BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K092900 1 BBa_K092900 RhlR controlled by a constitutive promoter, and LacI modified with a Rhl R promoter 2008-10-21T11:00:00Z 2015-05-08T01:08:40Z The LacIm part comes from part BBa_K092800, from Basu et al."A synthetic multicelluar system for programmed pattern formation", Nature. 2005 Apr 28;434(7037):1130-4 false false _193_ 0 2618 9 It's complicated true true EPF-Lausanne Team 2008 component1993096 1 BBa_B0010 component1993105 1 BBa_R0071 component1993092 1 BBa_I14033 component1993108 1 BBa_K092800 component1993094 1 BBa_B0034 component1993098 1 BBa_B0012 component1993095 1 BBa_C0171 annotation1993095 1 BBa_C0171 range1993095 1 65 793 annotation1993092 1 BBa_I14033 range1993092 1 1 38 annotation1993108 1 BBa_K092800 range1993108 1 1000 2102 annotation1993096 1 BBa_B0010 range1993096 1 802 881 annotation1993094 1 BBa_B0034 range1993094 1 47 58 annotation1993105 1 BBa_R0071 range1993105 1 939 991 annotation1993098 1 BBa_B0012 range1993098 1 890 930 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_C0171 1 rhIR rhlR repressor/activator from P. aeruginosa PA3477 (no LVA) 2004-05-26T11:00:00Z 2015-08-31T04:07:24Z Released HQ 2013 same as C0071 except no LVA tag false false _11_1_ 0 61 7 In stock false true jcbraff BBa_K092800 1 LacIm Coding sequence for LacI modified with a different rate of translation than LacI, RBS 2008-10-21T11:00:00Z 2015-05-08T01:08:40Z LacIm from Basu et al."A synthetic multicelluar system for programmed pattern formation", Nature. 2005 Apr 28;434(7037):1130-4 false false _193_ 0 2618 9 It's complicated true true EPF-Lausanne Team 2008 annotation1984080 1 B0034 range1984080 1 1 12 annotation1984081 1 LacIm range1984081 1 21 1103 BBa_I14033 1 Cat P(Cat) 2004-08-03T11:00:00Z 2015-08-31T04:07:37Z Plasmid pACYC184 Released HQ 2013 Constitutive Promoter, Medium Transcription false false _4_ 0 171 7 In stock false true Vikram Vijayan, Allen Hsu, Lawrence Fomundam annotation1026173 1 -10 range1026173 1 27 32 annotation1026172 1 -35 range1026172 1 4 9 annotation1026174 1 P(Cat) range1026174 1 1 38 BBa_R0071 1 RhlR+C4 Promoter (RhlR & C4-HSL regulated) 2004-01-24T12:00:00Z 2015-05-08T01:14:15Z <i>Pseudomonas aeruginosa</i> rhlAB promoter Released HQ 2013 Promoter activated by RhlR in concert with C4-HSL. C4-HSL is produced by RhlI, and acts as a quorum-sensing autoinducer. <p> This is the natural sequence taken from Pseudomonas aeruginosa. <p> Crosstalk: this promoter is also activated at a low level by LasR with its associated HSL. false false _1_ 0 24 7 In stock false The -10 and -35 sites are labeled as identified in [Pearson97]. <P> The part begins with the RhlR binding site, and (rather arbitrarily) ends with the first transcribed base (+1). true Ronny Krashinsky annotation297102 1 RhlR range297102 1 1 20 annotation297104 1 -10 range297104 1 42 47 