##gff-version 3
##sequence-region BBa_K091182 1 2988
BBa_K091182	.	engineered_region	1318	2988	.	+	0	ID=BBa_K091144;Name=BBa_K091144
BBa_K091182	.	engineered_region	2093	2988	.	+	0	ID=BBa_P0455;Name=BBa_P0455;Parent=BBa_K091144
BBa_K091182	.	terminator	2894	2934	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0455
BBa_K091182	.	polyA_site	2921	2934	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K091182	.	stem_loop	2901	2920	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K091182	.	stop_codon	2927	2927	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K091182	.	engineered_region	2894	2934	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K091182	.	CDS	2111	2860	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_P0455
BBa_K091182	.	tag	2822	2854	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K091182	.	CDS	2114	2821	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K091182	.	engineered_tag	2861	2885	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K091182	.	terminator	2943	2988	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_P0455
BBa_K091182	.	engineered_region	2943	2988	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K091182	.	stem_loop	2955	2977	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K091182	.	ribosome_entry_site	2093	2104	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0455
BBa_K091182	.	conserved_region	2097	2100	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K091182	.	engineered_region	1318	2084	.	+	0	ID=BBa_K091132;Name=BBa_K091132;Parent=BBa_K091144
BBa_K091182	.	engineered_region	1424	2084	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_K091132
BBa_K091182	.	CDS	1442	2059	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K091182	.	CDS	1442	2020	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K091182	.	tag	2021	2052	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K091182	.	engineered_tag	2060	2084	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K091182	.	engineered_region	1442	2059	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K091182	.	ribosome_entry_site	1424	1435	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K091182	.	conserved_region	1428	1431	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K091182	.	promoter	1318	1415	.	+	0	ID=BBa_K091105;Name=BBa_K091105;Parent=BBa_K091132
BBa_K091182	.	operator	1318	1369	.	+	0	ID=annotation1963742;Name=Mnt;Parent=BBa_K091105
BBa_K091182	.	operator	1395	1415	.	+	0	ID=annotation1963741;Name=tetR2;Parent=BBa_K091105
BBa_K091182	.	promoter	1364	1369	.	+	0	ID=annotation1963739;Name=-10;Parent=BBa_K091105
BBa_K091182	.	operator	1370	1388	.	+	0	ID=annotation1963740;Name=tetR1;Parent=BBa_K091105
BBa_K091182	.	engineered_region	1	1309	.	+	0	ID=BBa_K091155;Name=BBa_K091155
BBa_K091182	.	engineered_region	173	1309	.	+	0	ID=BBa_K091154;Name=BBa_K091154;Parent=BBa_K091155
BBa_K091182	.	engineered_region	173	833	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_K091154
BBa_K091182	.	CDS	191	808	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K091182	.	CDS	191	769	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K091182	.	tag	770	801	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K091182	.	engineered_tag	809	833	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K091182	.	engineered_region	191	808	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K091182	.	ribosome_entry_site	173	184	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K091182	.	conserved_region	177	180	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K091182	.	engineered_region	842	1309	.	+	0	ID=BBa_K091141;Name=BBa_K091141;Parent=BBa_K091154
BBa_K091182	.	terminator	1181	1309	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K091141
BBa_K091182	.	terminator	1269	1309	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K091182	.	polyA_site	1296	1309	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K091182	.	stem_loop	1276	1295	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K091182	.	stop_codon	1302	1302	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K091182	.	engineered_region	1269	1309	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K091182	.	terminator	1181	1260	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K091182	.	stem_loop	1192	1235	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K091182	.	engineered_region	1181	1260	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K091182	.	engineered_region	842	1172	.	+	0	ID=BBa_I13025;Name=BBa_I13025;Parent=BBa_K091141
BBa_K091182	.	ribosome_entry_site	842	853	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13025
BBa_K091182	.	conserved_region	846	849	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K091182	.	CDS	860	1147	.	+	0	ID=BBa_C0072;Name=BBa_C0072;Parent=BBa_I13025
BBa_K091182	.	CDS	860	1108	.	+	0	ID=annotation308499;Name=mnt;Parent=BBa_C0072
BBa_K091182	.	tag	1109	1141	.	+	0	ID=annotation308503;Name=LVA;Parent=BBa_C0072
BBa_K091182	.	point_mutation	954	956	.	+	0	ID=annotation308502;Name=T;Parent=BBa_C0072
BBa_K091182	.	engineered_tag	1148	1172	.	+	0	ID=annotation2214010;Name=Help:Barcodes;Parent=BBa_C0072
BBa_K091182	.	promoter	1	164	.	+	0	ID=BBa_K091143;Name=BBa_K091143;Parent=BBa_K091155
BBa_K091182	.	operator	106	126	.	+	0	ID=annotation1967108;Name=plasR OP2;Parent=BBa_K091143
BBa_K091182	.	operator	147	164	.	+	0	ID=annotation1967106;Name=cI OR1;Parent=BBa_K091143
BBa_K091182	.	promoter	117	122	.	+	0	ID=annotation1967105;Name=-35;Parent=BBa_K091143
BBa_K091182	.	promoter	140	145	.	+	0	ID=annotation1967104;Name=-10;Parent=BBa_K091143
BBa_K091182	.	operator	46	63	.	+	0	ID=annotation1967107;Name=plasR OP1;Parent=BBa_K091143
>BBa_K091182
gcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaaca
tacagatttccggcgaaatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacctctggcgg
tgataatactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcgga
ggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataac
cttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgct
ggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaa
agatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaatt
acaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacag
caatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatac
taaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactac
gctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatggcccggg
atgatcctcacttcaattttcgtatgccaatggaagtaagagagaaattgaaatttagagcagaggcaaacggacggag
catgaactctgagcttttgcaaatcgtacaagatgccctaagcaaaccgtcaccagtcactgggtaccgcaatgatgcg
gaacgactcgccgatgagcagagcgagttagtgaagaagatggtcttcgatacactgaaggatctttataaaaaaacca
ccgctgcaaacgacgaaaactacgctttagtagcttaataaccctgatagtgctagtgtagatccctactagagccagg
catcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctact
agagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagctcgaggtgagtgcacagtactaggt
ccacggtgacctagatctcctatagttccctatcagtgatagagaactgtaatccctatcagtgatagagattactaga
gaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggt
attctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatg
agtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacc
tacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatata
gtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactat
ttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatt
tttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgta
ttgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagctt
aataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatgagcacaaaaaagaaaccatt
aacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccag
gaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgctt
ataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacga
gatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcaggg
atgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgatt
ctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgtt
aattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttc
aagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgaga
gttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgc
tttagtagcttaataacgctgatagtgctagtgtagatcgctactagagtcacactggctcaccttcgggtgggccttt
ctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt