LOCUS       BBa_K091222             2528 bp    DNA     linear       17-May-2024
DEFINITION  RBS+LacI+RBS+TetA(C)+TT
ACCESSION   BBa_K091222
VERSION     BBa_K091222.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    1108..1146
                     /label=LVA
     CDS             19..1146
                     /label=lacI-LVA
     misc_feature    19..1146
                     /label=BBa_C0012
     misc_feature    1147..1171
                     /label=Help:Barcodes
     CDS             19..1146
                     /label=BBa_C0012
     misc_feature    1..1171
                     /label=BBa_S0100
     RBS             1180..1194
                     /label=RBS-1\Strong
     RBS             1187..1191
                     /label=RBS
     misc_feature    1180..1194
                     /label=BBa_B0030
     RBS             1180..1194
                     /label=BBa_B0030
     CDS             1201..2391
                     /label=tetA(C) forward
     primer_bind     1201..1224
                     /label=Fwd primer J31007
     primer_bind     2372..2391
                     /label=Rev primer J31007
     misc_feature    2389..2391
                     /label=stop
     CDS             1201..2391
                     /label=BBa_J31007
     stem_loop       2411..2454
                     /label=stem_loop
     misc_feature    2400..2479
                     /label=BBa_B0010
     terminator      2400..2479
                     /label=BBa_B0010
     stem_loop       2495..2514
                     /label=T7 TE
     misc_feature    2521..2521
                     /label=stop
     polyA_site      2515..2528
                     /label=polya
     misc_feature    2488..2528
                     /label=BBa_B0012
     terminator      2488..2528
                     /label=BBa_B0012
     misc_feature    1180..2528
                     /label=BBa_K091220
ORIGIN
        1 aaagaggaga aatactagat ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag
       61 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct
      121 gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc
      181 gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg
      241 gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt
      301 gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg
      361 cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag
      421 gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct
      481 gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg
      541 gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct
      601 gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag
      661 ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa
      721 atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg
      781 ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg
      841 ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag
      901 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag
      961 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg
     1021 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     1081 caggtttccc gactggaaag cgggcaggct gcaaacgacg aaaactacgc tttagtagct
     1141 taataactct gatagtgcta gtgtagatct ctactagaga ttaaagagga gaaatactag
     1201 atgaaatcta acaatgcgct catcgtcatc ctcggcaccg tcaccctgga tgctgtaggc
     1261 ataggcttgg ttatgccggt actgccgggc ctcttgcggg atatcgtcca ttccgacagc
     1321 atcgccagtc actatggcgt gctgctagcg ctatatgcgt tgatgcaatt tctatgcgca
     1381 cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc cagtcctgct cgcttcgcta
     1441 cttggagcca ctatcgacta cgcgatcatg gcgaccacac ccgtcctgtg gatcctctac
     1501 gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc
     1561 gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc
     1621 ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat
     1681 gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta
     1741 atgcaggagt cgcataaggg agagcgtcga ccgatgccct tgagagcctt caacccagtc
     1801 agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt
     1861 atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc
     1921 tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc
     1981 ctcgctcaag ccttcgtcac tggccccgcc accaaacgtt tcggcgagaa gcaggccatt
     2041 atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc
     2101 tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg
     2161 caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc
     2221 gcggctctta ccagcctaac ttcgatcatt ggaccgctga tcgtcacggc gatttatgcc
     2281 gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct ataccttgtc
     2341 tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgaccta atactagagc
     2401 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     2461 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     2521 cgtttata
//