LOCUS BBa_K092500 1634 bp DNA linear 26-Dec-2024 DEFINITION TetR cds with RBS and Terminator + pTetR, RFP with RBS ACCESSION BBa_K092500 VERSION BBa_K092500.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 CDS 22..638 /label=tetR misc_feature 639..672 /label=SsrA misc_feature 679..703 /label=Help:Barcodes CDS 19..678 /label=BBa_C0040 stem_loop 723..766 /label=stem_loop misc_feature 712..791 /label=BBa_B0010 terminator 712..791 /label=BBa_B0010 stem_loop 807..826 /label=T7 TE misc_feature 833..833 /label=stop polyA_site 827..840 /label=polya misc_feature 800..840 /label=BBa_B0012 terminator 800..840 /label=BBa_B0012 misc_feature 849..867 /label=TetR 1 misc_feature 849..902 /label=BBa_R0040 promoter 868..873 /label=-35 misc_feature 874..892 /label=TetR 2 promoter 891..896 /label=-10 promoter 849..902 /label=BBa_R0040 misc_feature 915..918 /label=conserved RBS 911..922 /label=BBa_B0034 CDS 929..1603 /label=mrfp1 misc_feature 1610..1634 /label=Help:Barcodes CDS 929..1609 /label=BBa_E1010 ORIGIN 1 aaagaggaga aatactagat gtccagatta gataaaagta aagtgattaa cagcgcatta 61 gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta 121 ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc 181 ttagccattg agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc 241 tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc 301 gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa 361 aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc 421 agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct 481 aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc 541 gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata 601 tgcggattag aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac 661 gctttagtag cttaataaca ctgatagtgc tagtgtagat cactactaga gccaggcatc 721 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 781 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 841 tactagagtc cctatcagtg atagagattg acatccctat cagtgataga gatactgagc 901 actactagag aaagaggaga aatactagat ggcttcctcc gaagacgtta tcaaagagtt 961 catgcgtttc aaagttcgta tggaaggttc cgttaacggt cacgagttcg aaatcgaagg 1021 tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag ttaccaaagg 1081 tggtccgctg ccgttcgctt gggacatcct gtccccgcag ttccagtacg gttccaaagc 1141 ttacgttaaa cacccggctg acatcccgga ctacctgaaa ctgtccttcc cggaaggttt 1201 caaatgggaa cgtgttatga acttcgaaga cggtggtgtt gttaccgtta cccaggactc 1261 ctccctgcaa gacggtgagt tcatctacaa agttaaactg cgtggtacca acttcccgtc 1321 cgacggtccg gttatgcaga aaaaaaccat gggttgggaa gcttccaccg aacgtatgta 1381 cccggaagac ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg 1441 tcactacgac gctgaagtta aaaccaccta catggctaaa aaaccggttc agctgccggg 1501 tgcttacaaa accgacatca aactggacat cacctcccac aacgaagact acaccatcgt 1561 tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt gcttaataac gctgatagtg 1621 ctagtgtaga tcgc // LOCUS BBa_K092600 1634 bp DNA linear 26-Dec-2024 DEFINITION TetR cds with RBS and Terminator + pTetR, RFP with RBS ACCESSION BBa_K092600 VERSION BBa_K092600.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 CDS 22..638 /label=tetR misc_feature 639..672 /label=SsrA misc_feature 679..703 /label=Help:Barcodes CDS 19..703 /label=BBa_C0040 stem_loop 723..766 /label=stem_loop misc_feature 712..791 /label=BBa_B0010 terminator 712..791 /label=BBa_B0010 stem_loop 807..826 /label=T7 TE misc_feature 833..833 /label=stop polyA_site 827..840 /label=polya misc_feature 800..840 /label=BBa_B0012 terminator 800..840 /label=BBa_B0012 terminator 712..840 /label=BBa_B0015 misc_feature 1..840 /label=BBa_K092000 misc_feature 849..867 /label=TetR 1 misc_feature 849..902 /label=BBa_R0040 promoter 868..873 /label=-35 misc_feature 874..892 /label=TetR 2 promoter 891..896 /label=-10 promoter 849..902 /label=BBa_R0040 misc_feature 915..918 /label=conserved RBS 911..922 /label=BBa_B0034 CDS 929..1603 /label=mrfp1 misc_feature 1610..1634 /label=Help:Barcodes CDS 929..1634 /label=BBa_E1010 misc_feature 849..1634 /label=BBa_K092300 ORIGIN 1 aaagaggaga aatactagat gtccagatta gataaaagta aagtgattaa cagcgcatta 61 gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta 121 ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc 181 ttagccattg agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc 241 tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc 301 gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa 361 aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc 421 agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct 481 aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc 541 gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata 601 tgcggattag aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac 661 gctttagtag cttaataaca ctgatagtgc tagtgtagat cactactaga gccaggcatc 721 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 781 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 841 tactagagtc cctatcagtg atagagattg acatccctat cagtgataga gatactgagc 901 actactagag aaagaggaga aatactagat ggcttcctcc gaagacgtta tcaaagagtt 961 catgcgtttc aaagttcgta tggaaggttc cgttaacggt cacgagttcg aaatcgaagg 1021 tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag ttaccaaagg 1081 tggtccgctg ccgttcgctt gggacatcct gtccccgcag ttccagtacg gttccaaagc 1141 ttacgttaaa cacccggctg acatcccgga ctacctgaaa ctgtccttcc cggaaggttt 1201 caaatgggaa cgtgttatga acttcgaaga cggtggtgtt gttaccgtta cccaggactc 1261 ctccctgcaa gacggtgagt tcatctacaa agttaaactg cgtggtacca acttcccgtc 1321 cgacggtccg gttatgcaga aaaaaaccat gggttgggaa gcttccaccg aacgtatgta 1381 cccggaagac ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg 1441 tcactacgac gctgaagtta aaaccaccta catggctaaa aaaccggttc agctgccggg 1501 tgcttacaaa accgacatca aactggacat cacctcccac aacgaagact acaccatcgt 1561 tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt gcttaataac gctgatagtg 1621 ctagtgtaga tcgc //