BBa_K091217
type: ComponentDefinition
persistent identity: BBa K091217
display id: BBa_K091217
version: 1
was generated by: iGEM to SBOL conversion
description: LacI I12 X86 and pLac plus GFP
created: 2009-02-12T12:00:00Z
modified: 2015-05-08T01:08:39Z
mutable provenance: ligation of existing parts
owned by: james
owned by: myers
mutable description: pTet-RBS-LacII12X86-pLac-GFP
discontinued: false
dominant: false
group u list: _191_
m user id: 0
owner id: 201
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none
star: false
creator: Malcolm Campbell
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0040
component: BBa_K091127
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
component: BBa_B0032
component: BBa_K091110
component: BBa_E0040
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_K091127
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_K091110
sequence annotation: BBa_B0032
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K091217_sequence/1

BBa_R0040
type: Component
persistent identity: component2000580
display id: component2000580
version: 1
top level: BBa_K091217
definition: p(tetR)
access: public

BBa_K091127
type: Component
persistent identity: component2000589
display id: component2000589
version: 1
top level: BBa_K091217
definition: BBa_K091127
access: public

BBa_B0034
type: Component
persistent identity: component2000586
display id: component2000586
version: 1
top level: BBa_K091217
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component2000592
display id: component2000592
version: 1
top level: BBa_K091217
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2000590
display id: component2000590
version: 1
top level: BBa_K091217
definition: BBa_B0010
access: public

BBa_B0032
type: Component
persistent identity: component2000600
display id: component2000600
version: 1
top level: BBa_K091217
definition: BBa_B0032
access: public

BBa_K091110
type: Component
persistent identity: component2000598
display id: component2000598
version: 1
top level: BBa_K091217
definition: BBa_K091110
access: public

BBa_B0010
type: Component
persistent identity: component2000604
display id: component2000604
version: 1
top level: BBa_K091217
definition: BBa_B0010
access: public

BBa_E0040
type: Component
persistent identity: component2000603
display id: component2000603
version: 1
top level: BBa_K091217
definition: GFP
access: public

BBa_B0012
type: Component
persistent identity: component2000606
display id: component2000606
version: 1
top level: BBa_K091217
definition: BBa_B0012
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2000580
display id: annotation2000580
version: 1
top level: BBa_K091217
location: https://synbiohub.org/public/igem/BBa_K091217/annotation2000580/range2000580/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K091217/annotation2000580/range2000580/1
type: Range
persistent identity: range2000580
display id: range2000580
version: 1
top level: BBa_K091217
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2000586
display id: annotation2000586
version: 1
top level: BBa_K091217
location: https://synbiohub.org/public/igem/BBa_K091217/annotation2000586/range2000586/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K091217/annotation2000586/range2000586/1
type: Range
persistent identity: range2000586
display id: range2000586
version: 1
top level: BBa_K091217
direction: direction/reverse
start: 63
end: 74
orientation: inline

BBa_K091127
type: SequenceAnnotation
persistent identity: annotation2000589
display id: annotation2000589
version: 1
top level: BBa_K091217
location: https://synbiohub.org/public/igem/BBa_K091217/annotation2000589/range2000589/1
component: BBa_K091127

https://synbiohub.org/public/igem/BBa_K091217/annotation2000589/range2000589/1
type: Range
persistent identity: range2000589
display id: range2000589
version: 1
top level: BBa_K091217
direction: direction/reverse
start: 81
end: 1163
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2000590
display id: annotation2000590
version: 1
top level: BBa_K091217
location: https://synbiohub.org/public/igem/BBa_K091217/annotation2000590/range2000590/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K091217/annotation2000590/range2000590/1
type: Range
persistent identity: range2000590
display id: range2000590
version: 1
top level: BBa_K091217
direction: direction/forward
start: 1172
end: 1251
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2000592
display id: annotation2000592
version: 1
top level: BBa_K091217
location: https://synbiohub.org/public/igem/BBa_K091217/annotation2000592/range2000592/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K091217/annotation2000592/range2000592/1
type: Range
persistent identity: range2000592
display id: range2000592
version: 1
top level: BBa_K091217
direction: direction/forward
start: 1260
end: 1300
orientation: inline

BBa_K091110
type: SequenceAnnotation
persistent identity: annotation2000598
display id: annotation2000598
version: 1
top level: BBa_K091217
location: https://synbiohub.org/public/igem/BBa_K091217/annotation2000598/range2000598/1
component: BBa_K091110

https://synbiohub.org/public/igem/BBa_K091217/annotation2000598/range2000598/1
type: Range
persistent identity: range2000598
display id: range2000598
version: 1
top level: BBa_K091217
direction: direction/reverse
start: 1309
end: 1364
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2000600
display id: annotation2000600
version: 1
top level: BBa_K091217
location: https://synbiohub.org/public/igem/BBa_K091217/annotation2000600/range2000600/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K091217/annotation2000600/range2000600/1
type: Range
persistent identity: range2000600
display id: range2000600
version: 1
top level: BBa_K091217
direction: direction/forward
start: 1373
end: 1385
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2000603
display id: annotation2000603
version: 1
top level: BBa_K091217
location: https://synbiohub.org/public/igem/BBa_K091217/annotation2000603/range2000603/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K091217/annotation2000603/range2000603/1
type: Range
persistent identity: range2000603
display id: range2000603
version: 1
top level: BBa_K091217
direction: direction/reverse
start: 1392
end: 2111
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2000604
display id: annotation2000604
version: 1
top level: BBa_K091217
location: https://synbiohub.org/public/igem/BBa_K091217/annotation2000604/range2000604/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K091217/annotation2000604/range2000604/1
type: Range
persistent identity: range2000604
display id: range2000604
version: 1
top level: BBa_K091217
direction: direction/forward
start: 2120
end: 2199
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2000606
display id: annotation2000606
version: 1
top level: BBa_K091217
location: https://synbiohub.org/public/igem/BBa_K091217/annotation2000606/range2000606/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K091217/annotation2000606/range2000606/1
type: Range
persistent identity: range2000606
display id: range2000606
version: 1
top level: BBa_K091217
direction: direction/forward
start: 2208
end: 2248
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_K091110
type: ComponentDefinition
persistent identity: BBa K091110
display id: BBa_K091110
version: 1
was generated by: iGEM to SBOL conversion
description: LacI Promoter
created: 2008-06-19T11:00:00Z
modified: 2015-05-08T01:08:37Z
mutable provenance: This promoter is a modified version of the LacI promoter
owned by: james
owned by: myers
mutable description: This is a modified version of the LacI promoter that should bind LacI tighter than the original.
discontinued: false
dominant: false
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: no special considerations
star: false
creator: Robert Cool
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K091110_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1964081
display id: annotation1964081
version: 1
top level: BBa_K091110
location: https://synbiohub.org/public/igem/BBa_K091110/annotation1964081/range1964081/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K091110/annotation1964081/range1964081/1
type: Range
persistent identity: range1964081
display id: range1964081
version: 1
top level: BBa_K091110
direction: direction/forward
start: 17
end: 22
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1964082
display id: annotation1964082
version: 1
top level: BBa_K091110
location: https://synbiohub.org/public/igem/BBa_K091110/annotation1964082/range1964082/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K091110/annotation1964082/range1964082/1
type: Range
persistent identity: range1964082
display id: range1964082
version: 1
top level: BBa_K091110
direction: direction/forward
start: 44
end: 49
orientation: inline

BBa_K091127
type: ComponentDefinition
persistent identity: BBa K091127
display id: BBa_K091127
version: 1
was generated by: iGEM to SBOL conversion
description: LacI_I12_X86
created: 2008-07-09T11:00:00Z
modified: 2015-05-08T01:08:38Z
mutable provenance: The basic sequence for the LacI gene came from Ecogene.com.
owned by: james
owned by: myers
mutable description: This protein is a variation of LacI protein and has missense mutations at its third codon (proline->tyrosine) and 61st codon (serine->leucine). This causes a 10,000 fold increase in its binding affinity for the pLac promoter.
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 2993
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: See parts K091125 and K091123 for design considerations
star: false
creator: Pallavi Penumetcha
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: proline->tyrosine
sequence annotation: serine->leucine
sequence: https://synbiohub.org/public/igem/BBa_K091127_sequence/1

proline->tyrosine
type: SequenceAnnotation
persistent identity: annotation1966462
display id: annotation1966462
version: 1
top level: BBa_K091127
location: https://synbiohub.org/public/igem/BBa_K091127/annotation1966462/range1966462/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K091127/annotation1966462/range1966462/1
type: Range
persistent identity: range1966462
display id: range1966462
version: 1
top level: BBa_K091127
direction: direction/forward
start: 7
end: 9
orientation: inline

serine->leucine
type: SequenceAnnotation
persistent identity: annotation1966464
display id: annotation1966464
version: 1
top level: BBa_K091127
location: https://synbiohub.org/public/igem/BBa_K091127/annotation1966464/range1966464/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K091127/annotation1966464/range1966464/1
type: Range
persistent identity: range1966464
display id: range1966464
version: 1
top level: BBa_K091127
direction: direction/forward
start: 181
end: 183
orientation: inline

https://synbiohub.org/public/igem/BBa_K091217_sequence/1
type: Sequence
persistent identity: BBa K091217 sequence
display id: BBa_K091217_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaatatgtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagctgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcgttgacaccatcgaatggcgcaaaacctttcgcggtatggcatgatagcgcccggtactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccaca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K091110_sequence/1
type: Sequence
persistent identity: BBa K091110 sequence
display id: BBa_K091110_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgttgacaccatcgaatggcgcaaaacctttcgcggtatggcatgatagcgcccgg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K091127_sequence/1
type: Sequence
persistent identity: BBa K091127 sequence
display id: BBa_K091127_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaatatgtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagctgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtga
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z