##gff-version 3
##sequence-region BBa_K091232 1 1916
BBa_K091232	.	engineered_region	1	1144	.	+	0	ID=BBa_K109702;Name=BBa_K109702
BBa_K091232	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K109702
BBa_K091232	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K091232	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K091232	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K091232	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K091232	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K091232	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K091232	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K091232	.	engineered_region	209	1144	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_K109702
BBa_K091232	.	terminator	1016	1095	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K091232	.	stem_loop	1027	1070	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K091232	.	engineered_region	1016	1095	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K091232	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K091232	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K091232	.	CDS	227	982	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K091232	.	engineered_region	227	982	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K091232	.	CDS	227	976	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K091232	.	engineered_region	227	228	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K091232	.	point_mutation	400	400	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K091232	.	engineered_tag	983	1007	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K091232	.	point_mutation	718	718	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K091232	.	terminator	1104	1144	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K091232	.	stop_codon	1137	1137	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K091232	.	stem_loop	1111	1130	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K091232	.	polyA_site	1131	1144	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K091232	.	engineered_region	1104	1144	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K091232	.	engineered_region	1153	1916	.	+	0	ID=BBa_K199027;Name=BBa_K199027
BBa_K091232	.	promoter	1862	1916	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_K199027
BBa_K091232	.	non_covalent_binding_site	1862	1881	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K091232	.	start_codon	1914	1914	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K091232	.	promoter	1881	1886	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K091232	.	promoter	1903	1908	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K091232	.	engineered_region	1862	1916	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K091232	.	engineered_region	1153	1830	.	+	0	ID=BBa_J31008;Name=BBa_J31008;Parent=BBa_K199027
BBa_K091232	.	CDS	1153	1830	.	+	0	ID=annotation1911761;Name=mRFP reverse;Parent=BBa_J31008
BBa_K091232	.	primer_binding_site	1811	1830	.	+	0	ID=annotation1911764;Name=Spe mRFP F;Parent=BBa_J31008
BBa_K091232	.	primer_binding_site	1153	1172	.	+	0	ID=annotation1911765;Name=Xba mRFP R;Parent=BBa_J31008
BBa_K091232	.	ribosome_entry_site	1839	1853	.	+	0	ID=BBa_J44001;Name=BBa_J44001;Parent=BBa_K199027
BBa_K091232	.	ribosome_entry_site	1839	1853	.	+	0	ID=annotation1893199;Name=Reverse RBS;Parent=BBa_J44001
>BBa_K091232
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgaaaaacat
aaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatct
gatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatattt
caatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtaga
ttattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgta
attaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgc
ttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgt
tccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaa
gaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtca
ctttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacagg
agcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaa
taaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtca
cactggctcaccttcgggtgggcctttctgcgtttatatactagagttaagcaccggtggagtgacgaccttcagcacg
ttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatgtccagtttgatgtcggttttgtaagcacccggc
agctgaaccggttttttagccatgtaggtggttttaacttcagcgtcgtagtgaccaccgtctttcagtttcagacgca
ttttgatttcacctttcagagcaccgtcttccgggtacatacgttcggtggaagcttcccaacccatggtttttttctg
cataaccggaccgtcggacgggaagttggtaccacgcagtttaactttgtagatgaactcaccgtcttgcagggaggag
tcctgggtaacggtaacaacaccaccgtcttcgaagttcataacacgttcccatttgaaaccttccgggaaggacagtt
tcaggtagtccgggatgtcagccgggtgtttaacgtaagctttggaaccgtactggaactgcggggacaggatgtccca
agcgaacggcagcggaccacctttggtaactttcagtttagcggtctgggtaccttcgtacggacgaccttcaccttca
ccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaactttgaaacgcatgaactctttgataacgtctt
cggaggaagccattactagagtttctcctctttaattactagagacctgtaggatcgtacaggtttacgcaagaaaatg
gtttgttatagtcgaataaa