LOCUS BBa_K091189 4981 bp DNA linear 22-Dec-2024 DEFINITION pLas/cI+pTet/Mnt+TetR+LasR ACCESSION BBa_K091189 VERSION BBa_K091189.1 FEATURES Location/Qualifiers promoter 140..145 /label=-10 promoter 117..122 /label=-35 misc_feature 147..164 /label=cI OR1 misc_feature 46..63 /label=plasR OP1 misc_feature 106..126 /label=plasR OP2 promoter 1..164 /label=BBa_K091143 misc_feature 177..180 /label=conserved RBS 173..184 /label=BBa_B0034 misc_feature 770..801 /label=LVA CDS 191..769 /label=luxI misc_feature 191..808 /label=BBa_C0061 misc_feature 809..833 /label=Help:Barcodes CDS 191..808 /label=BBa_C0061 misc_feature 846..849 /label=conserved RBS 842..853 /label=BBa_B0034 CDS 860..1108 /label=mnt misc_feature 954..956 /label=T misc_feature 1109..1141 /label=LVA misc_feature 1148..1172 /label=Help:Barcodes CDS 860..1147 /label=BBa_C0072 stem_loop 1192..1235 /label=stem_loop misc_feature 1181..1260 /label=BBa_B0010 terminator 1181..1260 /label=BBa_B0010 stem_loop 1276..1295 /label=T7 TE misc_feature 1302..1302 /label=stop polyA_site 1296..1309 /label=polya misc_feature 1269..1309 /label=BBa_B0012 terminator 1269..1309 /label=BBa_B0012 promoter 1364..1369 /label=-10 misc_feature 1370..1388 /label=tetR1 misc_feature 1395..1415 /label=tetR2 misc_feature 1318..1369 /label=Mnt promoter 1318..1415 /label=BBa_K091105 misc_feature 1428..1431 /label=conserved RBS 1424..1435 /label=BBa_B0034 misc_feature 2021..2052 /label=LVA CDS 1442..2020 /label=luxI misc_feature 1442..2059 /label=BBa_C0061 misc_feature 2060..2084 /label=Help:Barcodes CDS 1442..2059 /label=BBa_C0061 misc_feature 2097..2100 /label=conserved RBS 2093..2104 /label=BBa_B0034 CDS 2114..2821 /label=cI lambda misc_feature 2822..2854 /label=LVA misc_feature 2861..2885 /label=Help:Barcodes CDS 2111..2860 /label=BBa_C0051 stem_loop 2901..2920 /label=T7 TE misc_feature 2927..2927 /label=stop polyA_site 2921..2934 /label=polya misc_feature 2894..2934 /label=BBa_B0012 terminator 2894..2934 /label=BBa_B0012 stem_loop 2955..2977 /label=stem_loop misc_feature 2943..2988 /label=BBa_B0011 terminator 2943..2988 /label=BBa_B0011 promoter 2997..3126 /label=BBa_I13453 misc_feature 3139..3142 /label=conserved RBS 3135..3146 /label=BBa_B0034 CDS 3156..3772 /label=tetR misc_feature 3773..3806 /label=SsrA misc_feature 3813..3837 /label=Help:Barcodes CDS 3153..3812 /label=BBa_C0040 stem_loop 3857..3900 /label=stem_loop misc_feature 3846..3925 /label=BBa_B0010 terminator 3846..3925 /label=BBa_B0010 stem_loop 3941..3960 /label=T7 TE misc_feature 3967..3967 /label=stop polyA_site 3961..3974 /label=polya misc_feature 3934..3974 /label=BBa_B0012 terminator 3934..3974 /label=BBa_B0012 misc_feature 3985..4001 /label=lac O1 promoter 4002..4007 /label=-35 misc_feature 4008..4024 /label=lac O1 promoter 4025..4030 /label=-10 misc_feature 3983..4036 /label=BBa_R0011 promoter 3983..4036 /label=BBa_R0011 misc_feature 4050..4053 /label=conserved RBS 4046..4057 /label=BBa_B0034 CDS 4064..4780 /label=lasR misc_feature 4781..4813 /label=LVA misc_feature 4543..4543 /label=G misc_feature 4820..4844 /label=Help:Barcodes