LOCUS BBa_K091220 1349 bp DNA linear 22-Nov-2024 DEFINITION RBS+TetA(C)+TT ACCESSION BBa_K091220 VERSION BBa_K091220.1 FEATURES Location/Qualifiers RBS 1..15 /label=RBS-1\Strong RBS 8..12 /label=RBS misc_feature 1..15 /label=BBa_B0030 RBS 1..15 /label=BBa_B0030 CDS 22..1212 /label=tetA(C) forward primer_bind 22..45 /label=Fwd primer J31007 primer_bind 1193..1212 /label=Rev primer J31007 misc_feature 1210..1212 /label=stop CDS 22..1212 /label=BBa_J31007 stem_loop 1232..1275 /label=stem_loop misc_feature 1221..1300 /label=BBa_B0010 terminator 1221..1300 /label=BBa_B0010 stem_loop 1316..1335 /label=T7 TE misc_feature 1342..1342 /label=stop polyA_site 1336..1349 /label=polya misc_feature 1309..1349 /label=BBa_B0012 terminator 1309..1349 /label=BBa_B0012 ORIGIN 1 attaaagagg agaaatacta gatgaaatct aacaatgcgc tcatcgtcat cctcggcacc 61 gtcaccctgg atgctgtagg cataggcttg gttatgccgg tactgccggg cctcttgcgg 121 gatatcgtcc attccgacag catcgccagt cactatggcg tgctgctagc gctatatgcg 181 ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt tggccgccgc 241 ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat ggcgaccaca 301 cccgtcctgt ggatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt 361 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc 421 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg 481 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac 541 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg accgatgccc 601 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc 661 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg 721 gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg 781 gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggccccgc caccaaacgt 841 ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg 901 ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc 961 ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag 1021 ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcat tggaccgctg 1081 atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc atggattgta 1141 ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc 1201 acctcgacct aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg 1261 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc 1321 accttcgggt gggcctttct gcgtttata //