BBa_K091182
type: ComponentDefinition
persistent identity: BBa K091182
display id: BBa_K091182
version: 1
was generated by: iGEM to SBOL conversion
description: pLasR/cI-RBS-LuxI-RBS-Mnt-TT-pMnt/tetR-RBS-LuxI-RBS-cI-TT
created: 2008-08-04T11:00:00Z
modified: 2015-05-08T01:08:38Z
mutable provenance: These two parts contain parts that are in the registry and DNA built from oligos.
owned by: james
owned by: myers
mutable description: This part is a combination of the two parts K091155 and K091144.
discontinued: false
dominant: false
experience: experience/Issues
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: no spceial considerations
star: true
creator: Robert Cool
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_K091144
component: BBa_K091155
sequence annotation: BBa_K091155
sequence annotation: BBa_K091144
sequence: https://synbiohub.org/public/igem/BBa_K091182_sequence/1

BBa_K091144
type: Component
persistent identity: component2301935
display id: component2301935
version: 1
top level: BBa_K091182
definition: BBa_K091144
access: public

BBa_K091155
type: Component
persistent identity: component2301908
display id: component2301908
version: 1
top level: BBa_K091182
definition: BBa_K091155
access: public

BBa_K091155
type: SequenceAnnotation
persistent identity: annotation2301908
display id: annotation2301908
version: 1
top level: BBa_K091182
location: https://synbiohub.org/public/igem/BBa_K091182/annotation2301908/range2301908/1
component: BBa_K091155

https://synbiohub.org/public/igem/BBa_K091182/annotation2301908/range2301908/1
type: Range
persistent identity: range2301908
display id: range2301908
version: 1
top level: BBa_K091182
direction: direction/reverse
start: 1
end: 1309
orientation: inline

BBa_K091144
type: SequenceAnnotation
persistent identity: annotation2301935
display id: annotation2301935
version: 1
top level: BBa_K091182
location: https://synbiohub.org/public/igem/BBa_K091182/annotation2301935/range2301935/1
component: BBa_K091144

https://synbiohub.org/public/igem/BBa_K091182/annotation2301935/range2301935/1
type: Range
persistent identity: range2301935
display id: range2301935
version: 1
top level: BBa_K091182
direction: direction/reverse
start: 1318
end: 2988
orientation: inline

BBa_K091144
type: ComponentDefinition
persistent identity: BBa K091144
display id: BBa_K091144
version: 1
was generated by: iGEM to SBOL conversion
description: pMnt/Tet+RBS+LuxI+RBS+cI+TT
created: 2008-07-15T11:00:00Z
modified: 2015-05-08T01:08:38Z
mutable provenance: These parts are in the registry.
owned by: james
owned by: myers
mutable description: This part was made by ligating K091132 to P0455.
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: no special considerations
star: false
creator: Robert Cool
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_P0455
component: BBa_K091132
sequence annotation: BBa_K091132
sequence annotation: BBa_P0455
sequence: https://synbiohub.org/public/igem/BBa_K091144_sequence/1

BBa_P0455
type: Component
persistent identity: component2266731
display id: component2266731
version: 1
top level: BBa_K091144
definition: BBa_P0455
access: public

BBa_K091132
type: Component
persistent identity: component2266718
display id: component2266718
version: 1
top level: BBa_K091144
definition: BBa_K091132
access: public

BBa_K091132
type: SequenceAnnotation
persistent identity: annotation2266718
display id: annotation2266718
version: 1
top level: BBa_K091144
location: https://synbiohub.org/public/igem/BBa_K091144/annotation2266718/range2266718/1
component: BBa_K091132

https://synbiohub.org/public/igem/BBa_K091144/annotation2266718/range2266718/1
type: Range
persistent identity: range2266718
display id: range2266718
version: 1
top level: BBa_K091144
direction: direction/reverse
start: 1
end: 767
orientation: inline

BBa_P0455
type: SequenceAnnotation
persistent identity: annotation2266731
display id: annotation2266731
version: 1
top level: BBa_K091144
location: https://synbiohub.org/public/igem/BBa_K091144/annotation2266731/range2266731/1
component: BBa_P0455

https://synbiohub.org/public/igem/BBa_K091144/annotation2266731/range2266731/1
type: Range
persistent identity: range2266731
display id: range2266731
version: 1
top level: BBa_K091144
direction: direction/reverse
start: 776
end: 1671
orientation: inline

