LOCUS BBa_K091190 2802 bp DNA linear 22-Dec-2024 DEFINITION plux/cI+RBS+lasI+RBS+Mnt+TT+pMnt/lac+RBS+lasI+RBS+cI+TT ACCESSION BBa_K091190 VERSION BBa_K091190.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=Lux Box promoter 20..25 /label=Lux -35 misc_feature 26..43 /label=cI OR2 promoter 44..49 /label=Lux -10 promoter 1..57 /label=BBa_K091107 misc_feature 70..73 /label=conserved RBS 66..77 /label=BBa_B0034 CDS 84..692 /label=lasI CDS 84..692 /label=BBa_C0178 misc_feature 66..692 /label=BBa_S03967 misc_feature 705..708 /label=conserved RBS 701..712 /label=BBa_B0034 CDS 719..967 /label=mnt misc_feature 813..815 /label=T misc_feature 968..1000 /label=LVA misc_feature 1007..1031 /label=Help:Barcodes CDS 719..1006 /label=BBa_C0072 misc_feature 701..1031 /label=BBa_I13025 stem_loop 1051..1094 /label=stem_loop misc_feature 1040..1119 /label=BBa_B0010 terminator 1040..1119 /label=BBa_B0010 stem_loop 1135..1154 /label=T7 TE misc_feature 1161..1161 /label=stop polyA_site 1155..1168 /label=polya misc_feature 1128..1168 /label=BBa_B0012 terminator 1128..1168 /label=BBa_B0012 terminator 1040..1168 /label=BBa_B0015 misc_feature 701..1168 /label=BBa_K091141 misc_feature 66..1168 /label=BBa_K091161 misc_feature 1..1168 /label=BBa_K091162 promoter 1223..1228 /label=-10 misc_feature 1229..1263 /label=LacI misc_feature 1177..1228 /label=Mnt promoter 1177..1263 /label=BBa_K091104 misc_feature 1276..1279 /label=conserved RBS 1272..1283 /label=BBa_B0034 CDS 1290..1898 /label=lasI CDS 1290..1898 /label=BBa_C0178 misc_feature 1272..1898 /label=BBa_S03967 misc_feature 1911..1914 /label=conserved RBS 1907..1918 /label=BBa_B0034 CDS 1928..2635 /label=cI lambda misc_feature 2636..2668 /label=LVA misc_feature 2675..2699 /label=Help:Barcodes CDS 1925..2674 /label=BBa_C0051 stem_loop 2715..2734 /label=T7 TE misc_feature 2741..2741 /label=stop polyA_site 2735..2748 /label=polya misc_feature 2708..2748 /label=BBa_B0012 terminator 2708..2748 /label=BBa_B0012 stem_loop 2769..2791 /label=stem_loop misc_feature 2757..2802 /label=BBa_B0011 terminator 2757..2802 /label=BBa_B0011 misc_feature 1907..2802 /label=BBa_P0455 misc_feature 1272..2802 /label=BBa_S04040 misc_feature 1177..2802 /label=BBa_K091188 ORIGIN 1 acctgtagga tcgtacaggt ttacgtaaca ccgtgcgtgt tgatatagtc gaataaatac 61 tagagaaaga ggagaaatac tagatgatcg ttcagatcgg tcgtcgtgaa gagttcgaca 121 aaaaactgct gggtgaaatg cacaaactgc gtgctcaggt tttcaaagaa cgtaaaggtt 181 gggacgtttc cgttatcgac gaaatggaaa tcgacggtta cgacgctctg tccccgtact 241 acatgctgat ccaggaagac accccggaag ctcaggtttt cggttgctgg cgtatcttcg 301 acaccaccgg tccgtacatg ctgaaaaaca ccttcccgga actgctgcac ggtaaagaag 361 ctccgtgctc cccgcacatc tgggaactgt cccgtttcgc tatcaactcc ggtcagaaag 421 gttccctggg tttctccgac tgcaccctgg aagctatgcg tgctctggct cgttactcct 481 tgcagaacga catccagacc ctggttaccg ttaccaccgt tggtgttgaa aaaatgatga 541 tccgtgctgg tctggacgtt tcccgtttcg gtccgcacct gaaaatcggt atcgaacgtg 601 ctgttgctct gcgtatcgaa ctgaacgcta aaacccagat cgctctgtac ggtggtgttc 661 tggttgaaca gcgtctggct gtttcctaat aatactagag aaagaggaga aatactagat 721 ggcccgggat gatcctcact tcaattttcg tatgccaatg gaagtaagag agaaattgaa 781 atttagagca gaggcaaacg gacggagcat gaactctgag cttttgcaaa tcgtacaaga 841 tgccctaagc aaaccgtcac cagtcactgg gtaccgcaat gatgcggaac gactcgccga 901 tgagcagagc gagttagtga agaagatggt cttcgataca ctgaaggatc tttataaaaa 961 aaccaccgct gcaaacgacg aaaactacgc tttagtagct taataaccct gatagtgcta 1021 gtgtagatcc ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 1081 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca 1141 ccttcgggtg ggcctttctg cgtttatata ctagagctcg aggtgagtgc acagtactag 1201 gtccacggtg acctagatct cctatagttg tgtggaattg tgagcggata acaatttcac 1261 acatactaga gaaagaggag aaatactaga tgatcgttca gatcggtcgt cgtgaagagt 1321 tcgacaaaaa actgctgggt gaaatgcaca aactgcgtgc tcaggttttc aaagaacgta 1381 aaggttggga cgtttccgtt atcgacgaaa tggaaatcga cggttacgac gctctgtccc 1441 cgtactacat gctgatccag gaagacaccc cggaagctca ggttttcggt tgctggcgta 1501 tcttcgacac caccggtccg tacatgctga aaaacacctt cccggaactg ctgcacggta 1561 aagaagctcc gtgctccccg cacatctggg aactgtcccg tttcgctatc aactccggtc 1621 agaaaggttc cctgggtttc tccgactgca ccctggaagc tatgcgtgct ctggctcgtt 1681 actccttgca gaacgacatc cagaccctgg ttaccgttac caccgttggt gttgaaaaaa 1741 tgatgatccg tgctggtctg gacgtttccc gtttcggtcc gcacctgaaa atcggtatcg 1801 aacgtgctgt tgctctgcgt atcgaactga acgctaaaac ccagatcgct ctgtacggtg 1861 gtgttctggt tgaacagcgt ctggctgttt cctaataata ctagagaaag aggagaaata 1921 ctagatgagc acaaaaaaga aaccattaac acaagagcag cttgaggacg cacgtcgcct 1981 taaagcaatt tatgaaaaaa agaaaaatga acttggctta tcccaggaat ctgtcgcaga 2041 caagatgggg atggggcagt caggcgttgg tgctttattt aatggcatca atgcattaaa 2101 tgcttataac gccgcattgc ttgcaaaaat tctcaaagtt agcgttgaag aatttagccc 2161 ttcaatcgcc agagaaatct acgagatgta tgaagcggtt agtatgcagc cgtcacttag 2221 aagtgagtat gagtaccctg ttttttctca tgttcaggca gggatgttct cacctgagct 2281 tagaaccttt accaaaggtg atgcggagag atgggtaagc acaaccaaaa aagccagtga 2341 ttctgcattc tggcttgagg ttgaaggtaa ttccatgacc gcaccaacag gctccaagcc 2401 aagctttcct gacggaatgt taattctcgt tgaccctgag caggctgttg agccaggtga 2461 tttctgcata gccagacttg ggggtgatga gtttaccttc aagaaactga tcagggatag 2521 cggtcaggtg tttttacaac cactaaaccc acagtaccca atgatcccat gcaatgagag 2581 ttgttccgtt gtggggaaag ttatcgctag tcagtggcct gaagagacgt ttggcgctgc 2641 aaacgacgaa aactacgctt tagtagctta ataacgctga tagtgctagt gtagatcgct 2701 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagagag 2761 aatataaaaa gccagattat taatccggct tttttattat tt //