LOCUS BBa_K091201 4941 bp DNA linear 22-Nov-2024 DEFINITION plsrAcI overall testing construct ACCESSION BBa_K091201 VERSION BBa_K091201.1 FEATURES Location/Qualifiers promoter 1..141 /label=BBa_K091106 RBS 150..162 /label=RBS-3\Weak RBS 156..159 /label=RBS misc_feature 150..162 /label=BBa_B0032 RBS 150..162 /label=BBa_B0032 CDS 169..888 /label=GFP protein CDS 169..888 /label=BBa_E0040 stem_loop 908..951 /label=stem_loop misc_feature 897..976 /label=BBa_B0010 terminator 897..976 /label=BBa_B0010 stem_loop 992..1011 /label=T7 TE misc_feature 1018..1018 /label=stop polyA_site 1012..1025 /label=polya misc_feature 985..1025 /label=BBa_B0012 terminator 985..1025 /label=BBa_B0012 misc_feature 1..1025 /label=BBa_K091170 promoter 1034..1163 /label=BBa_I13453 misc_feature 1176..1179 /label=conserved RBS 1172..1183 /label=BBa_B0034 CDS 1190..2782 /label=BBa_K091002 misc_feature 2795..2798 /label=conserved RBS 2791..2802 /label=BBa_B0034 CDS 2809..3762 /label=BBa_K091001 stem_loop 3782..3825 /label=stem_loop misc_feature 3771..3850 /label=BBa_B0010 terminator 3771..3850 /label=BBa_B0010 stem_loop 3866..3885 /label=T7 TE misc_feature 3892..3892 /label=stop polyA_site 3886..3899 /label=polya misc_feature 3859..3899 /label=BBa_B0012 terminator 3859..3899 /label=BBa_B0012 misc_feature 1034..3899 /label=BBa_K091172 promoter 3908..4037 /label=BBa_I13453 misc_feature 4050..4053 /label=conserved RBS 4046..4057 /label=BBa_B0034 CDS 4067..4774 /label=cI lambda misc_feature 4775..4807 /label=LVA misc_feature 4814..4838 /label=Help:Barcodes CDS 4064..4813 /label=BBa_C0051 stem_loop 4854..4873 /label=T7 TE misc_feature 4880..4880 /label=stop polyA_site 4874..4887 /label=polya misc_feature 4847..4887 /label=BBa_B0012 terminator 4847..4887 /label=BBa_B0012 stem_loop 4908..4930 /label=stem_loop misc_feature 4896..4941 /label=BBa_B0011 terminator 4896..4941 /label=BBa_B0011 misc_feature 4046..4941 /label=BBa_P0455 misc_feature 3908..4941 /label=BBa_K091171 ORIGIN 1 aaccgtgaaa atcaaaatag cataaattgt gatctattcg tcggaaatat gtgcaatgtc 61 cacctaaggt tatgaacaaa ttaaaagcag aaatacattt aacaccgtgc gtgttgattt 121 atctaacacc gtgcgtgttg atactagagt cacacaggaa agtactagat gcgtaaagga 181 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg 241 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt 301 aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt cactactttc 361 ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc 421 aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg 481 aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag 541 ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac 601 tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac 661 ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa 721 aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa 781 tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta 841 acagctgctg ggattacaca tggcatggat gaactataca aataataata ctagagccag 901 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 961 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 1021 ttatatacta gagacattga ttatttgcac ggcgtcacac tttgctatgc catagcattt 1081 ttatccataa gattagcgga tcctacctga cgctttttat cgcaactctc tactgtttct 1141 ccataccgtt tttttgggct agctactaga gaaagaggag aaatactaga tggctcgact 1201 ctttaccctt tcagaatcaa agtactacct gatggcgctg gatgcaggca ccggaagtat 1261 tcgggctgtg atattcgacc tggaaggcaa tcaaatagca gtgggacagg cggagtggcg 1321 gcatctggca gtaccggacg ttcctggttc tatggaattt gatctcaaca aaaactggca 1381 actggcgtgt gagtgtatgc gccaggcgct gcacaacgcc ggcatagccc cggagtatat 1441 cgctgccgtt tcggcatgtt cgatgcgtga aggcattgtt ttatataata atgaaggagc 1501 cccgatctgg gcctgcgcca atgtggatgc cagagcggca cgcgaagtta gcgaacttaa 1561 agaactgcac aacaatacct ttgaaaacga agtttatcgc gcgaccggac aaacactggc 1621 tttaagtgcc atccccagat tactttggct ggcgcaccat cgttccgata tttaccgtca 1681 ggcatcaacc