BBa_K091155
type: ComponentDefinition
persistent identity: BBa K091155
display id: BBa_K091155
version: 1
was generated by: iGEM to SBOL conversion
description: plasR/cI+RBS+luxI+RBS+Mnt+TT
created: 2008-07-21T11:00:00Z
modified: 2015-05-08T01:08:38Z
mutable provenance: .
owned by: james
owned by: myers
mutable description: the SW quadrant of our XOR gate.
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3130
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: .
star: false
creator: Xiao Zhu
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_K091143
component: BBa_K091154
sequence annotation: BBa_K091143
sequence annotation: BBa_K091154
sequence: https://synbiohub.org/public/igem/BBa_K091155_sequence/1

BBa_K091143
type: Component
persistent identity: component2266737
display id: component2266737
version: 1
top level: BBa_K091155
definition: BBa_K091143
access: public

BBa_K091154
type: Component
persistent identity: component2266761
display id: component2266761
version: 1
top level: BBa_K091155
definition: BBa_K091154
access: public

BBa_K091143
type: SequenceAnnotation
persistent identity: annotation2266737
display id: annotation2266737
version: 1
top level: BBa_K091155
location: https://synbiohub.org/public/igem/BBa_K091155/annotation2266737/range2266737/1
component: BBa_K091143

https://synbiohub.org/public/igem/BBa_K091155/annotation2266737/range2266737/1
type: Range
persistent identity: range2266737
display id: range2266737
version: 1
top level: BBa_K091155
direction: direction/reverse
start: 1
end: 164
orientation: inline

BBa_K091154
type: SequenceAnnotation
persistent identity: annotation2266761
display id: annotation2266761
version: 1
top level: BBa_K091155
location: https://synbiohub.org/public/igem/BBa_K091155/annotation2266761/range2266761/1
component: BBa_K091154

https://synbiohub.org/public/igem/BBa_K091155/annotation2266761/range2266761/1
type: Range
persistent identity: range2266761
display id: range2266761
version: 1
top level: BBa_K091155
direction: direction/reverse
start: 173
end: 1309
orientation: inline

BBa_K091132
type: ComponentDefinition
persistent identity: BBa K091132
display id: BBa_K091132
version: 1
was generated by: iGEM to SBOL conversion
description: pMnt/Tet+RBS+LuxI
created: 2008-07-10T11:00:00Z
modified: 2015-05-08T01:08:38Z
mutable provenance: These parts are both found in the registry.
owned by: james
owned by: myers
mutable description: This part was made by ligating K091105 (pMnt/Tet) to C0261 (RBS+LuxI).
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: no special considerations
star: false
creator: Robert Cool
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0261
component: BBa_K091105
sequence annotation: BBa_K091105
sequence annotation: BBa_C0261
sequence: https://synbiohub.org/public/igem/BBa_K091132_sequence/1

BBa_C0261
type: Component
persistent identity: component2245884
display id: component2245884
version: 1
top level: BBa_K091132
definition: BBa_C0261
access: public

BBa_K091105
type: Component
persistent identity: component2245877
display id: component2245877
version: 1
top level: BBa_K091132
definition: BBa_K091105
access: public

BBa_K091105
type: SequenceAnnotation
persistent identity: annotation2245877
display id: annotation2245877
version: 1
top level: BBa_K091132
location: https://synbiohub.org/public/igem/BBa_K091132/annotation2245877/range2245877/1
component: BBa_K091105

https://synbiohub.org/public/igem/BBa_K091132/annotation2245877/range2245877/1
type: Range
persistent identity: range2245877
display id: range2245877
version: 1
top level: BBa_K091132
direction: direction/reverse
start: 1
end: 98
orientation: inline

BBa_C0261
type: SequenceAnnotation
persistent identity: annotation2245884
display id: annotation2245884
version: 1
top level: BBa_K091132
location: https://synbiohub.org/public/igem/BBa_K091132/annotation2245884/range2245884/1
component: BBa_C0261

https://synbiohub.org/public/igem/BBa_K091132/annotation2245884/range2245884/1
type: Range
persistent identity: range2245884
display id: range2245884
version: 1
top level: BBa_K091132
direction: direction/reverse
start: 107
end: 767
orientation: inline

