BBa_K092901
type: ComponentDefinition
persistent identity: BBa K092901
display id: BBa_K092901
version: 1
was generated by: iGEM to SBOL conversion
description: RhlR, PrhlR + LacI modified , PLac + TetR, pTetR + RFP and LuxI
created: 2008-10-21T11:00:00Z
modified: 2015-05-08T01:08:40Z
mutable provenance: Source of LacIm : Basu et al."A synthetic multicelluar system for programmed pattern formation", Nature. 2005 Apr 28;434(7037):1130-4
owned by: james
owned by: myers
mutable description: Sequence containing : - RhlR controlled by a constitutive promoter - LacI modified with a RhlR promoter - TetR controlled by a Lac Promoter - RFP controlled by a TetR promoter - LuxI is added at the end of the cds of the RFP. As no terminator was placed after the RFP, they both depends on the TetR promoter. The transcription of RFP and LuxI is inhibited when TetR is present. The production of TetR depends on the promoter you put in front of it.
discontinued: false
dominant: false
experience: experience/None
group u list: _193_
m user id: 0
owner id: 2618
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: EPF-Lausanne Team 2008
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_K092900
component: BBa_R0010
component: BBa_B0015
component: BBa_K092700
sequence annotation: BBa_K092900
sequence annotation: BBa_B0015
sequence annotation: BBa_R0010
sequence annotation: BBa_K092700
sequence: https://synbiohub.org/public/igem/BBa_K092901_sequence/1

BBa_K092900
type: Component
persistent identity: component2317915
display id: component2317915
version: 1
top level: BBa_K092901
definition: BBa_K092900
access: public

BBa_R0010
type: Component
persistent identity: component2317923
display id: component2317923
version: 1
top level: BBa_K092901
definition: LacI
access: public

BBa_B0015
type: Component
persistent identity: component2317922
display id: component2317922
version: 1
top level: BBa_K092901
definition: BBa_B0015
access: public

BBa_K092700
type: Component
persistent identity: component2317961
display id: component2317961
version: 1
top level: BBa_K092901
definition: BBa_K092700
access: public

BBa_B0015
type: Component
persistent identity: component2317939
display id: component2317939
version: 1
top level: BBa_K092901
definition: BBa_B0015
access: public

BBa_K092900
type: SequenceAnnotation
persistent identity: annotation2317915
display id: annotation2317915
version: 1
top level: BBa_K092901
location: https://synbiohub.org/public/igem/BBa_K092901/annotation2317915/range2317915/1
component: BBa_K092900

https://synbiohub.org/public/igem/BBa_K092901/annotation2317915/range2317915/1
type: Range
persistent identity: range2317915
display id: range2317915
version: 1
top level: BBa_K092901
direction: direction/reverse
start: 1
end: 2102
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2317922
display id: annotation2317922
version: 1
top level: BBa_K092901
location: https://synbiohub.org/public/igem/BBa_K092901/annotation2317922/range2317922/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K092901/annotation2317922/range2317922/1
type: Range
persistent identity: range2317922
display id: range2317922
version: 1
top level: BBa_K092901
direction: direction/reverse
start: 2111
end: 2239
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2317923
display id: annotation2317923
version: 1
top level: BBa_K092901
location: https://synbiohub.org/public/igem/BBa_K092901/annotation2317923/range2317923/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K092901/annotation2317923/range2317923/1
type: Range
persistent identity: range2317923
display id: range2317923
version: 1
top level: BBa_K092901
direction: direction/forward
start: 2248
end: 2447
orientation: inline

BBa_K092700
type: SequenceAnnotation
persistent identity: annotation2317961
display id: annotation2317961
version: 1
top level: BBa_K092901
location: https://synbiohub.org/public/igem/BBa_K092901/annotation2317961/range2317961/1
component: BBa_K092700

https://synbiohub.org/public/igem/BBa_K092901/annotation2317961/range2317961/1
type: Range
persistent identity: range2317961
display id: range2317961
version: 1
top level: BBa_K092901
direction: direction/reverse
start: 2456
end: 4869
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2317939
display id: annotation2317939
version: 1
top level: BBa_K092901
location: https://synbiohub.org/public/igem/BBa_K092901/annotation2317939/range2317939/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K092901/annotation2317939/range2317939/1
type: Range
persistent identity: range2317939
display id: range2317939
version: 1
top level: BBa_K092901
direction: direction/reverse
start: 3167
end: 3295
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K092700
type: ComponentDefinition
persistent identity: BBa K092700
display id: BBa_K092700
version: 1
was generated by: iGEM to SBOL conversion
description: TetR cds with RBS and Terminator + pTetR, RFP with RBS + LuxI with RBS and terminator
created: 2008-10-18T11:00:00Z
modified: 2015-05-08T01:08:40Z
owned by: james
owned by: myers
mutable description: Combination of the following parts : BBa_K092000, BBa_K092300 and BBa_K092400: The TetR sequence (with a ribosome binding site and terminator)[BBa_K09200] is bound to the part containing the promoter of TetR and the red fluorescent protein (RFP) with a ribosome binding site. A luxI part (with RBS and terminator) is added at the end of the cds of the RFP. As no terminator was placed after the RFP, they both depends on the TetR promoter. The transcription of RFP and LuxI is inhibited when TetR is present. The production of TetR depends on the promoter you put in front of it.
discontinued: false
dominant: false
experience: experience/None
group u list: _193_
m user id: 0
owner id: 2618
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
star: true
creator: EPF-Lausanne Team 2008
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K092400
component: BBa_K092600
sequence annotation: BBa_K092600
sequence annotation: BBa_K092400
sequence: https://synbiohub.org/public/igem/BBa_K092700_sequence/1

BBa_K092400
type: Component
persistent identity: component2271680
display id: component2271680
version: 1
top level: BBa_K092700
definition: LuxI
access: public

BBa_K092600
type: Component
persistent identity: component2271675
display id: component2271675
version: 1
top level: BBa_K092700
definition: BBa_K092600
access: public

BBa_K092600
type: SequenceAnnotation
persistent identity: annotation2271675
display id: annotation2271675
version: 1
top level: BBa_K092700
location: https://synbiohub.org/public/igem/BBa_K092700/annotation2271675/range2271675/1
component: BBa_K092600

https://synbiohub.org/public/igem/BBa_K092700/annotation2271675/range2271675/1
type: Range
persistent identity: range2271675
display id: range2271675
version: 1
top level: BBa_K092700
direction: direction/reverse
start: 1
end: 1634
orientation: inline

BBa_K092400
type: SequenceAnnotation
persistent identity: annotation2271680
display id: annotation2271680
version: 1
top level: BBa_K092700
location: https://synbiohub.org/public/igem/BBa_K092700/annotation2271680/range2271680/1
component: BBa_K092400

https://synbiohub.org/public/igem/BBa_K092700/annotation2271680/range2271680/1
type: Range
persistent identity: range2271680
display id: range2271680
version: 1
top level: BBa_K092700
direction: direction/reverse
start: 1643
end: 2414
orientation: inline

BBa_K092900
type: ComponentDefinition
persistent identity: BBa K092900
display id: BBa_K092900
version: 1
was generated by: iGEM to SBOL conversion
description: RhlR controlled by a constitutive promoter, and LacI modified with a Rhl R promoter
created: 2008-10-21T11:00:00Z
modified: 2015-05-08T01:08:40Z
mutable provenance: The LacIm part comes from part BBa_K092800, from Basu et al."A synthetic multicelluar system for programmed pattern formation", Nature. 2005 Apr 28;434(7037):1130-4
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/None
group u list: _193_
m user id: 0
owner id: 2618
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
star: true
creator: EPF-Lausanne Team 2008
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_I14033
component: BBa_K092800
component: BBa_B0010
component: BBa_C0171
component: BBa_R0071
component: BBa_B0012
sequence annotation: BBa_I14033
sequence annotation: BBa_B0034
sequence annotation: BBa_C0171
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0071
sequence annotation: BBa_K092800
sequence: https://synbiohub.org/public/igem/BBa_K092900_sequence/1

