LOCUS BBa_K091193 5152 bp DNA linear 25-Nov-2024 DEFINITION pLux/cI+pLac/Mnt+LacI+LuxR ACCESSION BBa_K091193 VERSION BBa_K091193.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=Lux Box promoter 20..25 /label=Lux -35 misc_feature 26..43 /label=cI OR2 promoter 44..49 /label=Lux -10 promoter 1..57 /label=BBa_K091107 misc_feature 70..73 /label=conserved RBS 66..77 /label=BBa_B0034 CDS 84..599 /label=BBa_K091109 misc_feature 612..615 /label=conserved RBS 608..619 /label=BBa_B0034 CDS 626..874 /label=mnt misc_feature 720..722 /label=T misc_feature 875..907 /label=LVA misc_feature 914..938 /label=Help:Barcodes CDS 626..913 /label=BBa_C0072 stem_loop 958..1001 /label=stem_loop misc_feature 947..1026 /label=BBa_B0010 terminator 947..1026 /label=BBa_B0010 stem_loop 1042..1061 /label=T7 TE misc_feature 1068..1068 /label=stop polyA_site 1062..1075 /label=polya misc_feature 1035..1075 /label=BBa_B0012 terminator 1035..1075 /label=BBa_B0012 promoter 1130..1135 /label=-10 misc_feature 1136..1170 /label=LacI misc_feature 1084..1135 /label=Mnt promoter 1084..1170 /label=BBa_K091104 misc_feature 1183..1186 /label=conserved RBS 1179..1190 /label=BBa_B0034 CDS 1197..1712 /label=BBa_K091109 misc_feature 1725..1728 /label=conserved RBS 1721..1732 /label=BBa_B0034 CDS 1742..2449 /label=cI lambda misc_feature 2450..2482 /label=LVA misc_feature 2489..2513 /label=Help:Barcodes CDS 1739..2488 /label=BBa_C0051 stem_loop 2529..2548 /label=T7 TE misc_feature 2555..2555 /label=stop polyA_site 2549..2562 /label=polya misc_feature 2522..2562 /label=BBa_B0012 terminator 2522..2562 /label=BBa_B0012 stem_loop 2583..2605 /label=stem_loop misc_feature 2571..2616 /label=BBa_B0011 terminator 2571..2616 /label=BBa_B0011 promoter 2625..2754 /label=BBa_I13453 misc_feature 2767..2770 /label=conserved RBS 2763..2774 /label=BBa_B0034 misc_feature 3870..3908 /label=LVA CDS 2781..3908 /label=lacI-LVA misc_feature 2781..3908 /label=BBa_C0012 misc_feature 3909..3933 /label=Help:Barcodes CDS 2781..3908 /label=BBa_C0012 stem_loop 3953..3996 /label=stem_loop misc_feature 3942..4021 /label=BBa_B0010 terminator 3942..4021 /label=BBa_B0010 stem_loop 4037..4056 /label=T7 TE misc_feature 4063..4063 /label=stop polyA_site 4057..4070 /label=polya misc_feature 4030..4070 /label=BBa_B0012 terminator 4030..4070 /label=BBa_B0012 promoter 4079..4208 /label=BBa_I13453 misc_feature 4221..4224 /label=conserved RBS 4217..4228 /label=BBa_B0034 misc_feature 4235..4236 /label=prefix misc_feature 4408..4408 /label=T misc_feature 4726..4726 /label=A CDS 4235..4984 /label=luxR misc_feature 4235..4990 /label=BBa_C0062 misc_feature 4991..5015 /label=Help:Barcodes CDS 4235..4990 /label=BBa_C0062 stem_loop 5035..5078 /label=stem_loop misc_feature 5024..5103 /label=BBa_B0010 terminator 5024..5103 /label=BBa_B0010 stem_loop 5119..5138 /label=T7 TE misc_feature 5145..5145 /label=stop polyA_site 5139..5152 /label=polya misc_feature 5112..5152 /label=BBa_B0012 terminator 5112..5152 /label=BBa_B0012 ORIGIN 1 acctgtagga tcgtacaggt ttacgtaaca ccgtgcgtgt tgatatagtc gaataaatac 61 tagagaaaga ggagaaatac tagatgccgt tgttagatag cttcacagtc gatcataccc 121 ggatggaagc gcctgctgtt cgggtggcga aaacaatgaa caccccgcat ggcgacgcaa 181 tcaccgtgtt cgatctgcgc ttctgcgtgc cgaacaaaga agtgatgcca gaaagaggga 241 tccataccct ggagcacctg tttgctggtt ttatgcgtaa ccatcttaac ggtaatggtg 301 tagagattat cgatatctcg ccaatgggct gccgcaccgg tttttatatg agtctgattg 361 gtacgccaga tgagcagcgt gttgctgatg cctggaaagc ggcaatggaa gacgtgctga 421 aagtgcagga tcagaatcag atcccggaac tgaacgtcta ccagtgtggc acttaccaga 481 tgcactcgtt gcaggaagcg caggatattg cgcgtagcat tctggaacgt gacgtacgca 541 tcaacagcaa cgaagaactg gcactgccga aagagaagtt gcaggaactg cacatctaat 601 actagagaaa gaggagaaat actagatggc ccgggatgat cctcacttca attttcgtat 661 gccaatggaa gtaagagaga aattgaaatt tagagcagag gcaaacggac ggagcatgaa 721 ctctgagctt ttgcaaatcg tacaagatgc cctaagcaaa ccgtcaccag tcactgggta 781 ccgcaatgat gcggaacgac tcgccgatga gcagagcgag ttagtgaaga agatggtctt 841 cgatacactg aaggatcttt ataaaaaaac caccgctgca aacgacgaaa actacgcttt 901 agtagcttaa taaccctgat agtgctagtg tagatcccta ctagagccag gcatcaaata 961 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac 1021 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta 1081 gagctcgagg tgagtgcaca gtactaggtc cacggtgacc tagatctcct atagttgtgt 1141 ggaattgtga gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc 1201 cgttgttaga tagcttcaca gtcgatcata cccggatgga