BBa_K091143
type: ComponentDefinition
persistent identity: BBa K091143
display id: BBa_K091143
version: 1
was generated by: iGEM to SBOL conversion
description: pLas/cI Hybrid Promoter
created: 2008-07-14T11:00:00Z
modified: 2015-05-08T01:08:38Z
mutable provenance: .
owned by: james
owned by: myers
mutable description: this hybrid promoter is designed to be consecutively off.It combines with plasR(R0079) and lambda cI OR1(see C0051).Adding P-AI-1 sould activate the promoter, while repressed by lambda cI.
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3130
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: "CI binding of the OR region. It binds OR1 with highest affinity, OR2 with next highest, and OR3 with lowest"(http://www.nonanone.com/qw/files/Biology%204450/test2_lecture8,9.txt)
star: true
creator: Xiao Zhu
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: plasR OP1
sequence annotation: plasR OP2
sequence annotation: -35
sequence annotation: -10
sequence annotation: cI OR1
sequence: https://synbiohub.org/public/igem/BBa_K091143_sequence/1

plasR OP1
type: SequenceAnnotation
persistent identity: annotation1967107
display id: annotation1967107
version: 1
top level: BBa_K091143
location: https://synbiohub.org/public/igem/BBa_K091143/annotation1967107/range1967107/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K091143/annotation1967107/range1967107/1
type: Range
persistent identity: range1967107
display id: range1967107
version: 1
top level: BBa_K091143
direction: direction/forward
start: 46
end: 63
orientation: inline

plasR OP2
type: SequenceAnnotation
persistent identity: annotation1967108
display id: annotation1967108
version: 1
top level: BBa_K091143
location: https://synbiohub.org/public/igem/BBa_K091143/annotation1967108/range1967108/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_K091143/annotation1967108/range1967108/1
type: Range
persistent identity: range1967108
display id: range1967108
version: 1
top level: BBa_K091143
direction: direction/forward
start: 106
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1967105
display id: annotation1967105
version: 1
top level: BBa_K091143
location: https://synbiohub.org/public/igem/BBa_K091143/annotation1967105/range1967105/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K091143/annotation1967105/range1967105/1
type: Range
persistent identity: range1967105
display id: range1967105
version: 1
top level: BBa_K091143
direction: direction/forward
start: 117
end: 122
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1967104
display id: annotation1967104
version: 1
top level: BBa_K091143
location: https://synbiohub.org/public/igem/BBa_K091143/annotation1967104/range1967104/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K091143/annotation1967104/range1967104/1
type: Range
persistent identity: range1967104
display id: range1967104
version: 1
top level: BBa_K091143
direction: direction/forward
start: 140
end: 145
orientation: inline

cI OR1
type: SequenceAnnotation
persistent identity: annotation1967106
display id: annotation1967106
version: 1
top level: BBa_K091143
location: https://synbiohub.org/public/igem/BBa_K091143/annotation1967106/range1967106/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K091143/annotation1967106/range1967106/1
type: Range
persistent identity: range1967106
display id: range1967106
version: 1
top level: BBa_K091143
direction: direction/forward
start: 147
end: 164
orientation: inline

BBa_P0455
type: ComponentDefinition
persistent identity: BBa P0455
display id: BBa_P0455
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.Lambda cI LVA+.Term
created: 2005-06-29T11:00:00Z
modified: 2015-05-08T01:14:11Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 6
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_C0051
component: BBa_B0034
component: BBa_B0011
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_C0051
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_P0455_sequence/1

BBa_C0051
type: Component
persistent identity: component1558073
display id: component1558073
version: 1
top level: BBa_P0455
definition: cI lam
access: public

BBa_B0034
type: Component
persistent identity: component1558063
display id: component1558063
version: 1
top level: BBa_P0455
definition: BBa_B0034
access: public

BBa_B0011
type: Component
persistent identity: component1558091
display id: component1558091
version: 1
top level: BBa_P0455
definition: BBa_B0011
access: public

BBa_B0012
type: Component
persistent identity: component1558083
display id: component1558083
version: 1
top level: BBa_P0455
definition: BBa_B0012
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1558063
display id: annotation1558063
version: 1
top level: BBa_P0455
location: https://synbiohub.org/public/igem/BBa_P0455/annotation1558063/range1558063/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_P0455/annotation1558063/range1558063/1
type: Range
persistent identity: range1558063
display id: range1558063
version: 1
top level: BBa_P0455
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation1558073
display id: annotation1558073
version: 1
top level: BBa_P0455
location: https://synbiohub.org/public/igem/BBa_P0455/annotation1558073/range1558073/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_P0455/annotation1558073/range1558073/1
type: Range
persistent identity: range1558073
display id: range1558073
version: 1
top level: BBa_P0455
direction: direction/forward
start: 19
end: 768
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1558083
display id: annotation1558083
version: 1
top level: BBa_P0455
location: https://synbiohub.org/public/igem/BBa_P0455/annotation1558083/range1558083/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0455/annotation1558083/range1558083/1
type: Range
persistent identity: range1558083
display id: range1558083
version: 1
top level: BBa_P0455
direction: direction/forward
start: 802
end: 842
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation1558091
display id: annotation1558091
version: 1
top level: BBa_P0455
location: https://synbiohub.org/public/igem/BBa_P0455/annotation1558091/range1558091/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_P0455/annotation1558091/range1558091/1
type: Range
persistent identity: range1558091
display id: range1558091
version: 1
top level: BBa_P0455
direction: direction/forward
start: 851
end: 896
orientation: inline

BBa_K091154
type: ComponentDefinition
persistent identity: BBa K091154
display id: BBa_K091154
version: 1
was generated by: iGEM to SBOL conversion
description: RBS+luxI+RBS+Mnt+TT
created: 2008-07-21T11:00:00Z
modified: 2015-05-08T01:08:38Z
mutable provenance: .
owned by: james
owned by: myers
mutable description: RBS here is B0034, TT here is B0015, luxI has LVA tag
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3130
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: .
star: false
creator: Xiao Zhu
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0261
component: BBa_K091141
sequence annotation: BBa_C0261
sequence annotation: BBa_K091141
sequence: https://synbiohub.org/public/igem/BBa_K091154_sequence/1

BBa_C0261
type: Component
persistent identity: component2261562
display id: component2261562
version: 1
top level: BBa_K091154
definition: BBa_C0261
access: public

BBa_K091141
type: Component
persistent identity: component2261578
display id: component2261578
version: 1
top level: BBa_K091154
definition: BBa_K091141
access: public

BBa_C0261
type: SequenceAnnotation
persistent identity: annotation2261562
display id: annotation2261562
version: 1
top level: BBa_K091154
location: https://synbiohub.org/public/igem/BBa_K091154/annotation2261562/range2261562/1
component: BBa_C0261

https://synbiohub.org/public/igem/BBa_K091154/annotation2261562/range2261562/1
type: Range
persistent identity: range2261562
display id: range2261562
version: 1
top level: BBa_K091154
direction: direction/reverse
start: 1
end: 661
orientation: inline

BBa_K091141
type: SequenceAnnotation
persistent identity: annotation2261578
display id: annotation2261578
version: 1
top level: BBa_K091154
location: https://synbiohub.org/public/igem/BBa_K091154/annotation2261578/range2261578/1
component: BBa_K091141

https://synbiohub.org/public/igem/BBa_K091154/annotation2261578/range2261578/1
type: Range
persistent identity: range2261578
display id: range2261578
version: 1
top level: BBa_K091154
direction: direction/reverse
start: 670
end: 1137
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_C0261
type: ComponentDefinition
persistent identity: BBa C0261
display id: BBa_C0261
version: 1
was generated by: iGEM to SBOL conversion
description: AHL-making Enzyme, luxI (+RBS)
created: 2004-08-22T11:00:00Z
modified: 2015-08-31T04:07:24Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: B0034.C0061
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
sample status: In stock
status: status/Available
bookmark: true
star: true
creator: robertb
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_C0061
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0061
sequence: https://synbiohub.org/public/igem/BBa_C0261_sequence/1

BBa_C0061
type: Component
persistent identity: component1060413
display id: component1060413
version: 1
top level: BBa_C0261
definition: luxI
access: public

