##gff-version 3
##sequence-region BBa_K092901 1 4869
BBa_K092901	.	terminator	2111	2239	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K092901	.	terminator	2199	2239	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K092901	.	engineered_region	2199	2239	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K092901	.	stop_codon	2232	2232	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K092901	.	polyA_site	2226	2239	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K092901	.	stem_loop	2206	2225	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K092901	.	terminator	2111	2190	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K092901	.	engineered_region	2111	2190	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K092901	.	stem_loop	2122	2165	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K092901	.	terminator	3167	3295	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K092901	.	terminator	3255	3295	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K092901	.	engineered_region	3255	3295	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K092901	.	stop_codon	3288	3288	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K092901	.	polyA_site	3282	3295	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K092901	.	stem_loop	3262	3281	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K092901	.	terminator	3167	3246	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K092901	.	engineered_region	3167	3246	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K092901	.	stem_loop	3178	3221	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K092901	.	engineered_region	2456	4869	.	+	0	ID=BBa_K092700;Name=BBa_K092700
BBa_K092901	.	engineered_region	2456	4089	.	+	0	ID=BBa_K092600;Name=BBa_K092600;Parent=BBa_K092700
BBa_K092901	.	engineered_region	3304	4089	.	+	0	ID=BBa_K092300;Name=BBa_K092300;Parent=BBa_K092600
BBa_K092901	.	ribosome_entry_site	3366	3377	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K092300
BBa_K092901	.	conserved_region	3370	3373	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K092901	.	promoter	3304	3357	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K092300
BBa_K092901	.	non_covalent_binding_site	3329	3347	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K092901	.	promoter	3346	3351	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K092901	.	non_covalent_binding_site	3304	3322	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K092901	.	engineered_region	3304	3357	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K092901	.	promoter	3323	3328	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K092901	.	CDS	3384	4089	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K092300
BBa_K092901	.	CDS	3384	4058	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K092901	.	engineered_tag	4065	4089	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K092901	.	engineered_region	2456	3295	.	+	0	ID=BBa_K092000;Name=BBa_K092000;Parent=BBa_K092600
BBa_K092901	.	CDS	2474	3158	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_K092000
BBa_K092901	.	engineered_tag	3134	3158	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K092901	.	CDS	2477	3093	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K092901	.	tag	3094	3127	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K092901	.	terminator	3167	3295	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K092000
BBa_K092901	.	terminator	3255	3295	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K092901	.	engineered_region	3255	3295	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K092901	.	stop_codon	3288	3288	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K092901	.	polyA_site	3282	3295	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K092901	.	stem_loop	3262	3281	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K092901	.	terminator	3167	3246	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K092901	.	engineered_region	3167	3246	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K092901	.	stem_loop	3178	3221	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K092901	.	ribosome_entry_site	2456	2467	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K092000
BBa_K092901	.	conserved_region	2460	2463	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K092901	.	engineered_region	4098	4869	.	+	0	ID=BBa_K092400;Name=BBa_K092400;Parent=BBa_K092700
BBa_K092901	.	CDS	4123	4704	.	+	0	ID=annotation1982736;Name=LuxI;Parent=BBa_K092400
BBa_K092901	.	engineered_region	4105	4116	.	+	0	ID=annotation1982735;Name=B0034 RBS;Parent=BBa_K092400
BBa_K092901	.	engineered_tag	4705	4740	.	+	0	ID=annotation1982738;Name=barcode;Parent=BBa_K092400
BBa_K092901	.	engineered_region	4741	4869	.	+	0	ID=annotation1982737;Name=B0015 Terminator;Parent=BBa_K092400
BBa_K092901	.	engineered_region	1	2102	.	+	0	ID=BBa_K092900;Name=BBa_K092900
BBa_K092901	.	promoter	1	38	.	+	0	ID=BBa_I14033;Name=BBa_I14033;Parent=BBa_K092900
BBa_K092901	.	promoter	4	9	.	+	0	ID=annotation1026172;Name=-35;Parent=BBa_I14033
BBa_K092901	.	operator	1	38	.	+	0	ID=annotation1026174;Name=P(Cat);Parent=BBa_I14033
BBa_K092901	.	promoter	27	32	.	+	0	ID=annotation1026173;Name=-10;Parent=BBa_I14033
BBa_K092901	.	CDS	65	793	.	+	0	ID=BBa_C0171;Name=BBa_C0171;Parent=BBa_K092900
BBa_K092901	.	terminator	890	930	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K092900
BBa_K092901	.	engineered_region	890	930	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K092901	.	stop_codon	923	923	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K092901	.	polyA_site	917	930	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K092901	.	stem_loop	897	916	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K092901	.	promoter	939	991	.	+	0	ID=BBa_R0071;Name=BBa_R0071;Parent=BBa_K092900