BBa_B0034
type: Component
persistent identity: component1993094
display id: component1993094
version: 1
top level: BBa_K092900
definition: BBa_B0034
access: public

BBa_I14033
type: Component
persistent identity: component1993092
display id: component1993092
version: 1
top level: BBa_K092900
definition: Cat
access: public

BBa_K092800
type: Component
persistent identity: component1993108
display id: component1993108
version: 1
top level: BBa_K092900
definition: LacIm
access: public

BBa_B0010
type: Component
persistent identity: component1993096
display id: component1993096
version: 1
top level: BBa_K092900
definition: BBa_B0010
access: public

BBa_C0171
type: Component
persistent identity: component1993095
display id: component1993095
version: 1
top level: BBa_K092900
definition: rhIR
access: public

BBa_R0071
type: Component
persistent identity: component1993105
display id: component1993105
version: 1
top level: BBa_K092900
definition: RhlR+C4
access: public

BBa_B0012
type: Component
persistent identity: component1993098
display id: component1993098
version: 1
top level: BBa_K092900
definition: BBa_B0012
access: public

BBa_I14033
type: SequenceAnnotation
persistent identity: annotation1993092
display id: annotation1993092
version: 1
top level: BBa_K092900
location: https://synbiohub.org/public/igem/BBa_K092900/annotation1993092/range1993092/1
component: BBa_I14033

https://synbiohub.org/public/igem/BBa_K092900/annotation1993092/range1993092/1
type: Range
persistent identity: range1993092
display id: range1993092
version: 1
top level: BBa_K092900
direction: direction/reverse
start: 1
end: 38
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1993094
display id: annotation1993094
version: 1
top level: BBa_K092900
location: https://synbiohub.org/public/igem/BBa_K092900/annotation1993094/range1993094/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K092900/annotation1993094/range1993094/1
type: Range
persistent identity: range1993094
display id: range1993094
version: 1
top level: BBa_K092900
direction: direction/reverse
start: 47
end: 58
orientation: inline

BBa_C0171
type: SequenceAnnotation
persistent identity: annotation1993095
display id: annotation1993095
version: 1
top level: BBa_K092900
location: https://synbiohub.org/public/igem/BBa_K092900/annotation1993095/range1993095/1
component: BBa_C0171

https://synbiohub.org/public/igem/BBa_K092900/annotation1993095/range1993095/1
type: Range
persistent identity: range1993095
display id: range1993095
version: 1
top level: BBa_K092900
direction: direction/reverse
start: 65
end: 793
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1993096
display id: annotation1993096
version: 1
top level: BBa_K092900
location: https://synbiohub.org/public/igem/BBa_K092900/annotation1993096/range1993096/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K092900/annotation1993096/range1993096/1
type: Range
persistent identity: range1993096
display id: range1993096
version: 1
top level: BBa_K092900
direction: direction/forward
start: 802
end: 881
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1993098
display id: annotation1993098
version: 1
top level: BBa_K092900
location: https://synbiohub.org/public/igem/BBa_K092900/annotation1993098/range1993098/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K092900/annotation1993098/range1993098/1
type: Range
persistent identity: range1993098
display id: range1993098
version: 1
top level: BBa_K092900
direction: direction/forward
start: 890
end: 930
orientation: inline

BBa_R0071
type: SequenceAnnotation
persistent identity: annotation1993105
display id: annotation1993105
version: 1
top level: BBa_K092900
location: https://synbiohub.org/public/igem/BBa_K092900/annotation1993105/range1993105/1
component: BBa_R0071

https://synbiohub.org/public/igem/BBa_K092900/annotation1993105/range1993105/1
type: Range
persistent identity: range1993105
display id: range1993105
version: 1
top level: BBa_K092900
direction: direction/reverse
start: 939
end: 991
orientation: inline

BBa_K092800
type: SequenceAnnotation
persistent identity: annotation1993108
display id: annotation1993108
version: 1
top level: BBa_K092900
location: https://synbiohub.org/public/igem/BBa_K092900/annotation1993108/range1993108/1
component: BBa_K092800

https://synbiohub.org/public/igem/BBa_K092900/annotation1993108/range1993108/1
type: Range
persistent identity: range1993108
display id: range1993108
version: 1
top level: BBa_K092900
direction: direction/reverse
start: 1000
end: 2102
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

rhIR
type: ComponentDefinition
persistent identity: BBa C0171
display id: BBa_C0171
version: 1
was generated by: iGEM to SBOL conversion
description: rhlR repressor/activator from P. aeruginosa PA3477 (no LVA)
created: 2004-05-26T11:00:00Z
modified: 2015-08-31T04:07:24Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: same as C0071 except no LVA tag
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_C0171_sequence/1

RhlR+C4
type: ComponentDefinition
persistent identity: BBa R0071
display id: BBa_R0071
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (RhlR & C4-HSL regulated)
created: 2004-01-24T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: Pseudomonas aeruginosa rhlAB promoter
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Promoter activated by RhlR in concert with C4-HSL. C4-HSL is produced by RhlI, and acts as a quorum-sensing autoinducer. This is the natural sequence taken from Pseudomonas aeruginosa. Crosstalk: this promoter is also activated at a low level by LasR with its associated HSL.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The -10 and -35 sites are labeled as identified in [Pearson97]. The part begins with the RhlR binding site, and (rather arbitrarily) ends with the first transcribed base (+1).
star: true
creator: Ronny Krashinsky
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: RhlR
sequence annotation: -35
sequence annotation: -10
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0071_sequence/1

RhlR
type: SequenceAnnotation
persistent identity: annotation297102
display id: annotation297102
version: 1
top level: RhlR+C4
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297102/range297102/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0071/annotation297102/range297102/1
type: Range
persistent identity: range297102
display id: range297102
version: 1
top level: RhlR+C4
direction: direction/forward
start: 1
end: 20
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation297103
display id: annotation297103
version: 1
top level: RhlR+C4
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297103/range297103/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0071/annotation297103/range297103/1
type: Range
persistent identity: range297103
display id: range297103
version: 1
top level: RhlR+C4
direction: direction/forward
start: 19
end: 24
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation297104
display id: annotation297104
version: 1
top level: RhlR+C4
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297104/range297104/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0071/annotation297104/range297104/1
type: Range
persistent identity: range297104
display id: range297104
version: 1
top level: RhlR+C4
direction: direction/forward
start: 42
end: 47
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation297105
display id: annotation297105
version: 1
top level: RhlR+C4
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297105/range297105/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0071/annotation297105/range297105/1
type: Range
persistent identity: range297105
display id: range297105
version: 1
top level: RhlR+C4
direction: direction/forward
start: 53
end: 53
orientation: inline

Cat
type: ComponentDefinition
persistent identity: BBa I14033
display id: BBa_I14033
version: 1
was generated by: iGEM to SBOL conversion
description: P(Cat)
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:37Z
mutable provenance: Plasmid pACYC184
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Constitutive Promoter, Medium Transcription
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: P(Cat)
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_I14033_sequence/1

P(Cat)
type: SequenceAnnotation
persistent identity: annotation1026174
display id: annotation1026174
version: 1
top level: Cat
location: https://synbiohub.org/public/igem/BBa_I14033/annotation1026174/range1026174/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_I14033/annotation1026174/range1026174/1
type: Range
persistent identity: range1026174
display id: range1026174
version: 1
top level: Cat
direction: direction/forward
start: 1
end: 38
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1026172
display id: annotation1026172
version: 1
top level: Cat
location: https://synbiohub.org/public/igem/BBa_I14033/annotation1026172/range1026172/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_I14033/annotation1026172/range1026172/1
type: Range
persistent identity: range1026172
display id: range1026172
version: 1
top level: Cat
direction: direction/forward
start: 4
end: 9
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1026173
display id: annotation1026173
version: 1
top level: Cat
location: https://synbiohub.org/public/igem/BBa_I14033/annotation1026173/range1026173/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_I14033/annotation1026173/range1026173/1
type: Range
persistent identity: range1026173
display id: range1026173
version: 1
top level: Cat
direction: direction/forward
start: 27
end: 32
orientation: inline

LuxI
type: ComponentDefinition
persistent identity: BBa K092400
display id: BBa_K092400
version: 1
was generated by: iGEM to SBOL conversion
description: LuxI with RBS and Terminator
created: 2008-10-18T11:00:00Z
modified: 2015-05-08T01:08:39Z
owned by: james
owned by: myers
mutable description: LuxI with RBS and Terminator build by 2-steps PCR.
discontinued: false
dominant: false
experience: experience/None
group u list: _193_
m user id: 0
owner id: 2618
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
star: true
creator: EPF-Lausanne Team 2008
type: DnaRegion
role: partType/Signalling
role: SO:0000804
sequence annotation: B0034 RBS
sequence annotation: LuxI
sequence annotation: barcode
sequence annotation: B0015 Terminator
sequence: https://synbiohub.org/public/igem/BBa_K092400_sequence/1

B0034 RBS
type: SequenceAnnotation
persistent identity: annotation1982735
display id: annotation1982735
version: 1
top level: LuxI
location: https://synbiohub.org/public/igem/BBa_K092400/annotation1982735/range1982735/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K092400/annotation1982735/range1982735/1
type: Range
persistent identity: range1982735
display id: range1982735
version: 1
top level: LuxI
direction: direction/forward
start: 8
end: 19
orientation: inline

LuxI
type: SequenceAnnotation
persistent identity: annotation1982736
display id: annotation1982736
version: 1
top level: LuxI
location: https://synbiohub.org/public/igem/BBa_K092400/annotation1982736/range1982736/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K092400/annotation1982736/range1982736/1
type: Range
persistent identity: range1982736
display id: range1982736
version: 1
top level: LuxI
direction: direction/forward
start: 26
end: 607
orientation: inline

barcode
type: SequenceAnnotation
persistent identity: annotation1982738
display id: annotation1982738
version: 1
top level: LuxI
location: https://synbiohub.org/public/igem/BBa_K092400/annotation1982738/range1982738/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_K092400/annotation1982738/range1982738/1
type: Range
persistent identity: range1982738
display id: range1982738
version: 1
top level: LuxI
direction: direction/forward
start: 608
end: 643
orientation: inline

B0015 Terminator
type: SequenceAnnotation
persistent identity: annotation1982737
display id: annotation1982737
version: 1
top level: LuxI
location: https://synbiohub.org/public/igem/BBa_K092400/annotation1982737/range1982737/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K092400/annotation1982737/range1982737/1
type: Range
persistent identity: range1982737
display id: range1982737
version: 1
top level: LuxI
direction: direction/forward
start: 644
end: 772
orientation: inline

BBa_K092600
type: ComponentDefinition
persistent identity: BBa K092600
display id: BBa_K092600
version: 1
was generated by: iGEM to SBOL conversion
description: TetR cds with RBS and Terminator + pTetR, RFP with RBS
created: 2008-10-18T11:00:00Z
modified: 2015-05-08T01:08:39Z
owned by: james
owned by: myers
mutable description: Composite part : combination of the part BBa_K092000 and the BBa_K092300 : The TetR sequence (with a ribosome binding site and terminator) is bound to the part containing the promoter of TetR, a ribosome binding site and the red fluorescent protein (RFP). Thus, the production of RFP directly depends on the production of TetR. The transcription of RFP is inhibited when TetR is present. The production of TetR depends on the promoter you put in front of it.
discontinued: false
dominant: true
experience: experience/None
group u list: _193_
m user id: 0
owner id: 2618
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
star: true
creator: EPF-Lausanne Team 2008
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K092000
component: BBa_K092300
sequence annotation: BBa_K092000
sequence annotation: BBa_K092300
sequence: https://synbiohub.org/public/igem/BBa_K092600_sequence/1

BBa_K092000
type: Component
persistent identity: component2267078
display id: component2267078
version: 1
top level: BBa_K092600
definition: BBa_K092000
access: public

BBa_K092300
type: Component
persistent identity: component2267089
display id: component2267089
version: 1
top level: BBa_K092600
definition: BBa_K092300
access: public