BBa_B0034
type: Component
persistent identity: component1060403
display id: component1060403
version: 1
top level: BBa_C0261
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1060403
display id: annotation1060403
version: 1
top level: BBa_C0261
location: https://synbiohub.org/public/igem/BBa_C0261/annotation1060403/range1060403/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_C0261/annotation1060403/range1060403/1
type: Range
persistent identity: range1060403
display id: range1060403
version: 1
top level: BBa_C0261
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation1060413
display id: annotation1060413
version: 1
top level: BBa_C0261
location: https://synbiohub.org/public/igem/BBa_C0261/annotation1060413/range1060413/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_C0261/annotation1060413/range1060413/1
type: Range
persistent identity: range1060413
display id: range1060413
version: 1
top level: BBa_C0261
direction: direction/forward
start: 19
end: 636
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa C0051
display id: BBa_C0051
version: 1
was generated by: iGEM to SBOL conversion
description: cI repressor from E. coli phage lambda (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051).
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 BBa_C0051 cI repressor is based on the cI repressor from the Elowitz's repressilator. It has been modified to include a rapid degradation LAA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cI lambda
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0051_sequence/1

cI lambda
type: SequenceAnnotation
persistent identity: annotation23334
display id: annotation23334
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
type: Range
persistent identity: range23334
display id: range23334
version: 1
top level: cI lam
direction: direction/forward
start: 4
end: 711
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation23335
display id: annotation23335
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
type: Range
persistent identity: range23335
display id: range23335
version: 1
top level: cI lam
direction: direction/forward
start: 712
end: 744
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213991
display id: annotation2213991
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
type: Range
persistent identity: range2213991
display id: range2213991
version: 1
top level: cI lam
direction: direction/forward
start: 751
end: 775
orientation: inline

BBa_K091105
type: ComponentDefinition
persistent identity: BBa K091105
display id: BBa_K091105
version: 1
was generated by: iGEM to SBOL conversion
description: pTet/Mnt Hybrid Promoter
created: 2008-06-18T11:00:00Z
modified: 2015-05-08T01:08:37Z
mutable provenance: This part comes from the Mnt promoter and the TetR promoter
owned by: james
owned by: myers
mutable description: This promoter is a modified version of the Mnt promoter that is also responsive to TetR. The promoter should be repressed by Mnt repressor. It should also be repressed by TetR, and in the absence of Mnt repressor, should be induced by aTc.
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Mnt repressor binds as a tetramer to two half-operator sites. Introduction of TetR binding sites between the Mnt promoter -10 sequence and the start site for transcription should allow for repression by TetR. Accordingly, the hybrid promoter was designed by 1) Remove all bases of mnt promoter 3??? to -10: gagtcgtattaattt will be replaced by tccctatcagtgata, and 2) truncate the second half of ptet -10 and mutate -35 so that it function to bind tetR (replace ttgaca with actgta), but is not a promoter, and 3) place the tetR1 and tetR2 binding sites 3??? to truncated mnt -10 sequence.
star: true
creator: Robert Cool
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Mnt
sequence annotation: -10
sequence annotation: tetR1
sequence annotation: tetR2
sequence: https://synbiohub.org/public/igem/BBa_K091105_sequence/1

Mnt
type: SequenceAnnotation
persistent identity: annotation1963742
display id: annotation1963742
version: 1
top level: BBa_K091105
location: https://synbiohub.org/public/igem/BBa_K091105/annotation1963742/range1963742/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K091105/annotation1963742/range1963742/1
type: Range
persistent identity: range1963742
display id: range1963742
version: 1
top level: BBa_K091105
direction: direction/forward
start: 1
end: 52
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1963739
display id: annotation1963739
version: 1
top level: BBa_K091105
location: https://synbiohub.org/public/igem/BBa_K091105/annotation1963739/range1963739/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K091105/annotation1963739/range1963739/1
type: Range
persistent identity: range1963739
display id: range1963739
version: 1
top level: BBa_K091105
direction: direction/forward
start: 47
end: 52
orientation: inline

tetR1
type: SequenceAnnotation
persistent identity: annotation1963740
display id: annotation1963740
version: 1
top level: BBa_K091105
location: https://synbiohub.org/public/igem/BBa_K091105/annotation1963740/range1963740/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K091105/annotation1963740/range1963740/1
type: Range
persistent identity: range1963740
display id: range1963740
version: 1
top level: BBa_K091105
direction: direction/forward
start: 53
end: 71
orientation: inline

tetR2
type: SequenceAnnotation
persistent identity: annotation1963741
display id: annotation1963741
version: 1
top level: BBa_K091105
location: https://synbiohub.org/public/igem/BBa_K091105/annotation1963741/range1963741/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K091105/annotation1963741/range1963741/1
type: Range
persistent identity: range1963741
display id: range1963741
version: 1
top level: BBa_K091105
direction: direction/forward
start: 78
end: 98
orientation: inline

