##gff-version 3
##sequence-region BBa_K091186 1 2528
BBa_K091186	.	engineered_region	1	1446	.	+	0	ID=BBa_S03980;Name=BBa_S03980
BBa_K091186	.	promoter	1	130	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_S03980
BBa_K091186	.	engineered_region	139	1446	.	+	0	ID=BBa_P0412;Name=BBa_P0412;Parent=BBa_S03980
BBa_K091186	.	terminator	1406	1446	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0412
BBa_K091186	.	engineered_region	1406	1446	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K091186	.	stop_codon	1439	1439	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K091186	.	stem_loop	1413	1432	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K091186	.	polyA_site	1433	1446	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K091186	.	CDS	157	1284	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_P0412
BBa_K091186	.	engineered_tag	1285	1309	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K091186	.	engineered_region	157	1284	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K091186	.	CDS	157	1284	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K091186	.	tag	1246	1284	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K091186	.	ribosome_entry_site	139	150	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0412
BBa_K091186	.	conserved_region	143	146	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K091186	.	terminator	1318	1397	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0412
BBa_K091186	.	engineered_region	1318	1397	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K091186	.	stem_loop	1329	1372	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K091186	.	engineered_region	1455	2528	.	+	0	ID=BBa_K091179;Name=BBa_K091179
BBa_K091186	.	terminator	2400	2528	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K091179
BBa_K091186	.	terminator	2400	2479	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K091186	.	engineered_region	2400	2479	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K091186	.	stem_loop	2411	2454	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K091186	.	terminator	2488	2528	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K091186	.	engineered_region	2488	2528	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K091186	.	stop_codon	2521	2521	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K091186	.	stem_loop	2495	2514	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K091186	.	polyA_site	2515	2528	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K091186	.	engineered_region	1455	2391	.	+	0	ID=BBa_K091166;Name=BBa_K091166;Parent=BBa_K091179
BBa_K091186	.	engineered_region	1593	2391	.	+	0	ID=BBa_J37033;Name=BBa_J37033;Parent=BBa_K091166
BBa_K091186	.	CDS	1611	2366	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_J37033
BBa_K091186	.	CDS	1611	2360	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K091186	.	engineered_region	1611	2366	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K091186	.	engineered_tag	2367	2391	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K091186	.	point_mutation	2102	2102	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K091186	.	point_mutation	1784	1784	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K091186	.	engineered_region	1611	1612	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K091186	.	ribosome_entry_site	1593	1604	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J37033
BBa_K091186	.	conserved_region	1597	1600	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K091186	.	promoter	1455	1584	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K091166
>BBa_K091186
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagat
ggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtg
aaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaacc
gcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtc
gcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggc
gtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatg
accaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccat
caacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatc
gcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatc
aaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatga
gggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccggg
ctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaacca
ccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaa
gggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgc
gcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaact
acgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggct
cagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacctt
cgggtgggcctttctgcgtttatatactagagacattgattatttgcacggcgtcacactttgctatgccatagcattt
ttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggc
tagctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaatta
aagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttact
cgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatat
tatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaata
tatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtt
tagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagat
agtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatag
caaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttg
ggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaataca
acaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacact
gatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgt
tttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata