LOCUS BBa_K092901 4869 bp DNA linear 27-Dec-2024 DEFINITION RhlR, PrhlR + LacI modified , PLac + TetR, pTetR + RFP and LuxI ACCESSION BBa_K092901 VERSION BBa_K092901.1 FEATURES Location/Qualifiers promoter 4..9 /label=-35 promoter 27..32 /label=-10 misc_feature 1..38 /label=P(Cat) promoter 1..38 /label=BBa_I14033 misc_feature 51..54 /label=conserved RBS 47..58 /label=BBa_B0034 CDS 65..793 /label=BBa_C0171 stem_loop 813..856 /label=stem_loop misc_feature 802..881 /label=BBa_B0010 terminator 802..881 /label=BBa_B0010 stem_loop 897..916 /label=T7 TE misc_feature 923..923 /label=stop polyA_site 917..930 /label=polya misc_feature 890..930 /label=BBa_B0012 terminator 890..930 /label=BBa_B0012 misc_feature 939..958 /label=RhlR promoter 957..962 /label=-35 promoter 980..985 /label=-10 misc_feature 991..991 /label=start promoter 939..991 /label=BBa_R0071 misc_feature 1000..1011 /label=B0034 CDS 1020..2102 /label=LacIm misc_feature 1000..2102 /label=BBa_K092800 misc_feature 1..2102 /label=BBa_K092900 stem_loop 2122..2165 /label=stem_loop misc_feature 2111..2190 /label=BBa_B0010 terminator 2111..2190 /label=BBa_B0010 stem_loop 2206..2225 /label=T7 TE misc_feature 2232..2232 /label=stop polyA_site 2226..2239 /label=polya misc_feature 2199..2239 /label=BBa_B0012 terminator 2199..2239 /label=BBa_B0012 terminator 2111..2239 /label=BBa_B0015 CDS 2248..2335 /label=end of LacI coding region (inactive) misc_feature 2248..2447 /label=BBa_R0010 misc_feature 2336..2373 /label=CAP binding site promoter 2384..2389 /label=-35 promoter 2408..2413 /label=-10 misc_feature 2413..2447 /label=LacI binding site misc_feature 2420..2420 /label=start promoter 2248..2447 /label=BBa_R0010 stem_loop 3178..3221 /label=stem_loop misc_feature 3167..3246 /label=BBa_B0010 terminator 3167..3246 /label=BBa_B0010 stem_loop 3262..3281 /label=T7 TE misc_feature 3288..3288 /label=stop polyA_site 3282..3295 /label=polya misc_feature 3255..3295 /label=BBa_B0012 terminator 3255..3295 /label=BBa_B0012 terminator 3167..3295 /label=BBa_B0015 misc_feature 2460..2463 /label=conserved RBS 2456..2467 /label=BBa_B0034 CDS 2477..3093 /label=tetR misc_feature 3094..3127 /label=SsrA misc_feature 3134..3158 /label=Help:Barcodes CDS 2474..3158 /label=BBa_C0040 stem_loop 3178..3221 /label=stem_loop misc_feature 3167..3246 /label=BBa_B0010 terminator 3167..3246 /label=BBa_B0010 stem_loop 3262..3281 /label=T7 TE misc_feature 3288..3288 /label=stop polyA_site 3282..3295 /label=polya misc_feature 3255..3295 /label=BBa_B0012 terminator 3255..3295 /label=BBa_B0012 terminator 3167..3295 /label=BBa_B0015 misc_feature 2456..3295 /label=BBa_K092000 misc_feature 3304..3322 /label=TetR 1 misc_feature 3304..3357 /label=BBa_R0040 promoter 3323..3328 /label=-35 misc_feature 3329..3347 /label=TetR 2 promoter 3346..3351 /label=-10 promoter 3304..3357 /label=BBa_R0040 misc_feature 3370..3373 /label=conserved RBS 3366..3377 /label=BBa_B0034 CDS 3384..4058 /label=mrfp1 misc_feature 4065..4089 /label=Help:Barcodes CDS 3384..4089 /label=BBa_E1010 misc_feature 3304..4089 /label=BBa_K092300 misc_feature 2456..4089 /label=BBa_K092600 misc_feature 4105..4116 /label=B0034 RBS CDS 4123..4704 /label=LuxI misc_feature 4741..4869 /label=B0015 Terminator misc_feature 4705..4740 /label=barcode misc_feature 4098..4869 /label=BBa_K092400 misc_feature 2456..4869 /label=BBa_K092700 ORIGIN 1 ggcacgtaag aggttccaac tttcaccata atgaaacata ctagagaaag aggagaaata 61 ctagatgagg aatgacggag gctttttgct gtggtgggac ggtttgcgta gcgagatgca 121 gccgatccac gacagccagg gcgtgttcgc cgtcctggaa aaggaagtgc ggcgcctggg 181 cttcgattac tacgcctatg gcgtgcgcca cacgattccc ttcacccggc cgaagaccga 241 ggtccatggc acctatccca aggcctggct ggagcgatac cagatgcaga actacggggc 301 cgtggatccg gcgatcctca acggcctgcg ctcctcggaa atggtggtct ggagcgacag 361 cctgttcgac cagagccgga tgctctggaa cgaggctcgc gattggggcc tctgtgtcgg 421 cgcgaccttg ccgatccgcg cgccgaacaa tttgctcagc gtgctttccg tggcgcgcga 481 ccagcagaac atctccagct tcgagcgcga ggaaatccgc ctgcggctgc gttgcatgat 541 cgagttgctg acccagaagc tgaccgacct ggagcatccg atgctgatgt ccaacccggt 601 ctgcctgagc catcgcgaac gcgagatcct gcaatggacc gccgacggca agagttccgg 661 ggaaatcgcc atcatcctga gcatctccga gagcacggtg aacttccacc acaagaacat 721 ccagaagaag ttcgacgcgc cgaacaagac gctggctgcc gcctacgccg cggcgctggg 781 tctcatctaa taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 841 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 901 caccttcggg tgggcctttc tgcgtttata tactagagtc ctgtgaaatc tggcagttac 961 cgttagcttt cgaattggct aaaaagtgtt ctactagaga aagaggagaa atactagagt 1021 cattggccag actctaagcg gctcacttgg cgcgctaatt gcatcaggct gtccgccagg 1081 gcacgcgggc tcgccgtctg ggtgttcggg gctaacgtcg tcttgcgctt aactaagctc 1141 accggcagta attggttacc tttaacggct tgaccttggc tcagctgtaa cagacgatca 1201 acagacgtct ggcctaataa acggaagtct tgcttaatcg tcgtgcttgg tggaatgtag 1261 cagctagaat cctccgtgtc atcatagcca accacgctaa tgtcggcgcc cacacgtaag 1321 ccgctttccg tgatcgcacg catcgcacct aaggccattt ggtcattcgc cactaacatg 1381 gcggtcggca caataccttc gtttaacatc tgcatcgtct gctggaagcc gctcatcgcg 1441 ctccaatcac cctcgcgctc ggcaattggt tggatctggt tacgggtcag gtacttgtgc 1501 caacccgcta agcgtaagcg ggcgctcacg ctgctcagcg gaccggccag taaggcaatc 1561 tgttgatggc ccagcgccac taagtgttca acacctaagc gggtgccatc ctcgtggcta 1621 aagatgatgc tattaatcgg ggtttgatcg ctcacgtcca ggaacagggc tggcacgttg 1681 gtacacgccg cctcaaccgc gatcgcgtct tgatcgtcta atgggtaatt gataattaag 1741 ccgctcacac gctgggccag caggttatga acggcggcct tgcacgcctc cacaccagag 1801 cgctccacca tgctaacaac aacagacgcg cctaactggt ccgcacggct cttgatggcg 1861 gccacgatct ggcttggggc atgtaacgct aagctgctcg tcgccacacc gattaacagg 1921 ctttgcttac cggctaactg ctgcgcaaca cgattcggga tatagtttaa ttcggccatg 1981 gcggcctcaa ccttctcgcg ggtcttggcg ctcacatgag acgcttgatt aacaacacgg 2041 ctcaccgttt ggtagctcac gcccgcgtat tccgccacgt catacagggt caccggcttc 2101 attactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 2161 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 2221 gggcctttct gcgtttatat actagagcaa tacgcaaacc gcctctcccc gcgcgttggc 2281 