LOCUS       BBa_K091203             2183 bp    DNA     linear       10-May-2024
DEFINITION  pBAD-RBS-LuxR-RBS-LacI(I12)-TT
ACCESSION   BBa_K091203
VERSION     BBa_K091203.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=BBa_I13453
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     misc_feature    157..158
                     /label=prefix
     misc_feature    330..330
                     /label=T
     misc_feature    648..648
                     /label=A
     CDS             157..906
                     /label=luxR
     misc_feature    157..912
                     /label=BBa_C0062
     misc_feature    913..937
                     /label=Help:Barcodes
     CDS             157..912
                     /label=BBa_C0062
     misc_feature    139..937
                     /label=BBa_J37033
     misc_feature    1..937
                     /label=BBa_K091166
     misc_feature    950..953
                     /label=conserved
     RBS             946..957
                     /label=BBa_B0034
     misc_feature    970..972
                     /label=Proline->Tyrosine
     CDS             964..2046
                     /label=BBa_K091122
     stem_loop       2066..2109
                     /label=stem_loop
     misc_feature    2055..2134
                     /label=BBa_B0010
     terminator      2055..2134
                     /label=BBa_B0010
     stem_loop       2150..2169
                     /label=T7 TE
     misc_feature    2176..2176
                     /label=stop
     polyA_site      2170..2183
                     /label=polya
     misc_feature    2143..2183
                     /label=BBa_B0012
     terminator      2143..2183
                     /label=BBa_B0012
     misc_feature    946..2183
                     /label=BBa_S04058
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatga aaaacataaa tgccgacgac
      181 acatacagaa taattaataa aattaaagct tgtagaagca ataatgatat taatcaatgc
      241 ttatctgata tgactaaaat ggtacattgt gaatattatt tactcgcgat catttatcct
      301 cattctatgg ttaaatctga tatttcaatc ctagataatt accctaaaaa atggaggcaa
      361 tattatgatg acgctaattt aataaaatat gatcctatag tagattattc taactccaat
      421 cattcaccaa ttaattggaa tatatttgaa aacaatgctg taaataaaaa atctccaaat
      481 gtaattaaag aagcgaaaac atcaggtctt atcactgggt ttagtttccc tattcatacg
      541 gctaacaatg gcttcggaat gcttagtttt gcacattcag aaaaagacaa ctatatagat
      601 agtttatttt tacatgcgtg tatgaacata ccattaattg ttccttctct agttgataat
      661 tatcgaaaaa taaatatagc aaataataaa tcaaacaacg atttaaccaa aagagaaaaa
      721 gaatgtttag cgtgggcatg cgaaggaaaa agctcttggg atatttcaaa aatattaggt
      781 tgcagtgagc gtactgtcac tttccattta accaatgcgc aaatgaaact caatacaaca
      841 aaccgctgcc aaagtatttc taaagcaatt ttaacaggag caattgattg cccatacttt
      901 aaaaattaat aacactgata gtgctagtgt agatcactac tagagaaaga ggagaaatac
      961 tagatgaaat atgtaacgtt atacgatgtc gcagagtatg ccggtgtctc ttatcagacc
     1021 gtttcccgcg tggtgaacca ggccagccac gtttctgcga aaacgcggga aaaagtggaa
     1081 gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa
     1141 cagtcgttgc tgattggcgt tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt
     1201 gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta
     1261 gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc
     1321 agtgggctga tcattaacta tccgctggat gaccaggatg ccattgctgt ggaagctgcc
     1381 tgcactaatg ttccggcgtt atttcttgat gtctctgacc agacacccat caacagtatt
     1441 attttctccc atgaagacgg tacgcgactg ggcgtggagc atctggtcgc attgggtcac
     1501 cagcaaatcg cgctgttagc gggcccatta agttctgtct cggcgcgtct gcgtctggct
     1561 ggctggcata aatatctcac tcgcaatcaa attcagccga tagcggaacg ggaaggcgac
     1621 tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc
     1681 actgcgatgc tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc cattaccgag
     1741 tccgggctgc gcgttggtgc ggatatctcg gtagtgggat acgacgatac cgaagacagc
     1801 tcatgttata tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc
     1861 agcgtggacc gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg
     1921 cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc
     1981 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     2041 cagtgatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     2101 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
     2161 gggtgggcct ttctgcgttt ata
//