CDS 4064..4819 /label=BBa_C0079 stem_loop 4864..4907 /label=stem_loop misc_feature 4853..4932 /label=BBa_B0010 terminator 4853..4932 /label=BBa_B0010 stem_loop 4948..4967 /label=T7 TE misc_feature 4974..4974 /label=stop polyA_site 4968..4981 /label=polya misc_feature 4941..4981 /label=BBa_B0012 terminator 4941..4981 /label=BBa_B0012 ORIGIN 1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct 61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg 121 caggtttggc cgcgggttct ttttggtacc tctggcggtg ataatactag agaaagagga 181 gaaatactag atgactataa tgataaaaaa atcggatttt ttggcaattc catcggagga 241 gtataaaggt attctaagtc ttcgttatca agtgtttaag caaagacttg agtgggactt 301 agttgtagaa aataaccttg aatcagatga gtatgataac tcaaatgcag aatatattta 361 tgcttgtgat gatactgaaa atgtaagtgg atgctggcgt ttattaccta caacaggtga 421 ttatatgctg aaaagtgttt ttcctgaatt gcttggtcaa cagagtgctc ccaaagatcc 481 taatatagtc gaattaagtc gttttgctgt aggtaaaaat agctcaaaga taaataactc 541 tgctagtgaa attacaatga aactatttga agctatatat aaacacgctg ttagtcaagg 601 tattacagaa tatgtaacag taacatcaac agcaatagag cgatttttaa agcgtattaa 661 agttccttgt catcgtattg gagacaaaga aattcatgta ttaggtgata ctaaatcggt 721 tgtattgtct atgcctatta atgaacagtt taaaaaagca gtcttaaatg ctgcaaacga 781 cgaaaactac gctttagtag cttaataact ctgatagtgc tagtgtagat ctctactaga 841 gaaagaggag aaatactaga tggcccggga tgatcctcac ttcaattttc gtatgccaat 901 ggaagtaaga gagaaattga aatttagagc agaggcaaac ggacggagca tgaactctga 961 gcttttgcaa atcgtacaag atgccctaag caaaccgtca ccagtcactg ggtaccgcaa 1021 tgatgcggaa cgactcgccg atgagcagag cgagttagtg aagaagatgg tcttcgatac 1081 actgaaggat ctttataaaa aaaccaccgc tgcaaacgac gaaaactacg ctttagtagc 1141 ttaataaccc tgatagtgct agtgtagatc cctactagag ccaggcatca aataaaacga 1201 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 1261 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagagctc 1321 gaggtgagtg cacagtacta ggtccacggt gacctagatc tcctatagtt ccctatcagt 1381 gatagagaac tgtaatccct atcagtgata gagattacta gagaaagagg agaaatacta 1441 gatgactata atgataaaaa aatcggattt tttggcaatt ccatcggagg agtataaagg 1501 tattctaagt cttcgttatc aagtgtttaa gcaaagactt gagtgggact tagttgtaga 1561 aaataacctt gaatcagatg agtatgataa ctcaaatgca gaatatattt atgcttgtga 1621 tgatactgaa aatgtaagtg gatgctggcg tttattacct acaacaggtg attatatgct 1681 gaaaagtgtt tttcctgaat tgcttggtca acagagtgct cccaaagatc ctaatatagt 1741 cgaattaagt cgttttgctg taggtaaaaa tagctcaaag ataaataact ctgctagtga 1801 aattacaatg aaactatttg aagctatata taaacacgct gttagtcaag gtattacaga 1861 atatgtaaca gtaacatcaa cagcaataga gcgattttta aagcgtatta aagttccttg 1921 tcatcgtatt ggagacaaag aaattcatgt attaggtgat actaaatcgg ttgtattgtc 1981 tatgcctatt aatgaacagt ttaaaaaagc agtcttaaat gctgcaaacg acgaaaacta 2041 cgctttagta gcttaataac tctgatagtg ctagtgtaga tctctactag agaaagagga 2101 gaaatactag atgagcacaa aaaagaaacc attaacacaa gagcagcttg aggacgcacg 2161 tcgccttaaa gcaatttatg aaaaaaagaa aaatgaactt ggcttatccc aggaatctgt 2221 cgcagacaag atggggatgg ggcagtcagg cgttggtgct ttatttaatg gcatcaatgc 2281 attaaatgct tataacgccg cattgcttgc aaaaattctc aaagttagcg ttgaagaatt 2341 tagcccttca atcgccagag aaatctacga gatgtatgaa gcggttagta tgcagccgtc 2401 acttagaagt gagtatgagt accctgtttt ttctcatgtt caggcaggga tgttctcacc 2461 tgagcttaga acctttacca aaggtgatgc ggagagatgg gtaagcacaa ccaaaaaagc 2521 cagtgattct gcattctggc ttgaggttga aggtaattcc atgaccgcac caacaggctc 2581 caagccaagc tttcctgacg gaatgttaat tctcgttgac cctgagcagg ctgttgagcc 2641 aggtgatttc tgcatagcca gacttggggg tgatgagttt accttcaaga aactgatcag 2701 ggatagcggt caggtgtttt tacaaccact aaacccacag tacccaatga tcccatgcaa 2761 tgagagttgt tccgttgtgg ggaaagttat cgctagtcag tggcctgaag agacgtttgg 2821 cgctgcaaac gacgaaaact acgctttagt agcttaataa cgctgatagt gctagtgtag 2881 atcgctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag 2941 agagagaata taaaaagcca gattattaat ccggcttttt tattatttta ctagagacat 3001 tgattatttg cacggcgtca cactttgcta tgccatagca tttttatcca taagattagc 3061 ggatcctacc tgacgctttt tatcgcaact ctctactgtt tctccatacc gtttttttgg 3121 gctagctact agagaaagag gagaaatact agatgtccag attagataaa agtaaagtga 3181 ttaacagcgc attagagctg cttaatgagg tcggaatcga aggtttaaca acccgtaaac 3241 tcgcccagaa gctaggtgta gagcagccta cattgtattg gcatgtaaaa aataagcggg 3301 ctttgctcga cgccttagcc attgagatgt tagataggca ccatactcac ttttgccctt 3361 tagaagggga aagctggcaa gattttttac gtaataacgc taaaagtttt agatgtgctt 3421 tactaagtca tcgcgatgga gcaaaagtac atttaggtac acggcctaca gaaaaacagt 3481 atgaaactct cgaaaatcaa ttagcctttt tatgccaaca aggtttttca ctagagaatg 3541 cattatatgc actcagcgct gtggggcatt ttactttagg ttgcgtattg gaagatcaag 3601 agcatcaagt cgctaaagaa gaaagggaaa cacctactac tgatagtatg ccgccattat 3661 tacgacaagc tatcgaatta tttgatcacc aaggtgcaga gccagccttc ttattcggcc 3721 ttgaattgat catatgcgga ttagaaaaac aacttaaatg tgaaagtggg tccgctgcaa 3781 acgacgaaaa ctacgcttta gtagcttaat aacactgata gtgctagtgt agatcactac 3841 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 3901 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc 3961 tttctgcgtt tatatactag agaattgtga gcggataaca attgacattg tgagcggata 4021 acaagatact gagcacatac tagagaaaga ggagaaatac tagatggcct tggttgacgg 4081 ttttcttgag ctggaacgct caagtggaaa attggagtgg agcgccatcc tccagaagat 4141 ggcgagcgac cttggattct cgaagatcct gttcggcctg ttgcctaagg acagccagga 4201 ctacgagaac gccttcatcg tcggcaacta cccggccgcc tggcgcgagc attacgaccg 4261 ggctggctac gcgcgggtcg acccgacggt cagtcactgt acccagagcg tactgccgat 4321 tttctgggaa ccgtccatct accagacgcg aaagcagcac gagttcttcg aggaagcctc 4381 ggccgccggc ctggtgtatg ggctgaccat gccgctgcat ggtgctcgcg gcgaactcgg 4441 cgcgctgagc ctcagcgtgg aagcggaaaa ccgggccgag gccaaccgtt tcatagagtc 4501 ggtcctgccg accctgtgga tgctcaagga ctacgcactg caaagcggtg ccggactggc 4561 cttcgaacat ccggtcagca aaccggtggt tctgaccagc cgggagaagg aagtgttgca 4621 gtggtgcgcc atcggcaaga ccagttggga gatatcggtt atctgcaact gctcggaagc 4681 caatgtgaac ttccatatgg gaaatattcg gcggaagttc ggtgtgacct cccgccgcgt 4741 agcggccatt atggccgtta atttgggtct tattactctc gctgcaaacg acgaaaacta 4801 cgctttagta gcttaataac actgatagtg ctagtgtaga tcactactag agccaggcat 4861 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 4921 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 4981 a //