BBa_K092901	.	promoter	980	985	.	+	0	ID=annotation297104;Name=-10;Parent=BBa_R0071
BBa_K092901	.	promoter	957	962	.	+	0	ID=annotation297103;Name=-35;Parent=BBa_R0071
BBa_K092901	.	non_covalent_binding_site	939	958	.	+	0	ID=annotation297102;Name=RhlR;Parent=BBa_R0071
BBa_K092901	.	start_codon	991	991	.	+	0	ID=annotation297105;Name=start;Parent=BBa_R0071
BBa_K092901	.	engineered_region	1000	2102	.	+	0	ID=BBa_K092800;Name=BBa_K092800;Parent=BBa_K092900
BBa_K092901	.	engineered_region	1000	1011	.	+	0	ID=annotation1984080;Name=B0034;Parent=BBa_K092800
BBa_K092901	.	CDS	1020	2102	.	+	0	ID=annotation1984081;Name=LacIm;Parent=BBa_K092800
BBa_K092901	.	ribosome_entry_site	47	58	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K092900
BBa_K092901	.	conserved_region	51	54	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K092901	.	terminator	802	881	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K092900
BBa_K092901	.	engineered_region	802	881	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K092901	.	stem_loop	813	856	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K092901	.	promoter	2248	2447	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K092901	.	non_covalent_binding_site	2336	2373	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K092901	.	promoter	2408	2413	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K092901	.	non_covalent_binding_site	2413	2447	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K092901	.	promoter	2384	2389	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K092901	.	engineered_region	2248	2447	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K092901	.	CDS	2248	2335	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K092901	.	start_codon	2420	2420	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
>BBa_K092901
ggcacgtaagaggttccaactttcaccataatgaaacatactagagaaagaggagaaatactagatgaggaatgacgga
ggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctgg
aaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagac
cgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatc
ctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgagg
ctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggc
gcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacc
cagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgc
aatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccacca
caagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatctaa
taatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcg
gtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcctgtgaaa
tctggcagttaccgttagctttcgaattggctaaaaagtgttctactagagaaagaggagaaatactagagtcattggc
cagactctaagcggctcacttggcgcgctaattgcatcaggctgtccgccagggcacgcgggctcgccgtctgggtgtt
cggggctaacgtcgtcttgcgcttaactaagctcaccggcagtaattggttacctttaacggcttgaccttggctcagc
tgtaacagacgatcaacagacgtctggcctaataaacggaagtcttgcttaatcgtcgtgcttggtggaatgtagcagc
tagaatcctccgtgtcatcatagccaaccacgctaatgtcggcgcccacacgtaagccgctttccgtgatcgcacgcat
cgcacctaaggccatttggtcattcgccactaacatggcggtcggcacaataccttcgtttaacatctgcatcgtctgc
tggaagccgctcatcgcgctccaatcaccctcgcgctcggcaattggttggatctggttacgggtcaggtacttgtgcc
aacccgctaagcgtaagcgggcgctcacgctgctcagcggaccggccagtaaggcaatctgttgatggcccagcgccac
taagtgttcaacacctaagcgggtgccatcctcgtggctaaagatgatgctattaatcggggtttgatcgctcacgtcc
aggaacagggctggcacgttggtacacgccgcctcaaccgcgatcgcgtcttgatcgtctaatgggtaattgataatta
agccgctcacacgctgggccagcaggttatgaacggcggccttgcacgcctccacaccagagcgctccaccatgctaac
aacaacagacgcgcctaactggtccgcacggctcttgatggcggccacgatctggcttggggcatgtaacgctaagctg
ctcgtcgccacaccgattaacaggctttgcttaccggctaactgctgcgcaacacgattcgggatatagtttaattcgg
ccatggcggcctcaaccttctcgcgggtcttggcgctcacatgagacgcttgattaacaacacggctcaccgtttggta
gctcacgcccgcgtattccgccacgtcatacagggtcaccggcttcattactagagccaggcatcaaataaaacgaaag
gctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcac
cttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaa
tgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcatt
aggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacata
ctagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatg
aggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgt
aaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaa
ggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaa
aagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaagg
tttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagag
catcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattat
ttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatg
tgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcacta
ctagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaa
cgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgata
gagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggcttcctccga
agacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggt
gaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgctt
gggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaa
actgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggac
tcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgc
agaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaat
gcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctg
ccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaac
gtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagcttgcataaag
aggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattct
aagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtat
gataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaa
caggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcga
attaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaa
gctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaa
agcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtc
tatgcctattaatgaacagtttaaaaaagcagtcttaaattaataacgctgatagtgctagtgtagatcgctactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata