##gff-version 3
##sequence-region BBa_K091219 1 2248
BBa_K091219	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K091219	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K091219	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K091219	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K091219	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K091219	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K091219	.	ribosome_entry_site	1373	1385	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K091219	.	ribosome_entry_site	1373	1385	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K091219	.	ribosome_entry_site	1379	1382	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K091219	.	engineered_region	1373	1385	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K091219	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K091219	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K091219	.	CDS	81	1163	.	+	0	ID=BBa_K091127;Name=BBa_K091127
BBa_K091219	.	sequence_feature	261	263	.	+	0	ID=annotation1966464;Name=serine->leucine;Parent=BBa_K091127
BBa_K091219	.	sequence_feature	87	89	.	+	0	ID=annotation1966462;Name=proline->tyrosine;Parent=BBa_K091127
BBa_K091219	.	terminator	2208	2248	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K091219	.	stem_loop	2215	2234	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K091219	.	engineered_region	2208	2248	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K091219	.	stop_codon	2241	2241	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K091219	.	polyA_site	2235	2248	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K091219	.	terminator	1260	1300	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K091219	.	stem_loop	1267	1286	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K091219	.	engineered_region	1260	1300	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K091219	.	stop_codon	1293	1293	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K091219	.	polyA_site	1287	1300	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K091219	.	promoter	1309	1364	.	+	0	ID=BBa_K091112;Name=BBa_K091112
BBa_K091219	.	promoter	1345	1349	.	+	0	ID=annotation1964078;Name=-10;Parent=BBa_K091112
BBa_K091219	.	promoter	1320	1325	.	+	0	ID=annotation1964077;Name=-35;Parent=BBa_K091112
BBa_K091219	.	terminator	1172	1251	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K091219	.	engineered_region	1172	1251	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K091219	.	stem_loop	1183	1226	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K091219	.	terminator	2120	2199	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K091219	.	engineered_region	2120	2199	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K091219	.	stem_loop	2131	2174	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K091219	.	CDS	1392	2111	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_K091219	.	CDS	1392	2111	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
>BBa_K091219
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgaaatatgtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccag
gccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtgg
cacaacaactggcgggcaaacagctgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaat
tgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaa
gcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccagg
atgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacag
tattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctg
ttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattc
agccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcat
cgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgc
gttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatca
aacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaa
tcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttg
gccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgatactagagccaggcatcaaata
aaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcaca
ctggctcaccttcgggtgggcctttctgcgtttatatactagagagcggcatgcatttacgttgacaccacctttcgcg
gtatggcatgatagcgcccggtactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggag
ttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgc
aacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactact
ttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccg
aaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaagg
tgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaa
tacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagac
acaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcctttt
accagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttctt
gagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaata
aaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcaca
ctggctcaccttcgggtgggcctttctgcgtttata