LOCUS       BBa_K091186             2528 bp    DNA     linear       24-Jun-2024
DEFINITION  pBAD+RBS+LacI+TT+pBAD+RBS+LuxR+TT
ACCESSION   BBa_K091186
VERSION     BBa_K091186.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=BBa_I13453
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     misc_feature    1246..1284
                     /label=LVA
     CDS             157..1284
                     /label=lacI-LVA
     misc_feature    157..1284
                     /label=BBa_C0012
     misc_feature    1285..1309
                     /label=Help:Barcodes
     CDS             157..1284
                     /label=BBa_C0012
     stem_loop       1329..1372
                     /label=stem_loop
     misc_feature    1318..1397
                     /label=BBa_B0010
     terminator      1318..1397
                     /label=BBa_B0010
     stem_loop       1413..1432
                     /label=T7 TE
     misc_feature    1439..1439
                     /label=stop
     polyA_site      1433..1446
                     /label=polya
     misc_feature    1406..1446
                     /label=BBa_B0012
     terminator      1406..1446
                     /label=BBa_B0012
     misc_feature    139..1446
                     /label=BBa_P0412
     misc_feature    1..1446
                     /label=BBa_S03980
     promoter        1455..1584
                     /label=BBa_I13453
     misc_feature    1597..1600
                     /label=conserved
     RBS             1593..1604
                     /label=BBa_B0034
     misc_feature    1611..1612
                     /label=prefix
     misc_feature    1784..1784
                     /label=T
     misc_feature    2102..2102
                     /label=A
     CDS             1611..2360
                     /label=luxR
     misc_feature    1611..2366
                     /label=BBa_C0062
     misc_feature    2367..2391
                     /label=Help:Barcodes
     CDS             1611..2366
                     /label=BBa_C0062
     misc_feature    1593..2391
                     /label=BBa_J37033
     misc_feature    1455..2391
                     /label=BBa_K091166
     stem_loop       2411..2454
                     /label=stem_loop
     misc_feature    2400..2479
                     /label=BBa_B0010
     terminator      2400..2479
                     /label=BBa_B0010
     stem_loop       2495..2514
                     /label=T7 TE
     misc_feature    2521..2521
                     /label=stop
     polyA_site      2515..2528
                     /label=polya
     misc_feature    2488..2528
                     /label=BBa_B0012
     terminator      2488..2528
                     /label=BBa_B0012
     terminator      2400..2528
                     /label=BBa_B0015
     misc_feature    1455..2528
                     /label=BBa_K091179
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatgg tgaatgtgaa accagtaacg
      181 ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac
      241 caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg
      301 aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc
      361 gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct
      421 cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa
      481 gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac
      541 tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg
      601 ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac
      661 ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta
      721 gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc
      781 actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt
      841 tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc
      901 aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt
      961 gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg
     1021 ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg
     1081 caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa
     1141 agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     1201 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcaggctgc aaacgacgaa
     1261 aactacgctt tagtagctta ataactctga tagtgctagt gtagatctct actagagcca
     1321 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     1381 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
     1441 tttatatact agagacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt
     1501 tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc
     1561 tccataccgt ttttttgggc tagctactag agaaagagga gaaatactag atgaaaaaca
     1621 taaatgccga cgacacatac agaataatta ataaaattaa agcttgtaga agcaataatg
     1681 atattaatca atgcttatct gatatgacta aaatggtaca ttgtgaatat tatttactcg
     1741 cgatcattta tcctcattct atggttaaat ctgatatttc aatcctagat aattacccta
     1801 aaaaatggag gcaatattat gatgacgcta atttaataaa atatgatcct atagtagatt
     1861 attctaactc caatcattca ccaattaatt ggaatatatt tgaaaacaat gctgtaaata
     1921 aaaaatctcc aaatgtaatt aaagaagcga aaacatcagg tcttatcact gggtttagtt
     1981 tccctattca tacggctaac aatggcttcg gaatgcttag ttttgcacat tcagaaaaag
     2041 acaactatat agatagttta tttttacatg cgtgtatgaa cataccatta attgttcctt
     2101 ctctagttga taattatcga aaaataaata tagcaaataa taaatcaaac aacgatttaa
     2161 ccaaaagaga aaaagaatgt ttagcgtggg catgcgaagg aaaaagctct tgggatattt
     2221 caaaaatatt aggttgcagt gagcgtactg tcactttcca tttaaccaat gcgcaaatga
     2281 aactcaatac aacaaaccgc tgccaaagta tttctaaagc aattttaaca ggagcaattg
     2341 attgcccata ctttaaaaat taataacact gatagtgcta gtgtagatca ctactagagc
     2401 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     2461 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     2521 cgtttata
//