LOCUS       BBa_K091220             1349 bp    DNA     linear       22-Dec-2024
DEFINITION  RBS+TetA(C)+TT
ACCESSION   BBa_K091220
VERSION     BBa_K091220.1  
FEATURES             Location/Qualifiers
     RBS             1..15
                     /label=RBS-1\Strong
     RBS             8..12
                     /label=RBS
     misc_feature    1..15
                     /label=BBa_B0030
     RBS             1..15
                     /label=BBa_B0030
     CDS             22..1212
                     /label=tetA(C) forward
     primer_bind     22..45
                     /label=Fwd primer J31007
     primer_bind     1193..1212
                     /label=Rev primer J31007
     misc_feature    1210..1212
                     /label=stop
     CDS             22..1212
                     /label=BBa_J31007
     stem_loop       1232..1275
                     /label=stem_loop
     misc_feature    1221..1300
                     /label=BBa_B0010
     terminator      1221..1300
                     /label=BBa_B0010
     stem_loop       1316..1335
                     /label=T7 TE
     misc_feature    1342..1342
                     /label=stop
     polyA_site      1336..1349
                     /label=polya
     misc_feature    1309..1349
                     /label=BBa_B0012
     terminator      1309..1349
                     /label=BBa_B0012
ORIGIN
        1 attaaagagg agaaatacta gatgaaatct aacaatgcgc tcatcgtcat cctcggcacc
       61 gtcaccctgg atgctgtagg cataggcttg gttatgccgg tactgccggg cctcttgcgg
      121 gatatcgtcc attccgacag catcgccagt cactatggcg tgctgctagc gctatatgcg
      181 ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt tggccgccgc
      241 ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat ggcgaccaca
      301 cccgtcctgt ggatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
      361 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
      421 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
      481 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
      541 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg accgatgccc
      601 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc
      661 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg
      721 gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg
      781 gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggccccgc caccaaacgt
      841 ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg
      901 ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc
      961 ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag
     1021 ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcat tggaccgctg
     1081 atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc atggattgta
     1141 ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc
     1201 acctcgacct aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
     1261 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
     1321 accttcgggt gggcctttct gcgtttata
//