LOCUS BBa_K091199 3097 bp DNA linear 27-Dec-2024 DEFINITION plasR/cI overall testing construct ACCESSION BBa_K091199 VERSION BBa_K091199.1 FEATURES Location/Qualifiers promoter 140..145 /label=-10 promoter 117..122 /label=-35 misc_feature 147..164 /label=cI OR1 misc_feature 46..63 /label=plasR OP1 misc_feature 106..126 /label=plasR OP2 promoter 1..164 /label=BBa_K091143 RBS 173..185 /label=RBS-3\Weak RBS 179..182 /label=RBS misc_feature 173..185 /label=BBa_B0032 RBS 173..185 /label=BBa_B0032 CDS 192..911 /label=GFP protein CDS 192..911 /label=BBa_E0040 stem_loop 931..974 /label=stem_loop misc_feature 920..999 /label=BBa_B0010 terminator 920..999 /label=BBa_B0010 stem_loop 1015..1034 /label=T7 TE misc_feature 1041..1041 /label=stop polyA_site 1035..1048 /label=polya misc_feature 1008..1048 /label=BBa_B0012 terminator 1008..1048 /label=BBa_B0012 misc_feature 1..1048 /label=BBa_K091169 misc_feature 1059..1075 /label=lac O1 promoter 1076..1081 /label=-35 misc_feature 1082..1098 /label=lac O1 promoter 1099..1104 /label=-10 misc_feature 1057..1110 /label=BBa_R0011 promoter 1057..1110 /label=BBa_R0011 misc_feature 1124..1127 /label=conserved RBS 1120..1131 /label=BBa_B0034 CDS 1138..1854 /label=lasR misc_feature 1855..1887 /label=LVA misc_feature 1617..1617 /label=G misc_feature 1894..1918 /label=Help:Barcodes CDS 1138..1893 /label=BBa_C0079 misc_feature 1120..1918 /label=BBa_S03156 stem_loop 1938..1981 /label=stem_loop misc_feature 1927..2006 /label=BBa_B0010 terminator 1927..2006 /label=BBa_B0010 stem_loop 2022..2041 /label=T7 TE misc_feature 2048..2048 /label=stop polyA_site 2042..2055 /label=polya misc_feature 2015..2055 /label=BBa_B0012 terminator 2015..2055 /label=BBa_B0012 terminator 1927..2055 /label=BBa_B0015 misc_feature 1120..2055 /label=BBa_S03954 promoter 2064..2193 /label=BBa_I13453 misc_feature 2206..2209 /label=conserved RBS 2202..2213 /label=BBa_B0034 CDS 2223..2930 /label=cI lambda misc_feature 2931..2963 /label=LVA misc_feature 2970..2994 /label=Help:Barcodes CDS 2220..2969 /label=BBa_C0051 stem_loop 3010..3029 /label=T7 TE misc_feature 3036..3036 /label=stop polyA_site 3030..3043 /label=polya misc_feature 3003..3043 /label=BBa_B0012 terminator 3003..3043 /label=BBa_B0012 stem_loop 3064..3086 /label=stem_loop misc_feature 3052..3097 /label=BBa_B0011 terminator 3052..3097 /label=BBa_B0011 misc_feature 2202..3097 /label=BBa_P0455 misc_feature 2064..3097 /label=BBa_K091171 ORIGIN 1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct 61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg 121 caggtttggc cgcgggttct ttttggtacc tctggcggtg ataatactag agtcacacag 181 gaaagtacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg 241 aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg 301 caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat 361 ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc 421 atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa 481 ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg 541 atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc 601 ttggacacaa attggaatac aactataact cacacaatgt atacatcatg gcagacaaac 661 aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc 721 aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag 781 acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc 841 acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat 901 acaaataata atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 961 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca 1021 ccttcgggtg ggcctttctg cgtttatata ctagagaatt gtgagcggat aacaattgac 1081 attgtgagcg gataacaaga tactgagcac atactagaga aagaggagaa atactagatg 1141 gccttggttg acggttttct tgagctggaa cgctcaagtg gaaaattgga gtggagcgcc 1201 atcctccaga agatggcgag cgaccttgga ttctcgaaga tcctgttcgg cctgttgcct 1261 aaggacagcc aggactacga gaacgccttc atcgtcggca actacccggc cgcctggcgc 1321 gagcattacg accgggctgg ctacgcgcgg gtcgacccga cggtcagtca ctgtacccag 1381 agcgtactgc cgattttctg ggaaccgtcc atctaccaga cgcgaaagca gcacgagttc 1441 ttcgaggaag cctcggccgc cggcctggtg tatgggctga ccatgccgct gcatggtgct 1501 cgcggcgaac tcggcgcgct gagcctcagc gtggaagcgg aaaaccgggc cgaggccaac 1561 cgtttcatag agtcggtcct gccgaccctg tggatgctca aggactacgc actgcaaagc 1621 ggtgccggac tggccttcga acatccggtc agcaaaccgg tggttctgac cagccgggag 1681 aaggaagtgt tgcagtggtg cgccatcggc aagaccagtt gggagatatc ggttatctgc 1741 aactgctcgg aagccaatgt gaacttccat atgggaaata ttcggcggaa gttcggtgtg 1801 acctcccgcc gcgtagcggc cattatggcc gttaatttgg gtcttattac tctcgctgca 1861 aacgacgaaa actacgcttt agtagcttaa taacactgat agtgctagtg tagatcacta 1921 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 1981 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 2041 ctttctgcgt ttatatacta gagacattga ttatttgcac ggcgtcacac tttgctatgc 2101 catagcattt ttatccataa gattagcgga tcctacctga cgctttttat cgcaactctc 2161 tactgtttct ccataccgtt tttttgggct agctactaga gaaagaggag aaatactaga 2221 tgagcacaaa aaagaaacca ttaacacaag agcagcttga ggacgcacgt cgccttaaag 2281 caatttatga aaaaaagaaa aatgaacttg gcttatccca ggaatctgtc gcagacaaga 2341 tggggatggg gcagtcaggc gttggtgctt tatttaatgg catcaatgca ttaaatgctt 2401 ataacgccgc attgcttgca aaaattctca aagttagcgt tgaagaattt agcccttcaa 2461 tcgccagaga aatctacgag atgtatgaag cggttagtat gcagccgtca cttagaagtg 2521 agtatgagta ccctgttttt tctcatgttc aggcagggat gttctcacct gagcttagaa 2581 cctttaccaa aggtgatgcg gagagatggg taagcacaac caaaaaagcc agtgattctg 2641 cattctggct tgaggttgaa ggtaattcca tgaccgcacc aacaggctcc aagccaagct 2701 ttcctgacgg aatgttaatt ctcgttgacc ctgagcaggc tgttgagcca ggtgatttct 2761 gcatagccag acttgggggt gatgagttta ccttcaagaa actgatcagg gatagcggtc 2821 aggtgttttt acaaccacta aacccacagt acccaatgat cccatgcaat gagagttgtt 2881 ccgttgtggg gaaagttatc gctagtcagt ggcctgaaga gacgtttggc gctgcaaacg 2941 acgaaaacta cgctttagta gcttaataac gctgatagtg ctagtgtaga tcgctactag 3001 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gagagaatat 3061 aaaaagccag attattaatc cggctttttt attattt //