LOCUS       BBa_K091206             1966 bp    DNA     linear       22-Nov-2024
DEFINITION  Prom-LuxR-TT-Prom-LasR-TT
ACCESSION   BBa_K091206
VERSION     BBa_K091206.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    62..63
                     /label=prefix
     misc_feature    235..235
                     /label=T
     misc_feature    553..553
                     /label=A
     CDS             62..811
                     /label=luxR
     misc_feature    62..817
                     /label=BBa_C0062
     misc_feature    818..842
                     /label=Help:Barcodes
     CDS             62..817
                     /label=BBa_C0062
     stem_loop       862..905
                     /label=stem_loop
     misc_feature    851..930
                     /label=BBa_B0010
     terminator      851..930
                     /label=BBa_B0010
     stem_loop       946..965
                     /label=T7 TE
     misc_feature    972..972
                     /label=stop
     polyA_site      966..979
                     /label=polya
     misc_feature    939..979
                     /label=BBa_B0012
     terminator      939..979
                     /label=BBa_B0012
     misc_feature    44..979
                     /label=BBa_I0462
     misc_feature    1..979
                     /label=BBa_K091204
     promoter        988..1022
                     /label=BBa_J23100
     misc_feature    1035..1038
                     /label=conserved
     RBS             1031..1042
                     /label=BBa_B0034
     CDS             1049..1765
                     /label=lasR
     misc_feature    1766..1798
                     /label=LVA
     misc_feature    1528..1528
                     /label=G
     misc_feature    1805..1829
                     /label=Help:Barcodes
     CDS             1049..1804
                     /label=BBa_C0079
     misc_feature    1031..1829
                     /label=BBa_S03156
     stem_loop       1849..1892
                     /label=stem_loop
     misc_feature    1838..1917
                     /label=BBa_B0010
     terminator      1838..1917
                     /label=BBa_B0010
     stem_loop       1933..1952
                     /label=T7 TE
     misc_feature    1959..1959
                     /label=stop
     polyA_site      1953..1966
                     /label=polya
     misc_feature    1926..1966
                     /label=BBa_B0012
     terminator      1926..1966
                     /label=BBa_B0012
     terminator      1838..1966
                     /label=BBa_B0015
     misc_feature    1031..1966
                     /label=BBa_S03954
     misc_feature    988..1966
                     /label=BBa_K091205
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgaaaaac ataaatgccg acgacacata cagaataatt aataaaatta aagcttgtag
      121 aagcaataat gatattaatc aatgcttatc tgatatgact aaaatggtac attgtgaata
      181 ttatttactc gcgatcattt atcctcattc tatggttaaa tctgatattt caatcctaga
      241 taattaccct aaaaaatgga ggcaatatta tgatgacgct aatttaataa aatatgatcc
      301 tatagtagat tattctaact ccaatcattc accaattaat tggaatatat ttgaaaacaa
      361 tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg aaaacatcag gtcttatcac
      421 tgggtttagt ttccctattc atacggctaa caatggcttc ggaatgctta gttttgcaca
      481 ttcagaaaaa gacaactata tagatagttt atttttacat gcgtgtatga acataccatt
      541 aattgttcct tctctagttg ataattatcg aaaaataaat atagcaaata ataaatcaaa
      601 caacgattta accaaaagag aaaaagaatg tttagcgtgg gcatgcgaag gaaaaagctc
      661 ttgggatatt tcaaaaatat taggttgcag tgagcgtact gtcactttcc atttaaccaa
      721 tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt atttctaaag caattttaac
      781 aggagcaatt gattgcccat actttaaaaa ttaataacac tgatagtgct agtgtagatc
      841 actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      901 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
      961 gggcctttct gcgtttatat actagagttg acggctagct cagtcctagg tacagtgcta
     1021 gctactagag aaagaggaga aatactagat ggccttggtt gacggttttc ttgagctgga
     1081 acgctcaagt ggaaaattgg agtggagcgc catcctccag aagatggcga gcgaccttgg
     1141 attctcgaag atcctgttcg gcctgttgcc taaggacagc caggactacg agaacgcctt
     1201 catcgtcggc aactacccgg ccgcctggcg cgagcattac gaccgggctg gctacgcgcg
     1261 ggtcgacccg acggtcagtc actgtaccca gagcgtactg ccgattttct gggaaccgtc
     1321 catctaccag acgcgaaagc agcacgagtt cttcgaggaa gcctcggccg ccggcctggt
     1381 gtatgggctg accatgccgc tgcatggtgc tcgcggcgaa ctcggcgcgc tgagcctcag
     1441 cgtggaagcg gaaaaccggg ccgaggccaa ccgtttcata gagtcggtcc tgccgaccct
     1501 gtggatgctc aaggactacg cactgcaaag cggtgccgga ctggccttcg aacatccggt
     1561 cagcaaaccg gtggttctga ccagccggga gaaggaagtg ttgcagtggt gcgccatcgg
     1621 caagaccagt tgggagatat cggttatctg caactgctcg gaagccaatg tgaacttcca
     1681 tatgggaaat attcggcgga agttcggtgt gacctcccgc cgcgtagcgg ccattatggc
     1741 cgttaatttg ggtcttatta ctctcgctgc aaacgacgaa aactacgctt tagtagctta
     1801 ataacactga tagtgctagt gtagatcact actagagcca ggcatcaaat aaaacgaaag
     1861 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
     1921 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//