LOCUS       BBa_K091202             2090 bp    DNA     linear       26-Nov-2024
DEFINITION  plasRcI testing construct with lambda cI repression
ACCESSION   BBa_K091202
VERSION     BBa_K091202.1  
FEATURES             Location/Qualifiers
     promoter        140..145
                     /label=-10
     promoter        117..122
                     /label=-35
     misc_feature    147..164
                     /label=cI OR1
     misc_feature    46..63
                     /label=plasR OP1
     misc_feature    106..126
                     /label=plasR OP2
     promoter        1..164
                     /label=BBa_K091143
     RBS             173..185
                     /label=RBS-3\Weak
     RBS             179..182
                     /label=RBS
     misc_feature    173..185
                     /label=BBa_B0032
     RBS             173..185
                     /label=BBa_B0032
     CDS             192..911
                     /label=GFP protein
     CDS             192..911
                     /label=BBa_E0040
     stem_loop       931..974
                     /label=stem_loop
     misc_feature    920..999
                     /label=BBa_B0010
     terminator      920..999
                     /label=BBa_B0010
     stem_loop       1015..1034
                     /label=T7 TE
     misc_feature    1041..1041
                     /label=stop
     polyA_site      1035..1048
                     /label=polya
     misc_feature    1008..1048
                     /label=BBa_B0012
     terminator      1008..1048
                     /label=BBa_B0012
     misc_feature    1..1048
                     /label=BBa_K091169
     promoter        1057..1186
                     /label=BBa_I13453
     misc_feature    1199..1202
                     /label=conserved
     RBS             1195..1206
                     /label=BBa_B0034
     CDS             1216..1923
                     /label=cI lambda
     misc_feature    1924..1956
                     /label=LVA
     misc_feature    1963..1987
                     /label=Help:Barcodes
     CDS             1213..1962
                     /label=BBa_C0051
     stem_loop       2003..2022
                     /label=T7 TE
     misc_feature    2029..2029
                     /label=stop
     polyA_site      2023..2036
                     /label=polya
     misc_feature    1996..2036
                     /label=BBa_B0012
     terminator      1996..2036
                     /label=BBa_B0012
     stem_loop       2057..2079
                     /label=stem_loop
     misc_feature    2045..2090
                     /label=BBa_B0011
     terminator      2045..2090
                     /label=BBa_B0011
     misc_feature    1195..2090
                     /label=BBa_P0455
     misc_feature    1057..2090
                     /label=BBa_K091171
ORIGIN
        1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct
       61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg
      121 caggtttggc cgcgggttct ttttggtacc tctggcggtg ataatactag agtcacacag
      181 gaaagtacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg
      241 aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg
      301 caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat
      361 ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc
      421 atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa
      481 ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg
      541 atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc
      601 ttggacacaa attggaatac aactataact cacacaatgt atacatcatg gcagacaaac
      661 aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc
      721 aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag
      781 acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc
      841 acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat
      901 acaaataata atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
      961 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     1021 ccttcgggtg ggcctttctg cgtttatata ctagagacat tgattatttg cacggcgtca
     1081 cactttgcta tgccatagca tttttatcca taagattagc ggatcctacc tgacgctttt
     1141 tatcgcaact ctctactgtt tctccatacc gtttttttgg gctagctact agagaaagag
     1201 gagaaatact agatgagcac aaaaaagaaa ccattaacac aagagcagct tgaggacgca
     1261 cgtcgcctta aagcaattta tgaaaaaaag aaaaatgaac ttggcttatc ccaggaatct
     1321 gtcgcagaca agatggggat ggggcagtca ggcgttggtg ctttatttaa tggcatcaat
     1381 gcattaaatg cttataacgc cgcattgctt gcaaaaattc tcaaagttag cgttgaagaa
     1441 tttagccctt caatcgccag agaaatctac gagatgtatg aagcggttag tatgcagccg
     1501 tcacttagaa gtgagtatga gtaccctgtt ttttctcatg ttcaggcagg gatgttctca
     1561 cctgagctta gaacctttac caaaggtgat gcggagagat gggtaagcac aaccaaaaaa
     1621 gccagtgatt ctgcattctg gcttgaggtt gaaggtaatt ccatgaccgc accaacaggc
     1681 tccaagccaa gctttcctga cggaatgtta attctcgttg accctgagca ggctgttgag
     1741 ccaggtgatt tctgcatagc cagacttggg ggtgatgagt ttaccttcaa gaaactgatc
     1801 agggatagcg gtcaggtgtt tttacaacca ctaaacccac agtacccaat gatcccatgc
     1861 aatgagagtt gttccgttgt ggggaaagtt atcgctagtc agtggcctga agagacgttt
     1921 ggcgctgcaa acgacgaaaa ctacgcttta gtagcttaat aacgctgata gtgctagtgt
     1981 agatcgctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact
     2041 agagagagaa tataaaaagc cagattatta atccggcttt tttattattt
//