agcgcctgct gttcgggtgg 1261 cgaaaacaat gaacaccccg catggcgacg caatcaccgt gttcgatctg cgcttctgcg 1321 tgccgaacaa agaagtgatg ccagaaagag ggatccatac cctggagcac ctgtttgctg 1381 gttttatgcg taaccatctt aacggtaatg gtgtagagat tatcgatatc tcgccaatgg 1441 gctgccgcac cggtttttat atgagtctga ttggtacgcc agatgagcag cgtgttgctg 1501 atgcctggaa agcggcaatg gaagacgtgc tgaaagtgca ggatcagaat cagatcccgg 1561 aactgaacgt ctaccagtgt ggcacttacc agatgcactc gttgcaggaa gcgcaggata 1621 ttgcgcgtag cattctggaa cgtgacgtac gcatcaacag caacgaagaa ctggcactgc 1681 cgaaagagaa gttgcaggaa ctgcacatct aatactagag aaagaggaga aatactagat 1741 gagcacaaaa aagaaaccat taacacaaga gcagcttgag gacgcacgtc gccttaaagc 1801 aatttatgaa aaaaagaaaa atgaacttgg cttatcccag gaatctgtcg cagacaagat 1861 ggggatgggg cagtcaggcg ttggtgcttt atttaatggc atcaatgcat taaatgctta 1921 taacgccgca ttgcttgcaa aaattctcaa agttagcgtt gaagaattta gcccttcaat 1981 cgccagagaa atctacgaga tgtatgaagc ggttagtatg cagccgtcac ttagaagtga 2041 gtatgagtac cctgtttttt ctcatgttca ggcagggatg ttctcacctg agcttagaac 2101 ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc aaaaaagcca gtgattctgc 2161 attctggctt gaggttgaag gtaattccat gaccgcacca acaggctcca agccaagctt 2221 tcctgacgga atgttaattc tcgttgaccc tgagcaggct gttgagccag gtgatttctg 2281 catagccaga cttgggggtg atgagtttac cttcaagaaa ctgatcaggg atagcggtca 2341 ggtgttttta caaccactaa acccacagta cccaatgatc ccatgcaatg agagttgttc 2401 cgttgtgggg aaagttatcg ctagtcagtg gcctgaagag acgtttggcg ctgcaaacga 2461 cgaaaactac gctttagtag cttaataacg ctgatagtgc tagtgtagat cgctactaga 2521 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag agagaatata 2581 aaaagccaga ttattaatcc ggctttttta ttattttact agagacattg attatttgca 2641 cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg 2701 acgcttttta tcgcaactct ctactgtttc tccataccgt ttttttgggc tagctactag 2761 agaaagagga gaaatactag atggtgaatg tgaaaccagt aacgttatac gatgtcgcag 2821 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt 2881 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc 2941 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc 3001 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg 3061 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg 3121 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc 3181 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct 3241 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg 3301 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt 3361 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc 3421 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc 3481 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc 3541 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag 3601 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac 3661 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc 3721 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg 3781 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac 3841 gacaggtttc ccgactggaa agcgggcagg ctgcaaacga cgaaaactac gctttagtag 3901 cttaataact ctgatagtgc tagtgtagat ctctactaga gccaggcatc aaataaaacg 3961 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 4021 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagac 4081 attgattatt tgcacggcgt cacactttgc tatgccatag catttttatc cataagatta 4141 gcggatccta cctgacgctt tttatcgcaa ctctctactg tttctccata ccgttttttt 4201 gggctagcta ctagagaaag aggagaaata ctagatgaaa aacataaatg ccgacgacac 4261 atacagaata attaataaaa ttaaagcttg tagaagcaat aatgatatta atcaatgctt 4321 atctgatatg actaaaatgg tacattgtga atattattta ctcgcgatca tttatcctca 4381 ttctatggtt aaatctgata tttcaatcct agataattac cctaaaaaat ggaggcaata 4441 ttatgatgac gctaatttaa taaaatatga tcctatagta gattattcta actccaatca 4501 ttcaccaatt aattggaata tatttgaaaa caatgctgta aataaaaaat ctccaaatgt 4561 aattaaagaa gcgaaaacat caggtcttat cactgggttt agtttcccta ttcatacggc 4621 taacaatggc ttcggaatgc ttagttttgc acattcagaa aaagacaact atatagatag 4681 tttattttta catgcgtgta tgaacatacc attaattgtt ccttctctag ttgataatta 4741 tcgaaaaata aatatagcaa ataataaatc aaacaacgat ttaaccaaaa gagaaaaaga 4801 atgtttagcg tgggcatgcg aaggaaaaag ctcttgggat atttcaaaaa tattaggttg 4861 cagtgagcgt actgtcactt tccatttaac caatgcgcaa atgaaactca atacaacaaa 4921 ccgctgccaa agtatttcta aagcaatttt aacaggagca attgattgcc catactttaa 4981 aaattaataa cactgatagt gctagtgtag atcactacta gagccaggca tcaaataaaa 5041 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct 5101 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta //