LOCUS       BBa_K091231             2772 bp    DNA     linear       23-Dec-2024
DEFINITION  LuxR producer and XOR gate
ACCESSION   BBa_K091231
VERSION     BBa_K091231.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..228
                     /label=prefix
     misc_feature    400..400
                     /label=T
     misc_feature    718..718
                     /label=A
     CDS             227..976
                     /label=luxR
     misc_feature    227..982
                     /label=BBa_C0062
     misc_feature    983..1007
                     /label=Help:Barcodes
     CDS             227..982
                     /label=BBa_C0062
     stem_loop       1027..1070
                     /label=stem_loop
     misc_feature    1016..1095
                     /label=BBa_B0010
     terminator      1016..1095
                     /label=BBa_B0010
     stem_loop       1111..1130
                     /label=T7 TE
     misc_feature    1137..1137
                     /label=stop
     polyA_site      1131..1144
                     /label=polya
     misc_feature    1104..1144
                     /label=BBa_B0012
     terminator      1104..1144
                     /label=BBa_B0012
     misc_feature    209..1144
                     /label=BBa_I0462
     misc_feature    1..1144
                     /label=BBa_K109702
     CDS             1153..1830
                     /label=mRFP reverse
     primer_bind     1811..1830
                     /label=Spe mRFP F
     primer_bind     1153..1172
                     /label=Xba mRFP R
     misc_feature    1153..1830
                     /label=BBa_J31008
     RBS             1839..1853
                     /label=Reverse RBS
     RBS             1839..1853
                     /label=BBa_J44001
     promoter        1862..1960
                     /label=BBa_K199017
     promoter        1969..2023
                     /label=BBa_K199052
     RBS             2032..2046
                     /label=RBS-1\Strong
     RBS             2039..2043
                     /label=RBS
     misc_feature    2032..2046
                     /label=BBa_B0030
     RBS             2032..2046
                     /label=BBa_B0030
     CDS             2053..2772
                     /label=GFP protein
     CDS             2053..2772
                     /label=BBa_E0040
     misc_feature    1153..2772
                     /label=BBa_K199069
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga aaaacataaa
      241 tgccgacgac acatacagaa taattaataa aattaaagct tgtagaagca ataatgatat
      301 taatcaatgc ttatctgata tgactaaaat ggtacattgt gaatattatt tactcgcgat
      361 catttatcct cattctatgg ttaaatctga tatttcaatc ctagataatt accctaaaaa
      421 atggaggcaa tattatgatg acgctaattt aataaaatat gatcctatag tagattattc
      481 taactccaat cattcaccaa ttaattggaa tatatttgaa aacaatgctg taaataaaaa
      541 atctccaaat gtaattaaag aagcgaaaac atcaggtctt atcactgggt ttagtttccc
      601 tattcatacg gctaacaatg gcttcggaat gcttagtttt gcacattcag aaaaagacaa
      661 ctatatagat agtttatttt tacatgcgtg tatgaacata ccattaattg ttccttctct
      721 agttgataat tatcgaaaaa taaatatagc aaataataaa tcaaacaacg atttaaccaa
      781 aagagaaaaa gaatgtttag cgtgggcatg cgaaggaaaa agctcttggg atatttcaaa
      841 aatattaggt tgcagtgagc gtactgtcac tttccattta accaatgcgc aaatgaaact
      901 caatacaaca aaccgctgcc aaagtatttc taaagcaatt ttaacaggag caattgattg
      961 cccatacttt aaaaattaat aacactgata gtgctagtgt agatcactac tagagccagg
     1021 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     1081 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
     1141 tatatactag agttaagcac cggtggagtg acgaccttca gcacgttcgt actgttcaac
     1201 gatggtgtag tcttcgttgt gggaggtgat gtccagtttg atgtcggttt tgtaagcacc
     1261 cggcagctga accggttttt tagccatgta ggtggtttta acttcagcgt cgtagtgacc
     1321 accgtctttc agtttcagac gcattttgat ttcacctttc agagcaccgt cttccgggta
     1381 catacgttcg gtggaagctt cccaacccat ggtttttttc tgcataaccg gaccgtcgga
     1441 cgggaagttg gtaccacgca gtttaacttt gtagatgaac tcaccgtctt gcagggagga
     1501 gtcctgggta acggtaacaa caccaccgtc ttcgaagttc ataacacgtt cccatttgaa
     1561 accttccggg aaggacagtt tcaggtagtc cgggatgtca gccgggtgtt taacgtaagc
     1621 tttggaaccg tactggaact gcggggacag gatgtcccaa gcgaacggca gcggaccacc
     1681 tttggtaact ttcagtttag cggtctgggt accttcgtac ggacgacctt caccttcacc
     1741 ttcgatttcg aactcgtgac cgttaacgga accttccata cgaactttga aacgcatgaa
     1801 ctctttgata acgtcttcgg aggaagccat tactagagtt tctcctcttt aattactaga
     1861 gtttacattt tgaaacatct atagcgataa atgaaacatc ttaaaagttt tagtatcata
     1921 ttcgtgttgg attattctgc atttttgggg agaatggact tactagagtt tattcgacta
     1981 taacaaacca ttttcttgcg taaacctgta cgatcctaca ggttactaga gattaaagag
     2041 gagaaatact agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt
     2101 gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat
     2161 gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca
     2221 tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat
     2281 catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga
     2341 actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt
     2401 gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt
     2461 cttggacaca aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa
     2521 caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt
     2581 caactagcag accattatca acaaaatact ccaattggcg atggccctgt ccttttacca
     2641 gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac
     2701 cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta
     2761 tacaaataat aa
//