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: iGEM to SBOL conversion
description: LuxICDABEG (+/-)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0011
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
type: Range
persistent identity: range7019
display id: range7019
version: 1
top level: BBa_B0011
direction: direction/forward
start: 1
end: 46
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
type: Range
persistent identity: range1683
display id: range1683
version: 1
top level: BBa_B0011
direction: direction/forward
start: 13
end: 35
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

luxI
type: ComponentDefinition
persistent identity: BBa C0061
display id: BBa_C0061
version: 1
was generated by: iGEM to SBOL conversion
description: autoinducer synthetase for AHL
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Synthesizes 3OC6HSL, which binds to LuxR. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound HSL. This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: An LVA tail (sequence: AANDENYALVA) was added to increase protein degradation. .
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_C0061
sequence annotation: luxI
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0061_sequence/1

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation7038
display id: annotation7038
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1
type: Range
persistent identity: range7038
display id: range7038
version: 1
top level: luxI
direction: direction/forward
start: 1
end: 618
orientation: inline

luxI
type: SequenceAnnotation
persistent identity: annotation1761
display id: annotation1761
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1
type: Range
persistent identity: range1761
display id: range1761
version: 1
top level: luxI
direction: direction/forward
start: 1
end: 579
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1760
display id: annotation1760
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1
type: Range
persistent identity: range1760
display id: range1760
version: 1
top level: luxI
direction: direction/forward
start: 580
end: 611
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213985
display id: annotation2213985
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1
type: Range
persistent identity: range2213985
display id: range2213985
version: 1
top level: luxI
direction: direction/forward
start: 619
end: 643
orientation: inline

BBa_K091141
type: ComponentDefinition
persistent identity: BBa K091141
display id: BBa_K091141
version: 1
was generated by: iGEM to SBOL conversion
description: RBS+Mnt+TT
created: 2008-07-14T11:00:00Z
modified: 2015-05-08T01:08:38Z
mutable provenance: .
owned by: james
owned by: myers
mutable description: I13025 with double terminator B0015
discontinued: false
dominant: false
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 3130
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: no.
star: false
creator: Xiao Zhu
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0015
component: BBa_I13025
sequence annotation: BBa_I13025
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K091141_sequence/1

BBa_B0015
type: Component
persistent identity: component2249133
display id: component2249133
version: 1
top level: BBa_K091141
definition: BBa_B0015
access: public

BBa_I13025
type: Component
persistent identity: component2249126
display id: component2249126
version: 1
top level: BBa_K091141
definition: BBa_I13025
access: public

BBa_I13025
type: SequenceAnnotation
persistent identity: annotation2249126
display id: annotation2249126
version: 1
top level: BBa_K091141
location: https://synbiohub.org/public/igem/BBa_K091141/annotation2249126/range2249126/1
component: BBa_I13025

https://synbiohub.org/public/igem/BBa_K091141/annotation2249126/range2249126/1
type: Range
persistent identity: range2249126
display id: range2249126
version: 1
top level: BBa_K091141
direction: direction/reverse
start: 1
end: 331
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2249133
display id: annotation2249133
version: 1
top level: BBa_K091141
location: https://synbiohub.org/public/igem/BBa_K091141/annotation2249133/range2249133/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K091141/annotation2249133/range2249133/1
type: Range
persistent identity: range2249133
display id: range2249133
version: 1
top level: BBa_K091141
direction: direction/reverse
start: 340
end: 468
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_I13025
type: ComponentDefinition
persistent identity: BBa I13025
display id: BBa_I13025
version: 1
was generated by: iGEM to SBOL conversion
description: mnt QPI Intermediate
created: 2004-07-14T11:00:00Z
modified: 2015-08-31T04:07:32Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: jenmitch
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_C0072
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0072
sequence: https://synbiohub.org/public/igem/BBa_I13025_sequence/1

BBa_C0072
type: Component
persistent identity: component939424
display id: component939424
version: 1
top level: BBa_I13025
definition: mnt (s)
access: public

BBa_B0034
type: Component
persistent identity: component939411
display id: component939411
version: 1
top level: BBa_I13025
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation939411
display id: annotation939411
version: 1
top level: BBa_I13025
location: https://synbiohub.org/public/igem/BBa_I13025/annotation939411/range939411/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13025/annotation939411/range939411/1
type: Range
persistent identity: range939411
display id: range939411
version: 1
top level: BBa_I13025
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0072
type: SequenceAnnotation
persistent identity: annotation939424
display id: annotation939424
version: 1
top level: BBa_I13025
location: https://synbiohub.org/public/igem/BBa_I13025/annotation939424/range939424/1
component: BBa_C0072

https://synbiohub.org/public/igem/BBa_I13025/annotation939424/range939424/1
type: Range
persistent identity: range939424
display id: range939424
version: 1
top level: BBa_I13025
direction: direction/forward
start: 19
end: 306
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

mnt (s)
type: ComponentDefinition
persistent identity: BBa C0072
display id: BBa_C0072
version: 1
was generated by: iGEM to SBOL conversion
description: mnt repressor (strong) from Salmonella phage P22 (+LVA)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: enterobacteriophage p22
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: wild type dimeric repressor from enterobacteriophage p22 with dissociation constant of 2.5*10^-12. sequence was translated with the E.Coli K12 codon usage table, and nucleotides 95-97 was changed from AAT to AAC to avoid the cut site GAATTC (94-99). Identification of Functionally Important Residues in the DNA Binding Region of the MNT Repressor Kendall, KL; Sauer, RT The Journal of Biological Chemistry, 264(23):13706-13710, 1989
star: true
creator: crackdots
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mnt
sequence annotation: T
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0072_sequence/1

mnt
type: SequenceAnnotation
persistent identity: annotation308499
display id: annotation308499
version: 1
top level: mnt (s)
location: https://synbiohub.org/public/igem/BBa_C0072/annotation308499/range308499/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0072/annotation308499/range308499/1
type: Range
persistent identity: range308499
display id: range308499
version: 1
top level: mnt (s)
direction: direction/forward
start: 1
end: 249
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation308502
display id: annotation308502
version: 1
top level: mnt (s)
location: https://synbiohub.org/public/igem/BBa_C0072/annotation308502/range308502/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0072/annotation308502/range308502/1
type: Range
persistent identity: range308502
display id: range308502
version: 1
top level: mnt (s)
direction: direction/forward
start: 95
end: 97
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation308503
display id: annotation308503
version: 1
top level: mnt (s)
location: https://synbiohub.org/public/igem/BBa_C0072/annotation308503/range308503/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0072/annotation308503/range308503/1
type: Range
persistent identity: range308503
display id: range308503
version: 1
top level: mnt (s)
direction: direction/forward
start: 250
end: 282
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214010
display id: annotation2214010
version: 1
top level: mnt (s)
location: https://synbiohub.org/public/igem/BBa_C0072/annotation2214010/range2214010/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0072/annotation2214010/range2214010/1
type: Range
persistent identity: range2214010
display id: range2214010
version: 1
top level: mnt (s)
direction: direction/forward
start: 289
end: 313
orientation: inline

