BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K092900
1
BBa_K092900
RhlR controlled by a constitutive promoter, and LacI modified with a Rhl R promoter
2008-10-21T11:00:00Z
2015-05-08T01:08:40Z
The LacIm part comes from part BBa_K092800, from Basu et al."A synthetic multicelluar system for programmed pattern formation", Nature. 2005 Apr 28;434(7037):1130-4
false
false
_193_
0
2618
9
It's complicated
true
true
EPF-Lausanne Team 2008
component1993096
1
BBa_B0010
component1993105
1
BBa_R0071
component1993092
1
BBa_I14033
component1993108
1
BBa_K092800
component1993094
1
BBa_B0034
component1993098
1
BBa_B0012
component1993095
1
BBa_C0171
annotation1993095
1
BBa_C0171
range1993095
1
65
793
annotation1993092
1
BBa_I14033
range1993092
1
1
38
annotation1993108
1
BBa_K092800
range1993108
1
1000
2102
annotation1993096
1
BBa_B0010
range1993096
1
802
881
annotation1993094
1
BBa_B0034
range1993094
1
47
58
annotation1993105
1
BBa_R0071
range1993105
1
939
991
annotation1993098
1
BBa_B0012
range1993098
1
890
930
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation1687
1
stop
range1687
1
34
34
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_C0171
1
rhIR
rhlR repressor/activator from P. aeruginosa PA3477 (no LVA)
2004-05-26T11:00:00Z
2015-08-31T04:07:24Z
Released HQ 2013
same as C0071 except no LVA tag
false
false
_11_1_
0
61
7
In stock
false
true
jcbraff
BBa_K092800
1
LacIm
Coding sequence for LacI modified with a different rate of translation than LacI, RBS
2008-10-21T11:00:00Z
2015-05-08T01:08:40Z
LacIm from Basu et al."A synthetic multicelluar system
for programmed pattern formation", Nature. 2005 Apr 28;434(7037):1130-4
false
false
_193_
0
2618
9
It's complicated
true
true
EPF-Lausanne Team 2008
annotation1984080
1
B0034
range1984080
1
1
12
annotation1984081
1
LacIm
range1984081
1
21
1103
BBa_I14033
1
Cat
P(Cat)
2004-08-03T11:00:00Z
2015-08-31T04:07:37Z
Plasmid pACYC184
Released HQ 2013
Constitutive Promoter, Medium Transcription
false
false
_4_
0
171
7
In stock
false
true
Vikram Vijayan, Allen Hsu, Lawrence Fomundam
annotation1026173
1
-10
range1026173
1
27
32
annotation1026172
1
-35
range1026172
1
4
9
annotation1026174
1
P(Cat)
range1026174
1
1
38
BBa_R0071
1
RhlR+C4
Promoter (RhlR & C4-HSL regulated)
2004-01-24T12:00:00Z
2015-05-08T01:14:15Z
<i>Pseudomonas aeruginosa</i> rhlAB promoter
Released HQ 2013
Promoter activated by RhlR in concert with C4-HSL. C4-HSL is produced by RhlI, and acts as a quorum-sensing autoinducer.
<p>
This is the natural sequence taken from Pseudomonas aeruginosa.
<p>
Crosstalk: this promoter is also activated at a low level by LasR with its associated HSL.
false
false
_1_
0
24
7
In stock
false
The -10 and -35 sites are labeled as identified in [Pearson97].
<P>
The part begins with the RhlR binding site, and (rather arbitrarily) ends with the first transcribed base (+1).
true
Ronny Krashinsky
annotation297102
1
RhlR
range297102
1
1
20
annotation297104
1
-10
range297104
1
42
47
annotation297103
1
-35
range297103
1
19
24
annotation297105
1
start
range297105
1
53
53
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_R0071_sequence
1
tcctgtgaaatctggcagttaccgttagctttcgaattggctaaaaagtgttc
BBa_B0034_sequence
1
aaagaggagaaa
BBa_C0171_sequence
1
atgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatctaataa
BBa_K092900_sequence
1
ggcacgtaagaggttccaactttcaccataatgaaacatactagagaaagaggagaaatactagatgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcctgtgaaatctggcagttaccgttagctttcgaattggctaaaaagtgttctactagagaaagaggagaaatactagagtcattggccagactctaagcggctcacttggcgcgctaattgcatcaggctgtccgccagggcacgcgggctcgccgtctgggtgttcggggctaacgtcgtcttgcgcttaactaagctcaccggcagtaattggttacctttaacggcttgaccttggctcagctgtaacagacgatcaacagacgtctggcctaataaacggaagtcttgcttaatcgtcgtgcttggtggaatgtagcagctagaatcctccgtgtcatcatagccaaccacgctaatgtcggcgcccacacgtaagccgctttccgtgatcgcacgcatcgcacctaaggccatttggtcattcgccactaacatggcggtcggcacaataccttcgtttaacatctgcatcgtctgctggaagccgctcatcgcgctccaatcaccctcgcgctcggcaattggttggatctggttacgggtcaggtacttgtgccaacccgctaagcgtaagcgggcgctcacgctgctcagcggaccggccagtaaggcaatctgttgatggcccagcgccactaagtgttcaacacctaagcgggtgccatcctcgtggctaaagatgatgctattaatcggggtttgatcgctcacgtccaggaacagggctggcacgttggtacacgccgcctcaaccgcgatcgcgtcttgatcgtctaatgggtaattgataattaagccgctcacacgctgggccagcaggttatgaacggcggccttgcacgcctccacaccagagcgctccaccatgctaacaacaacagacgcgcctaactggtccgcacggctcttgatggcggccacgatctggcttggggcatgtaacgctaagctgctcgtcgccacaccgattaacaggctttgcttaccggctaactgctgcgcaacacgattcgggatatagtttaattcggccatggcggcctcaaccttctcgcgggtcttggcgctcacatgagacgcttgattaacaacacggctcaccgtttggtagctcacgcccgcgtattccgccacgtcatacagggtcaccggcttcat
BBa_I14033_sequence
1
ggcacgtaagaggttccaactttcaccataatgaaaca
BBa_K092800_sequence
1
aaagaggagaaatactagagtcattggccagactctaagcggctcacttggcgcgctaattgcatcaggctgtccgccagggcacgcgggctcgccgtctgggtgttcggggctaacgtcgtcttgcgcttaactaagctcaccggcagtaattggttacctttaacggcttgaccttggctcagctgtaacagacgatcaacagacgtctggcctaataaacggaagtcttgcttaatcgtcgtgcttggtggaatgtagcagctagaatcctccgtgtcatcatagccaaccacgctaatgtcggcgcccacacgtaagccgctttccgtgatcgcacgcatcgcacctaaggccatttggtcattcgccactaacatggcggtcggcacaataccttcgtttaacatctgcatcgtctgctggaagccgctcatcgcgctccaatcaccctcgcgctcggcaattggttggatctggttacgggtcaggtacttgtgccaacccgctaagcgtaagcgggcgctcacgctgctcagcggaccggccagtaaggcaatctgttgatggcccagcgccactaagtgttcaacacctaagcgggtgccatcctcgtggctaaagatgatgctattaatcggggtttgatcgctcacgtccaggaacagggctggcacgttggtacacgccgcctcaaccgcgatcgcgtcttgatcgtctaatgggtaattgataattaagccgctcacacgctgggccagcaggttatgaacggcggccttgcacgcctccacaccagagcgctccaccatgctaacaacaacagacgcgcctaactggtccgcacggctcttgatggcggccacgatctggcttggggcatgtaacgctaagctgctcgtcgccacaccgattaacaggctttgcttaccggctaactgctgcgcaacacgattcgggatatagtttaattcggccatggcggcctcaaccttctcgcgggtcttggcgctcacatgagacgcttgattaacaacacggctcaccgtttggtagctcacgcccgcgtattccgccacgtcatacagggtcaccggcttcat
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z