annotation297103 1 -35 range297103 1 19 24 annotation297105 1 start range297105 1 53 53 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_R0071_sequence 1 tcctgtgaaatctggcagttaccgttagctttcgaattggctaaaaagtgttc BBa_B0034_sequence 1 aaagaggagaaa BBa_C0171_sequence 1 atgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatctaataa BBa_K092900_sequence 1 ggcacgtaagaggttccaactttcaccataatgaaacatactagagaaagaggagaaatactagatgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcctgtgaaatctggcagttaccgttagctttcgaattggctaaaaagtgttctactagagaaagaggagaaatactagagtcattggccagactctaagcggctcacttggcgcgctaattgcatcaggctgtccgccagggcacgcgggctcgccgtctgggtgttcggggctaacgtcgtcttgcgcttaactaagctcaccggcagtaattggttacctttaacggcttgaccttggctcagctgtaacagacgatcaacagacgtctggcctaataaacggaagtcttgcttaatcgtcgtgcttggtggaatgtagcagctagaatcctccgtgtcatcatagccaaccacgctaatgtcggcgcccacacgtaagccgctttccgtgatcgcacgcatcgcacctaaggccatttggtcattcgccactaacatggcggtcggcacaataccttcgtttaacatctgcatcgtctgctggaagccgctcatcgcgctccaatcaccctcgcgctcggcaattggttggatctggttacgggtcaggtacttgtgccaacccgctaagcgtaagcgggcgctcacgctgctcagcggaccggccagtaaggcaatctgttgatggcccagcgccactaagtgttcaacacctaagcgggtgccatcctcgtggctaaagatgatgctattaatcggggtttgatcgctcacgtccaggaacagggctggcacgttggtacacgccgcctcaaccgcgatcgcgtcttgatcgtctaatgggtaattgataattaagccgctcacacgctgggccagcaggttatgaacggcggccttgcacgcctccacaccagagcgctccaccatgctaacaacaacagacgcgcctaactggtccgcacggctcttgatggcggccacgatctggcttggggcatgtaacgctaagctgctcgtcgccacaccgattaacaggctttgcttaccggctaactgctgcgcaacacgattcgggatatagtttaattcggccatggcggcctcaaccttctcgcgggtcttggcgctcacatgagacgcttgattaacaacacggctcaccgtttggtagctcacgcccgcgtattccgccacgtcatacagggtcaccggcttcat BBa_I14033_sequence 1 ggcacgtaagaggttccaactttcaccataatgaaaca BBa_K092800_sequence 1 aaagaggagaaatactagagtcattggccagactctaagcggctcacttggcgcgctaattgcatcaggctgtccgccagggcacgcgggctcgccgtctgggtgttcggggctaacgtcgtcttgcgcttaactaagctcaccggcagtaattggttacctttaacggcttgaccttggctcagctgtaacagacgatcaacagacgtctggcctaataaacggaagtcttgcttaatcgtcgtgcttggtggaatgtagcagctagaatcctccgtgtcatcatagccaaccacgctaatgtcggcgcccacacgtaagccgctttccgtgatcgcacgcatcgcacctaaggccatttggtcattcgccactaacatggcggtcggcacaataccttcgtttaacatctgcatcgtctgctggaagccgctcatcgcgctccaatcaccctcgcgctcggcaattggttggatctggttacgggtcaggtacttgtgccaacccgctaagcgtaagcgggcgctcacgctgctcagcggaccggccagtaaggcaatctgttgatggcccagcgccactaagtgttcaacacctaagcgggtgccatcctcgtggctaaagatgatgctattaatcggggtttgatcgctcacgtccaggaacagggctggcacgttggtacacgccgcctcaaccgcgatcgcgtcttgatcgtctaatgggtaattgataattaagccgctcacacgctgggccagcaggttatgaacggcggccttgcacgcctccacaccagagcgctccaccatgctaacaacaacagacgcgcctaactggtccgcacggctcttgatggcggccacgatctggcttggggcatgtaacgctaagctgctcgtcgccacaccgattaacaggctttgcttaccggctaactgctgcgcaacacgattcgggatatagtttaattcggccatggcggcctcaaccttctcgcgggtcttggcgctcacatgagacgcttgattaacaacacggctcaccgtttggtagctcacgcccgcgtattccgccacgtcatacagggtcaccggcttcat BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z