atcaccatga tcagcgactg gctggcctat atgctcagcg gcgaactggc 1741 ggtggatccc tctaacgctg gcaccacggg acttcttgat ctaaccaccc gtgactggaa 1801 acctgcattg ctggatatgg ctggcctacg tgccgatatt ctttctcctg tcaaagaaac 1861 cggcacattg ctgggcgtgg taagttcaca agcggcggaa ctctgcggtc tgaaggcggg 1921 cactccggtg gtcgttggag gaggcgacgt gcagcttggt tgccttgggt taggcgttgt 1981 gcgtccggca caaaccgcgg ttcttggcgg cacattctgg cagcaagttg taaatttagc 2041 cgcgccggtg acagacccag aaatgaacgt gcgcgttaat cctcatgtta ttcctggcat 2101 ggtacaagct gaatctataa gcttttttac cggactcacc atgcgctggt tccgcgatgc 2161 tttctgtgcc gaagaaaaac tgattgccga acgtttaggc atcgacacct atacgctgct 2221 ggaagagatg gccagtcggg tgccgcctgg gtcgtggggc gtaatgccga tcttctccga 2281 cagaatgcgc tttaaaacct ggtatcacgc tgcgccttcc tttattaact tgtccattga 2341 cccggataaa tgtaacaaag cgacattgtt ccgtgcgctg gaagaaaatg cggcgattgt 2401 atcagcgtgt aacttgcagc aaattgctga tttctcgaat attcatcctt catcgttagt 2461 ctttgcaggc ggaggttcaa aagggaaatt atggagtcaa attctcgctg atgtctcggg 2521 attacccgtc aatattccgg tggtcaaaga agccactgca ttaggatgtg ccattgcagc 2581 tggcgtcggt gccggaattt tttcatcaat ggcagaaacc ggagaacgcc tggttcgctg 2641 ggaacggacg cacacaccag acccggaaaa gcatgaactt tatcaggatt cacgcgataa 2701 gtggcaggca gtttatcagg atcagctggg gctggttgat catggactga cgacgtcgtt 2761 atggaaagcg cctgggttat agtactagag aaagaggaga aatactagat gacaatcaac 2821 gattcggcaa tttcagaaca gggaatgtgt gaagaagaac aggtcgcgcg gatcgcgtgg 2881 ttttactatc acgacgggct gacccagagc gagatcagcg atcgtctcgg cctgacacgt 2941 ttgaaagtgt cgcgattgct ggagaaaggg catcagtccg gcattattcg cgtacagatt 3001 aattctcgct ttgaaggctg tctggaatat gaaactcaat tacgtcgtca gttttcgctg 3061 caacatgtcc gggtgatccc tgggcttgcg gatgctgatg tcggtgggcg actggggata 3121 ggcgcggcgc atatgttgat gagtttactt caaccacaac agatgctggc gattggtttt 3181 ggcgaggcaa ccatgaatac gctgcaacgc ttaagtggtt ttatttcgtc acagcaaatt 3241 cgcctggtca cgctctccgg tggcgtcggt tcttatatga cgggaatcgg gcagcttaac 3301 gcggcgtgca gtgtgaatat tattccggct ccgttgcggg catcctccgc tgacattgcc 3361 cgtacgctaa aaaatgaaaa ttgcgtcaaa gatgttctgt tagccgcgca agcagcggat 3421 gtggcgattg tcggcattgg tgctgtgagt caacaggacg atgcgacaat cattcgctcc 3481 ggttatatca gccagggcga acagttaatg attggccgaa aaggggcggt tggcgacatt 3541 ttaggctact tttttgatgc aaaaggtgac gttgtcacga atatcaaaat acataacgaa 3601 ctgattggct tacctttaag cgcgctgaag accatacccg tccgggttgg cgtggcaggg 3661 ggagaaaata aagccgaagc aattgccgct gcaatgaaag gcggttatat caacgcactg 3721 gttaccgatc aggacacagc agcggcgatt ttacgtagtt aatactagag ccaggcatca 3781 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt 3841 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat 3901 actagagaca ttgattattt gcacggcgtc acactttgct atgccatagc atttttatcc 3961 ataagattag cggatcctac ctgacgcttt ttatcgcaac tctctactgt ttctccatac 4021 cgtttttttg ggctagctac tagagaaaga ggagaaatac tagatgagca caaaaaagaa 4081 accattaaca caagagcagc ttgaggacgc acgtcgcctt aaagcaattt atgaaaaaaa 4141 gaaaaatgaa cttggcttat cccaggaatc tgtcgcagac aagatgggga tggggcagtc 4201 aggcgttggt gctttattta atggcatcaa tgcattaaat gcttataacg ccgcattgct 4261 tgcaaaaatt ctcaaagtta gcgttgaaga atttagccct tcaatcgcca gagaaatcta 4321 cgagatgtat gaagcggtta gtatgcagcc gtcacttaga agtgagtatg agtaccctgt 4381 tttttctcat gttcaggcag ggatgttctc acctgagctt agaaccttta ccaaaggtga 4441 tgcggagaga tgggtaagca caaccaaaaa agccagtgat tctgcattct ggcttgaggt 4501 tgaaggtaat tccatgaccg caccaacagg ctccaagcca agctttcctg acggaatgtt 4561 aattctcgtt gaccctgagc aggctgttga gccaggtgat ttctgcatag ccagacttgg 4621 gggtgatgag tttaccttca agaaactgat cagggatagc ggtcaggtgt ttttacaacc 4681 actaaaccca cagtacccaa tgatcccatg caatgagagt tgttccgttg tggggaaagt 4741 tatcgctagt cagtggcctg aagagacgtt tggcgctgca aacgacgaaa actacgcttt 4801 agtagcttaa taacgctgat agtgctagtg tagatcgcta ctagagtcac actggctcac 4861 cttcgggtgg gcctttctgc gtttatatac tagagagaga atataaaaag ccagattatt 4921 aatccggctt ttttattatt t //