LOCUS       BBa_K091200             2067 bp    DNA     linear       27-Dec-2024
DEFINITION  plsrA/cI testing construct with lambda cI repression
ACCESSION   BBa_K091200
VERSION     BBa_K091200.1  
FEATURES             Location/Qualifiers
     promoter        1..141
                     /label=BBa_K091106
     RBS             150..162
                     /label=RBS-3\Weak
     RBS             156..159
                     /label=RBS
     misc_feature    150..162
                     /label=BBa_B0032
     RBS             150..162
                     /label=BBa_B0032
     CDS             169..888
                     /label=GFP protein
     CDS             169..888
                     /label=BBa_E0040
     stem_loop       908..951
                     /label=stem_loop
     misc_feature    897..976
                     /label=BBa_B0010
     terminator      897..976
                     /label=BBa_B0010
     stem_loop       992..1011
                     /label=T7 TE
     misc_feature    1018..1018
                     /label=stop
     polyA_site      1012..1025
                     /label=polya
     misc_feature    985..1025
                     /label=BBa_B0012
     terminator      985..1025
                     /label=BBa_B0012
     misc_feature    1..1025
                     /label=BBa_K091170
     promoter        1034..1163
                     /label=BBa_I13453
     misc_feature    1176..1179
                     /label=conserved
     RBS             1172..1183
                     /label=BBa_B0034
     CDS             1193..1900
                     /label=cI lambda
     misc_feature    1901..1933
                     /label=LVA
     misc_feature    1940..1964
                     /label=Help:Barcodes
     CDS             1190..1939
                     /label=BBa_C0051
     stem_loop       1980..1999
                     /label=T7 TE
     misc_feature    2006..2006
                     /label=stop
     polyA_site      2000..2013
                     /label=polya
     misc_feature    1973..2013
                     /label=BBa_B0012
     terminator      1973..2013
                     /label=BBa_B0012
     stem_loop       2034..2056
                     /label=stem_loop
     misc_feature    2022..2067
                     /label=BBa_B0011
     terminator      2022..2067
                     /label=BBa_B0011
     misc_feature    1172..2067
                     /label=BBa_P0455
     misc_feature    1034..2067
                     /label=BBa_K091171
ORIGIN
        1 aaccgtgaaa atcaaaatag cataaattgt gatctattcg tcggaaatat gtgcaatgtc
       61 cacctaaggt tatgaacaaa ttaaaagcag aaatacattt aacaccgtgc gtgttgattt
      121 atctaacacc gtgcgtgttg atactagagt cacacaggaa agtactagat gcgtaaagga
      181 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg
      241 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt
      301 aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt cactactttc
      361 ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc
      421 aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg
      481 aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag
      541 ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac
      601 tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac
      661 ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa
      721 aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa
      781 tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta
      841 acagctgctg ggattacaca tggcatggat gaactataca aataataata ctagagccag
      901 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
      961 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     1021 ttatatacta gagacattga ttatttgcac ggcgtcacac tttgctatgc catagcattt
     1081 ttatccataa gattagcgga tcctacctga cgctttttat cgcaactctc tactgtttct
     1141 ccataccgtt tttttgggct agctactaga gaaagaggag aaatactaga tgagcacaaa
     1201 aaagaaacca ttaacacaag agcagcttga ggacgcacgt cgccttaaag caatttatga
     1261 aaaaaagaaa aatgaacttg gcttatccca ggaatctgtc gcagacaaga tggggatggg
     1321 gcagtcaggc gttggtgctt tatttaatgg catcaatgca ttaaatgctt ataacgccgc
     1381 attgcttgca aaaattctca aagttagcgt tgaagaattt agcccttcaa tcgccagaga
     1441 aatctacgag atgtatgaag cggttagtat gcagccgtca cttagaagtg agtatgagta
     1501 ccctgttttt tctcatgttc aggcagggat gttctcacct gagcttagaa cctttaccaa
     1561 aggtgatgcg gagagatggg taagcacaac caaaaaagcc agtgattctg cattctggct
     1621 tgaggttgaa ggtaattcca tgaccgcacc aacaggctcc aagccaagct ttcctgacgg
     1681 aatgttaatt ctcgttgacc ctgagcaggc tgttgagcca ggtgatttct gcatagccag
     1741 acttgggggt gatgagttta ccttcaagaa actgatcagg gatagcggtc aggtgttttt
     1801 acaaccacta aacccacagt acccaatgat cccatgcaat gagagttgtt ccgttgtggg
     1861 gaaagttatc gctagtcagt ggcctgaaga gacgtttggc gctgcaaacg acgaaaacta
     1921 cgctttagta gcttaataac gctgatagtg ctagtgtaga tcgctactag agtcacactg
     1981 gctcaccttc gggtgggcct ttctgcgttt atatactaga gagagaatat aaaaagccag
     2041 attattaatc cggctttttt attattt
//