BBa_J3102
1
BBa_J3102
pBad : RBS
2006-07-20T11:00:00Z
2015-08-31T04:08:45Z
These parts are from the Resgistry 2006.
This part contains the promoter BBA_I13453 and the RBS BBA_B0030. There is an XbaI-SpeI mixed site between the two parts.
false
true
_61_
0
919
61
Not in stock
false
We neede to consider how to liagate the parts so that it did not contain restricttion sites between the parts.
false
Davidson and Missouri Western
component1886263
1
BBa_I13453
component1886265
1
BBa_B0030
annotation1886263
1
BBa_I13453
range1886263
1
1
130
annotation1886265
1
BBa_B0030
range1886265
1
139
153
BBa_I13453
1
BBa_I13453
Pbad promoter
2005-05-24T11:00:00Z
2015-08-31T04:07:34Z
Released HQ 2013
PBad promoter from I0500 without AraC.
false
false
_11_
0
253
6
In stock
false
true
jkm
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
annotation1701
1
RBS-1\Strong
range1701
1
1
15
BBa_K091234
1
BBa_K091234
pBad-RBS-EC86RT
2010-12-07T12:00:00Z
2015-05-08T01:08:39Z
J3102+I715074
This part encodes for the EC86 reverse transcriptase downstream of a weak promoter. The entire part is in the low copy plasmid PSB4A3.
false
false
_191_
0
2993
9
Not in stock
false
Had to go into a low copy vector and downstream of a weak promoter
false
Pallavi Penumetcha
component2114932
1
BBa_J3102
component2114933
1
BBa_I715074
annotation2114933
1
BBa_I715074
range2114933
1
160
1125
annotation2114932
1
BBa_J3102
range2114932
1
1
153
BBa_I715074
1
BBa_I715074
Ec86 RT
2009-12-18T12:00:00Z
2015-08-31T04:07:50Z
E.coli clinical strain B
Coding region for the reverse transcriptase of the Ec86 retron
false
false
_120_
0
1761
9
Not in stock
false
This part was designed by PCR using primers with the BioBrick prefix and suffix upstream
false
Michael Waters
BBa_I715074_sequence
1
atgaacaatttgcatgacatgtctaaggcgactcgcatatctgttgaaacacttcggttgttaatctatacagctgattttcgctataggatctacactgtagaaaagaaaggcccagagaagagaatgagaaccatttaccaaccttctcgagaacttaaagccttacaaggatgggttctacgtaacattttagataaactgtcgtcatctcctttttctattggatttgaaaagcaccaatctattttgaataatgctaccccgcatattggggcaaactttatactgaatattgatttggaggattttttcccaagtttaactgctaacaaagtttttggagtgttccattctcttggttataatcgactaatatcttcagttttgacaaaaatatgttgttataaaaatctgctaccacaaggtgctccatcatcacctaaattagctaatctaatatgttctaaacttgattatcgtattcagggttatgcaggtagtcggggcttgatatatacgagatatgccgatgatctcaccttatctgcacagtctatgaaaaaggttgttaaagcacgtgattttttattttctataatcccaagtgaaggattggttattaactcaaaaaaaacttgtattagtgggcctcgtagtcagaggaaagttacaggtttagttatttcacaagagaaagttgggataggtagagaaaaatataaagaaattagagcaaagatacatcatatattttgcggtaagtcttctgagatagaacacgttaggggatggttgtcatttattttaagtgtggattcaaaaagccataggagattaataacttatattagcaaattagaaaaaaaatatggaaagaaccctttaaataaagcgaagacctaatggtcttcgttttaaaactaaagctcataggttgaaaaattgagcacttcttcgtccaac
BBa_I13453_sequence
1
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_K091234_sequence
1
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagattaaagaggagaaatactagatgaacaatttgcatgacatgtctaaggcgactcgcatatctgttgaaacacttcggttgttaatctatacagctgattttcgctataggatctacactgtagaaaagaaaggcccagagaagagaatgagaaccatttaccaaccttctcgagaacttaaagccttacaaggatgggttctacgtaacattttagataaactgtcgtcatctcctttttctattggatttgaaaagcaccaatctattttgaataatgctaccccgcatattggggcaaactttatactgaatattgatttggaggattttttcccaagtttaactgctaacaaagtttttggagtgttccattctcttggttataatcgactaatatcttcagttttgacaaaaatatgttgttataaaaatctgctaccacaaggtgctccatcatcacctaaattagctaatctaatatgttctaaacttgattatcgtattcagggttatgcaggtagtcggggcttgatatatacgagatatgccgatgatctcaccttatctgcacagtctatgaaaaaggttgttaaagcacgtgattttttattttctataatcccaagtgaaggattggttattaactcaaaaaaaacttgtattagtgggcctcgtagtcagaggaaagttacaggtttagttatttcacaagagaaagttgggataggtagagaaaaatataaagaaattagagcaaagatacatcatatattttgcggtaagtcttctgagatagaacacgttaggggatggttgtcatttattttaagtgtggattcaaaaagccataggagattaataacttatattagcaaattagaaaaaaaatatggaaagaaccctttaaataaagcgaagacctaatggtcttcgttttaaaactaaagctcataggttgaaaaattgagcacttcttcgtccaac
BBa_J3102_sequence
1
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagattaaagaggagaaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z