https://synbiohub.org/public/igem/BBa_K091182_sequence/1
type: Sequence
persistent identity: BBa K091182 sequence
display id: BBa_K091182_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacctctggcggtgataatactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatggcccgggatgatcctcacttcaattttcgtatgccaatggaagtaagagagaaattgaaatttagagcagaggcaaacggacggagcatgaactctgagcttttgcaaatcgtacaagatgccctaagcaaaccgtcaccagtcactgggtaccgcaatgatgcggaacgactcgccgatgagcagagcgagttagtgaagaagatggtcttcgatacactgaaggatctttataaaaaaaccaccgctgcaaacgacgaaaactacgctttagtagcttaataaccctgatagtgctagtgtagatccctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagctcgaggtgagtgcacagtactaggtccacggtgacctagatctcctatagttccctatcagtgatagagaactgtaatccctatcagtgatagagattactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K091144_sequence/1
type: Sequence
persistent identity: BBa K091144 sequence
display id: BBa_K091144_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcgaggtgagtgcacagtactaggtccacggtgacctagatctcctatagttccctatcagtgatagagaactgtaatccctatcagtgatagagattactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K091155_sequence/1
type: Sequence
persistent identity: BBa K091155 sequence
display id: BBa_K091155_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacctctggcggtgataatactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatggcccgggatgatcctcacttcaattttcgtatgccaatggaagtaagagagaaattgaaatttagagcagaggcaaacggacggagcatgaactctgagcttttgcaaatcgtacaagatgccctaagcaaaccgtcaccagtcactgggtaccgcaatgatgcggaacgactcgccgatgagcagagcgagttagtgaagaagatggtcttcgatacactgaaggatctttataaaaaaaccaccgctgcaaacgacgaaaactacgctttagtagcttaataaccctgatagtgctagtgtagatccctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K091132_sequence/1
type: Sequence
persistent identity: BBa K091132 sequence
display id: BBa_K091132_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcgaggtgagtgcacagtactaggtccacggtgacctagatctcctatagttccctatcagtgatagagaactgtaatccctatcagtgatagagattactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K091143_sequence/1
type: Sequence
persistent identity: BBa K091143 sequence
display id: BBa_K091143_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacctctggcggtgataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_P0455_sequence/1
type: Sequence
persistent identity: BBa P0455 sequence
display id: BBa_P0455_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K091154_sequence/1
type: Sequence
persistent identity: BBa K091154 sequence
display id: BBa_K091154_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatggcccgggatgatcctcacttcaattttcgtatgccaatggaagtaagagagaaattgaaatttagagcagaggcaaacggacggagcatgaactctgagcttttgcaaatcgtacaagatgccctaagcaaaccgtcaccagtcactgggtaccgcaatgatgcggaacgactcgccgatgagcagagcgagttagtgaagaagatggtcttcgatacactgaaggatctttataaaaaaaccaccgctgcaaacgacgaaaactacgctttagtagcttaataaccctgatagtgctagtgtagatccctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0261_sequence/1
type: Sequence
persistent identity: BBa C0261 sequence
display id: BBa_C0261_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0051_sequence/1
type: Sequence
persistent identity: BBa C0051 sequence
display id: BBa_C0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K091105_sequence/1
type: Sequence
persistent identity: BBa K091105 sequence
display id: BBa_K091105_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcgaggtgagtgcacagtactaggtccacggtgacctagatctcctatagttccctatcagtgatagagaactgtaatccctatcagtgatagagat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0011_sequence/1
type: Sequence
persistent identity: BBa B0011 sequence
display id: BBa_B0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0061_sequence/1
type: Sequence
persistent identity: BBa C0061 sequence
display id: BBa_C0061_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K091141_sequence/1
type: Sequence
persistent identity: BBa K091141 sequence
display id: BBa_K091141_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggcccgggatgatcctcacttcaattttcgtatgccaatggaagtaagagagaaattgaaatttagagcagaggcaaacggacggagcatgaactctgagcttttgcaaatcgtacaagatgccctaagcaaaccgtcaccagtcactgggtaccgcaatgatgcggaacgactcgccgatgagcagagcgagttagtgaagaagatggtcttcgatacactgaaggatctttataaaaaaaccaccgctgcaaacgacgaaaactacgctttagtagcttaataaccctgatagtgctagtgtagatccctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13025_sequence/1
type: Sequence
persistent identity: BBa I13025 sequence
display id: BBa_I13025_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggcccgggatgatcctcacttcaattttcgtatgccaatggaagtaagagagaaattgaaatttagagcagaggcaaacggacggagcatgaactctgagcttttgcaaatcgtacaagatgccctaagcaaaccgtcaccagtcactgggtaccgcaatgatgcggaacgactcgccgatgagcagagcgagttagtgaagaagatggtcttcgatacactgaaggatctttataaaaaaaccaccgctgcaaacgacgaaaactacgctttagtagcttaataaccctgatagtgctagtgtagatccc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0072_sequence/1
type: Sequence
persistent identity: BBa C0072 sequence
display id: BBa_C0072_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcccgggatgatcctcacttcaattttcgtatgccaatggaagtaagagagaaattgaaatttagagcagaggcaaacggacggagcatgaactctgagcttttgcaaatcgtacaagatgccctaagcaaaccgtcaccagtcactgggtaccgcaatgatgcggaacgactcgccgatgagcagagcgagttagtgaagaagatggtcttcgatacactgaaggatctttataaaaaaaccaccgctgcaaacgacgaaaactacgctttagtagcttaataaccctgatagtgctagtgtagatccc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z