta atgaacagtt taaaaaagca gtcttaaatg ctgcaaacga 781 cgaaaactac gctttagtag cttaataact ctgatagtgc tagtgtagat ctctactaga 841 gaaagaggag aaatactaga tggcccggga tgatcctcac ttcaattttc gtatgccaat 901 ggaagtaaga gagaaattga aatttagagc agaggcaaac ggacggagca tgaactctga 961 gcttttgcaa atcgtacaag atgccctaag caaaccgtca ccagtcactg ggtaccgcaa 1021 tgatgcggaa cgactcgccg atgagcagag cgagttagtg aagaagatgg tcttcgatac 1081 actgaaggat ctttataaaa aaaccaccgc tgcaaacgac gaaaactacg ctttagtagc 1141 ttaataaccc tgatagtgct agtgtagatc cctactagag ccaggcatca aataaaacga 1201 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 1261 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagagctc 1321 gaggtgagtg cacagtacta ggtccacggt gacctagatc tcctatagtt ccctatcagt 1381 gatagagaac tgtaatccct atcagtgata gagattacta gagaaagagg agaaatacta 1441 gatgactata atgataaaaa aatcggattt tttggcaatt ccatcggagg agtataaagg 1501 tattctaagt cttcgttatc aagtgtttaa gcaaagactt gagtgggact tagttgtaga 1561 aaataacctt gaatcagatg agtatgataa ctcaaatgca gaatatattt atgcttgtga 1621 tgatactgaa aatgtaagtg gatgctggcg tttattacct acaacaggtg attatatgct 1681 gaaaagtgtt tttcctgaat tgcttggtca acagagtgct cccaaagatc ctaatatagt 1741 cgaattaagt cgttttgctg taggtaaaaa tagctcaaag ataaataact ctgctagtga 1801 aattacaatg aaactatttg aagctatata taaacacgct gttagtcaag gtattacaga 1861 atatgtaaca gtaacatcaa cagcaataga gcgattttta aagcgtatta aagttccttg 1921 tcatcgtatt ggagacaaag aaattcatgt attaggtgat actaaatcgg ttgtattgtc 1981 tatgcctatt aatgaacagt ttaaaaaagc agtcttaaat gctgcaaacg acgaaaacta 2041 cgctttagta gcttaataac tctgatagtg ctagtgtaga tctctactag agaaagagga 2101 gaaatactag atgagcacaa aaaagaaacc attaacacaa gagcagcttg aggacgcacg 2161 tcgccttaaa gcaatttatg aaaaaaagaa aaatgaactt ggcttatccc aggaatctgt 2221 cgcagacaag atggggatgg ggcagtcagg cgttggtgct ttatttaatg gcatcaatgc 2281 attaaatgct tataacgccg cattgcttgc aaaaattctc aaagttagcg ttgaagaatt 2341 tagcccttca atcgccagag aaatctacga gatgtatgaa gcggttagta tgcagccgtc 2401 acttagaagt gagtatgagt accctgtttt ttctcatgtt caggcaggga tgttctcacc 2461 tgagcttaga acctttacca aaggtgatgc ggagagatgg gtaagcacaa ccaaaaaagc 2521 cagtgattct gcattctggc ttgaggttga aggtaattcc atgaccgcac caacaggctc 2581 caagccaagc tttcctgacg gaatgttaat tctcgttgac cctgagcagg ctgttgagcc 2641 aggtgatttc tgcatagcca gacttggggg tgatgagttt accttcaaga aactgatcag 2701 ggatagcggt caggtgtttt tacaaccact aaacccacag tacccaatga tcccatgcaa 2761 tgagagttgt tccgttgtgg ggaaagttat cgctagtcag tggcctgaag agacgtttgg 2821 cgctgcaaac gacgaaaact acgctttagt agcttaataa cgctgatagt gctagtgtag 2881 atcgctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag 2941 agagagaata taaaaagcca gattattaat ccggcttttt tattattt //