LOCUS       BBa_K091198             2055 bp    DNA     linear       29-Nov-2024
DEFINITION  plasRcI testing construct
ACCESSION   BBa_K091198
VERSION     BBa_K091198.1  
FEATURES             Location/Qualifiers
     promoter        140..145
                     /label=-10
     promoter        117..122
                     /label=-35
     misc_feature    147..164
                     /label=cI OR1
     misc_feature    46..63
                     /label=plasR OP1
     misc_feature    106..126
                     /label=plasR OP2
     promoter        1..164
                     /label=BBa_K091143
     RBS             173..185
                     /label=RBS-3\Weak
     RBS             179..182
                     /label=RBS
     misc_feature    173..185
                     /label=BBa_B0032
     RBS             173..185
                     /label=BBa_B0032
     CDS             192..911
                     /label=GFP protein
     CDS             192..911
                     /label=BBa_E0040
     stem_loop       931..974
                     /label=stem_loop
     misc_feature    920..999
                     /label=BBa_B0010
     terminator      920..999
                     /label=BBa_B0010
     stem_loop       1015..1034
                     /label=T7 TE
     misc_feature    1041..1041
                     /label=stop
     polyA_site      1035..1048
                     /label=polya
     misc_feature    1008..1048
                     /label=BBa_B0012
     terminator      1008..1048
                     /label=BBa_B0012
     misc_feature    1..1048
                     /label=BBa_K091169
     misc_feature    1059..1075
                     /label=lac O1
     promoter        1076..1081
                     /label=-35
     misc_feature    1082..1098
                     /label=lac O1
     promoter        1099..1104
                     /label=-10
     misc_feature    1057..1110
                     /label=BBa_R0011
     promoter        1057..1110
                     /label=BBa_R0011
     misc_feature    1124..1127
                     /label=conserved
     RBS             1120..1131
                     /label=BBa_B0034
     CDS             1138..1854
                     /label=lasR
     misc_feature    1855..1887
                     /label=LVA
     misc_feature    1617..1617
                     /label=G
     misc_feature    1894..1918
                     /label=Help:Barcodes
     CDS             1138..1893
                     /label=BBa_C0079
     misc_feature    1120..1918
                     /label=BBa_S03156
     stem_loop       1938..1981
                     /label=stem_loop
     misc_feature    1927..2006
                     /label=BBa_B0010
     terminator      1927..2006
                     /label=BBa_B0010
     stem_loop       2022..2041
                     /label=T7 TE
     misc_feature    2048..2048
                     /label=stop
     polyA_site      2042..2055
                     /label=polya
     misc_feature    2015..2055
                     /label=BBa_B0012
     terminator      2015..2055
                     /label=BBa_B0012
     terminator      1927..2055
                     /label=BBa_B0015
     misc_feature    1120..2055
                     /label=BBa_S03954
ORIGIN
        1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct
       61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg
      121 caggtttggc cgcgggttct ttttggtacc tctggcggtg ataatactag agtcacacag
      181 gaaagtacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg
      241 aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg
      301 caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat
      361 ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc
      421 atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa
      481 ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg
      541 atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc
      601 ttggacacaa attggaatac aactataact cacacaatgt atacatcatg gcagacaaac
      661 aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc
      721 aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag
      781 acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc
      841 acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat
      901 acaaataata atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
      961 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     1021 ccttcgggtg ggcctttctg cgtttatata ctagagaatt gtgagcggat aacaattgac
     1081 attgtgagcg gataacaaga tactgagcac atactagaga aagaggagaa atactagatg
     1141 gccttggttg acggttttct tgagctggaa cgctcaagtg gaaaattgga gtggagcgcc
     1201 atcctccaga agatggcgag cgaccttgga ttctcgaaga tcctgttcgg cctgttgcct
     1261 aaggacagcc aggactacga gaacgccttc atcgtcggca actacccggc cgcctggcgc
     1321 gagcattacg accgggctgg ctacgcgcgg gtcgacccga cggtcagtca ctgtacccag
     1381 agcgtactgc cgattttctg ggaaccgtcc atctaccaga cgcgaaagca gcacgagttc
     1441 ttcgaggaag cctcggccgc cggcctggtg tatgggctga ccatgccgct gcatggtgct
     1501 cgcggcgaac tcggcgcgct gagcctcagc gtggaagcgg aaaaccgggc cgaggccaac
     1561 cgtttcatag agtcggtcct gccgaccctg tggatgctca aggactacgc actgcaaagc
     1621 ggtgccggac tggccttcga acatccggtc agcaaaccgg tggttctgac cagccgggag
     1681 aaggaagtgt tgcagtggtg cgccatcggc aagaccagtt gggagatatc ggttatctgc
     1741 aactgctcgg aagccaatgt gaacttccat atgggaaata ttcggcggaa gttcggtgtg
     1801 acctcccgcc gcgtagcggc cattatggcc gttaatttgg gtcttattac tctcgctgca
     1861 aacgacgaaa actacgcttt agtagcttaa taacactgat agtgctagtg tagatcacta
     1921 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1981 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     2041 ctttctgcgt ttata
//