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 2341 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc 2401 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacatac tagagaaaga 2461 ggagaaatac tagatgtcca gattagataa aagtaaagtg attaacagcg cattagagct 2521 gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt 2581 agagcagcct acattgtatt ggcatgtaaa aaataagcgg gctttgctcg acgccttagc 2641 cattgagatg ttagataggc accatactca cttttgccct ttagaagggg aaagctggca 2701 agatttttta cgtaataacg ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg 2761 agcaaaagta catttaggta cacggcctac agaaaaacag tatgaaactc tcgaaaatca 2821 attagccttt ttatgccaac aaggtttttc actagagaat gcattatatg cactcagcgc 2881 tgtggggcat tttactttag gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga 2941 agaaagggaa acacctacta ctgatagtat gccgccatta ttacgacaag ctatcgaatt 3001 atttgatcac caaggtgcag agccagcctt cttattcggc cttgaattga tcatatgcgg 3061 attagaaaaa caacttaaat gtgaaagtgg gtccgctgca aacgacgaaa actacgcttt 3121 agtagcttaa taacactgat agtgctagtg tagatcacta ctagagccag gcatcaaata 3181 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac 3241 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta 3301 gagtccctat cagtgataga gattgacatc cctatcagtg atagagatac tgagcactac 3361 tagagaaaga ggagaaatac tagatggctt cctccgaaga cgttatcaaa gagttcatgc 3421 gtttcaaagt tcgtatggaa ggttccgtta acggtcacga gttcgaaatc gaaggtgaag 3481 gtgaaggtcg tccgtacgaa ggtacccaga ccgctaaact gaaagttacc aaaggtggtc 3541 cgctgccgtt cgcttgggac atcctgtccc cgcagttcca gtacggttcc aaagcttacg 3601 ttaaacaccc ggctgacatc ccggactacc tgaaactgtc cttcccggaa ggtttcaaat 3661 gggaacgtgt tatgaacttc gaagacggtg gtgttgttac cgttacccag gactcctccc 3721 tgcaagacgg tgagttcatc tacaaagtta aactgcgtgg taccaacttc ccgtccgacg 3781 gtccggttat gcagaaaaaa accatgggtt gggaagcttc caccgaacgt atgtacccgg 3841 aagacggtgc tctgaaaggt gaaatcaaaa tgcgtctgaa actgaaagac ggtggtcact 3901 acgacgctga agttaaaacc acctacatgg ctaaaaaacc ggttcagctg ccgggtgctt 3961 acaaaaccga catcaaactg gacatcacct cccacaacga agactacacc atcgttgaac 4021 agtacgaacg tgctgaaggt cgtcactcca ccggtgctta ataacgctga tagtgctagt 4081 gtagatcgct actagagctt gcataaagag gagaaatact agatgactat aatgataaaa 4141 aaatcggatt ttttggcaat tccatcggag gagtataaag gtattctaag tcttcgttat 4201 caagtgttta agcaaagact tgagtgggac ttagttgtag aaaataacct tgaatcagat 4261 gagtatgata actcaaatgc agaatatatt tatgcttgtg atgatactga aaatgtaagt 4321 ggatgctggc gtttattacc tacaacaggt gattatatgc tgaaaagtgt ttttcctgaa 4381 ttgcttggtc aacagagtgc tcccaaagat cctaatatag tcgaattaag tcgttttgct 4441 gtaggtaaaa atagctcaaa gataaataac tctgctagtg aaattacaat gaaactattt 4501 gaagctatat ataaacacgc tgttagtcaa ggtattacag aatatgtaac agtaacatca 4561 acagcaatag agcgattttt aaagcgtatt aaagttcctt gtcatcgtat tggagacaaa 4621 gaaattcatg tattaggtga tactaaatcg gttgtattgt ctatgcctat taatgaacag 4681 tttaaaaaag cagtcttaaa ttaataacgc tgatagtgct agtgtagatc gctactagag 4741 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 4801 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 4861 gcgtttata //