##gff-version 3 ##sequence-region BBa_K091203 1 2183 BBa_K091203 . engineered_region 1 937 . + 0 ID=BBa_K091166;Name=BBa_K091166 BBa_K091203 . promoter 1 130 . + 0 ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K091166 BBa_K091203 . engineered_region 139 937 . + 0 ID=BBa_J37033;Name=BBa_J37033;Parent=BBa_K091166 BBa_K091203 . CDS 157 912 . + 0 ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_J37033 BBa_K091203 . engineered_region 157 912 . + 0 ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062 BBa_K091203 . engineered_tag 913 937 . + 0 ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062 BBa_K091203 . CDS 157 906 . + 0 ID=annotation1766;Name=luxR;Parent=BBa_C0062 BBa_K091203 . point_mutation 330 330 . + 0 ID=annotation1764;Name=T;Parent=BBa_C0062 BBa_K091203 . engineered_region 157 158 . + 0 ID=annotation1762;Name=prefix;Parent=BBa_C0062 BBa_K091203 . point_mutation 648 648 . + 0 ID=annotation1765;Name=A;Parent=BBa_C0062 BBa_K091203 . ribosome_entry_site 139 150 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J37033 BBa_K091203 . conserved_region 143 146 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_K091203 . engineered_region 946 2183 . + 0 ID=BBa_S04058;Name=BBa_S04058 BBa_K091203 . ribosome_entry_site 946 957 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04058 BBa_K091203 . conserved_region 950 953 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_K091203 . CDS 964 2046 . + 0 ID=BBa_K091122;Name=BBa_K091122;Parent=BBa_S04058 BBa_K091203 . sequence_feature 970 972 . + 0 ID=annotation1965746;Name=Proline->Tyrosine;Parent=BBa_K091122 BBa_K091203 . terminator 2143 2183 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S04058 BBa_K091203 . polyA_site 2170 2183 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K091203 . stem_loop 2150 2169 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K091203 . engineered_region 2143 2183 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K091203 . stop_codon 2176 2176 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K091203 . terminator 2055 2134 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S04058 BBa_K091203 . stem_loop 2066 2109 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_K091203 . engineered_region 2055 2134 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 >BBa_K091203 acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacg ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagat gaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaa tgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaat ctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcc tatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatct ccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggct tcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacatacc attaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaa agagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagc gtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaat tttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagaaa gaggagaaatactagatgaaatatgtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttccc gcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacat tcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcac gcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaac gaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatcc gctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccag acacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcacc agcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcac tcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatg ctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccg agtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgcc gttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccag gcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcct ctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgatactagag ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata