LOCUS BBa_K091184 2616 bp DNA linear 22-Dec-2024 DEFINITION pLux/cI+RBS+LuxS+RBS+Mnt+TT+pLac/Mnt+RBS+LuxS+RBS+cI+TT ACCESSION BBa_K091184 VERSION BBa_K091184.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=Lux Box promoter 20..25 /label=Lux -35 misc_feature 26..43 /label=cI OR2 promoter 44..49 /label=Lux -10 promoter 1..57 /label=BBa_K091107 misc_feature 70..73 /label=conserved RBS 66..77 /label=BBa_B0034 CDS 84..599 /label=BBa_K091109 misc_feature 66..599 /label=BBa_K091153 misc_feature 612..615 /label=conserved RBS 608..619 /label=BBa_B0034 CDS 626..874 /label=mnt misc_feature 720..722 /label=T misc_feature 875..907 /label=LVA misc_feature 914..938 /label=Help:Barcodes CDS 626..913 /label=BBa_C0072 misc_feature 608..938 /label=BBa_I13025 stem_loop 958..1001 /label=stem_loop misc_feature 947..1026 /label=BBa_B0010 terminator 947..1026 /label=BBa_B0010 stem_loop 1042..1061 /label=T7 TE misc_feature 1068..1068 /label=stop polyA_site 1062..1075 /label=polya misc_feature 1035..1075 /label=BBa_B0012 terminator 1035..1075 /label=BBa_B0012 terminator 947..1075 /label=BBa_B0015 misc_feature 608..1075 /label=BBa_K091137 misc_feature 66..1075 /label=BBa_K091163 misc_feature 1..1075 /label=BBa_K091164 promoter 1130..1135 /label=-10 misc_feature 1136..1170 /label=LacI misc_feature 1084..1135 /label=Mnt promoter 1084..1170 /label=BBa_K091104 misc_feature 1183..1186 /label=conserved RBS 1179..1190 /label=BBa_B0034 CDS 1197..1712 /label=BBa_K091109 misc_feature 1179..1712 /label=BBa_S03968 misc_feature 1725..1728 /label=conserved RBS 1721..1732 /label=BBa_B0034 CDS 1742..2449 /label=cI lambda misc_feature 2450..2482 /label=LVA misc_feature 2489..2513 /label=Help:Barcodes CDS 1739..2488 /label=BBa_C0051 stem_loop 2529..2548 /label=T7 TE misc_feature 2555..2555 /label=stop polyA_site 2549..2562 /label=polya misc_feature 2522..2562 /label=BBa_B0012 terminator 2522..2562 /label=BBa_B0012 stem_loop 2583..2605 /label=stem_loop misc_feature 2571..2616 /label=BBa_B0011 terminator 2571..2616 /label=BBa_B0011 misc_feature 1721..2616 /label=BBa_P0455 misc_feature 1179..2616 /label=BBa_S03979 misc_feature 1084..2616 /label=BBa_K091168 ORIGIN 1 acctgtagga tcgtacaggt ttacgtaaca ccgtgcgtgt tgatatagtc gaataaatac 61 tagagaaaga ggagaaatac tagatgccgt tgttagatag cttcacagtc gatcataccc 121 ggatggaagc gcctgctgtt cgggtggcga aaacaatgaa caccccgcat ggcgacgcaa 181 tcaccgtgtt cgatctgcgc ttctgcgtgc cgaacaaaga agtgatgcca gaaagaggga 241 tccataccct ggagcacctg tttgctggtt ttatgcgtaa ccatcttaac ggtaatggtg 301 tagagattat cgatatctcg ccaatgggct gccgcaccgg tttttatatg agtctgattg 361 gtacgccaga tgagcagcgt gttgctgatg cctggaaagc ggcaatggaa gacgtgctga 421 aagtgcagga tcagaatcag atcccggaac tgaacgtcta ccagtgtggc acttaccaga 481 tgcactcgtt gcaggaagcg caggatattg cgcgtagcat tctggaacgt gacgtacgca 541 tcaacagcaa cgaagaactg gcactgccga aagagaagtt gcaggaactg cacatctaat 601 actagagaaa gaggagaaat actagatggc ccgggatgat cctcacttca attttcgtat 661 gccaatggaa gtaagagaga aattgaaatt tagagcagag gcaaacggac ggagcatgaa 721 ctctgagctt ttgcaaatcg tacaagatgc cctaagcaaa ccgtcaccag tcactgggta 781 ccgcaatgat gcggaacgac tcgccgatga gcagagcgag ttagtgaaga agatggtctt 841 cgatacactg aaggatcttt ataaaaaaac caccgctgca aacgacgaaa actacgcttt 901 agtagcttaa taaccctgat agtgctagtg tagatcccta ctagagccag gcatcaaata 961 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac 1021 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta 1081 gagctcgagg tgagtgcaca gtactaggtc cacggtgacc tagatctcct atagttgtgt 1141 ggaattgtga gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc 1201 cgttgttaga tagcttcaca gtcgatcata cccggatgga agcgcctgct gttcgggtgg 1261 cgaaaacaat gaacaccccg catggcgacg caatcaccgt gttcgatctg cgcttctgcg 1321 tgccgaacaa agaagtgatg ccagaaagag ggatccatac cctggagcac ctgtttgctg 1381 gttttatgcg taaccatctt aacggtaatg gtgtagagat tatcgatatc tcgccaatgg 1441 gctgccgcac cggtttttat atgagtctga ttggtacgcc agatgagcag cgtgttgctg 1501 atgcctggaa agcggcaatg gaagacgtgc tgaaagtgca ggatcagaat cagatcccgg 1561 aactgaacgt ctaccagtgt ggcacttacc agatgcactc gttgcaggaa gcgcaggata 1621 ttgcgcgtag cattctggaa cgtgacgtac gcatcaacag caacgaagaa ctggcactgc 1681 cgaaagagaa gttgcaggaa ctgcacatct aatactagag aaagaggaga aatactagat 1741 gagcacaaaa aagaaaccat taacacaaga gcagcttgag gacgcacgtc gccttaaagc 1801 aatttatgaa aaaaagaaaa atgaacttgg cttatcccag gaatctgtcg cagacaagat 1861 ggggatgggg cagtcaggcg ttggtgcttt atttaatggc atcaatgcat taaatgctta 1921 taacgccgca ttgcttgcaa aaattctcaa agttagcgtt gaagaattta gcccttcaat 1981 cgccagagaa atctacgaga tgtatgaagc ggttagtatg cagccgtcac ttagaagtga 2041 gtatgagtac cctgtttttt ctcatgttca ggcagggatg ttctcacctg agcttagaac 2101 ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc aaaaaagcca gtgattctgc 2161 attctggctt gaggttgaag gtaattccat gaccgcacca acaggctcca agccaagctt 2221 tcctgacgga atgttaattc tcgttgaccc tgagcaggct gttgagccag gtgatttctg 2281 catagccaga cttgggggtg atgagtttac cttcaagaaa ctgatcaggg atagcggtca 2341 ggtgttttta caaccactaa acccacagta cccaatgatc ccatgcaatg agagttgttc 2401 cgttgtgggg aaagttatcg ctagtcagtg gcctgaagag acgtttggcg ctgcaaacga 2461 cgaaaactac gctttagtag cttaataacg ctgatagtgc tagtgtagat cgctactaga 2521 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag agagaatata 2581 aaaagccaga ttattaatcc ggctttttta ttattt //