BBa_K092000
type: SequenceAnnotation
persistent identity: annotation2267078
display id: annotation2267078
version: 1
top level: BBa_K092600
location: https://synbiohub.org/public/igem/BBa_K092600/annotation2267078/range2267078/1
component: BBa_K092000

https://synbiohub.org/public/igem/BBa_K092600/annotation2267078/range2267078/1
type: Range
persistent identity: range2267078
display id: range2267078
version: 1
top level: BBa_K092600
direction: direction/reverse
start: 1
end: 840
orientation: inline

BBa_K092300
type: SequenceAnnotation
persistent identity: annotation2267089
display id: annotation2267089
version: 1
top level: BBa_K092600
location: https://synbiohub.org/public/igem/BBa_K092600/annotation2267089/range2267089/1
component: BBa_K092300

https://synbiohub.org/public/igem/BBa_K092600/annotation2267089/range2267089/1
type: Range
persistent identity: range2267089
display id: range2267089
version: 1
top level: BBa_K092600
direction: direction/reverse
start: 849
end: 1634
orientation: inline

LacIm
type: ComponentDefinition
persistent identity: BBa K092800
display id: BBa_K092800
version: 1
was generated by: iGEM to SBOL conversion
description: Coding sequence for LacI modified with a different rate of translation than LacI, RBS
created: 2008-10-21T11:00:00Z
modified: 2015-05-08T01:08:40Z
mutable provenance: LacIm from Basu et al."A synthetic multicelluar system for programmed pattern formation", Nature. 2005 Apr 28;434(7037):1130-4
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/None
group u list: _193_
m user id: 0
owner id: 2618
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
star: true
creator: EPF-Lausanne Team 2008
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: B0034
sequence annotation: LacIm
sequence: https://synbiohub.org/public/igem/BBa_K092800_sequence/1

B0034
type: SequenceAnnotation
persistent identity: annotation1984080
display id: annotation1984080
version: 1
top level: LacIm
location: https://synbiohub.org/public/igem/BBa_K092800/annotation1984080/range1984080/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K092800/annotation1984080/range1984080/1
type: Range
persistent identity: range1984080
display id: range1984080
version: 1
top level: LacIm
direction: direction/forward
start: 1
end: 12
orientation: inline

LacIm
type: SequenceAnnotation
persistent identity: annotation1984081
display id: annotation1984081
version: 1
top level: LacIm
location: https://synbiohub.org/public/igem/BBa_K092800/annotation1984081/range1984081/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K092800/annotation1984081/range1984081/1
type: Range
persistent identity: range1984081
display id: range1984081
version: 1
top level: LacIm
direction: direction/forward
start: 21
end: 1103
orientation: inline

BBa_K092000
type: ComponentDefinition
persistent identity: BBa K092000
display id: BBa_K092000
version: 1
was generated by: iGEM to SBOL conversion
description: TetR coding sequence with RBS and terminator
created: 2008-10-11T11:00:00Z
modified: 2015-05-08T01:08:39Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding sequence of the Tetracycline repressor with the ribosome binding site and a double terminator. The TetR comes from transposon Tn10 and has a LVA tail (SsrA) [cf. C0040 part].
discontinued: false
dominant: false
experience: experience/None
group u list: _193_
m user id: 0
owner id: 2618
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: EPF-Lausanne Team 2008
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0034
component: BBa_B0015
component: BBa_C0040
sequence annotation: BBa_B0034
sequence annotation: BBa_C0040
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K092000_sequence/1

BBa_B0034
type: Component
persistent identity: component2249199
display id: component2249199
version: 1
top level: BBa_K092000
definition: BBa_B0034
access: public

BBa_B0015
type: Component
persistent identity: component2249210
display id: component2249210
version: 1
top level: BBa_K092000
definition: BBa_B0015
access: public

BBa_C0040
type: Component
persistent identity: component2249203
display id: component2249203
version: 1
top level: BBa_K092000
definition: tetR
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2249199
display id: annotation2249199
version: 1
top level: BBa_K092000
location: https://synbiohub.org/public/igem/BBa_K092000/annotation2249199/range2249199/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K092000/annotation2249199/range2249199/1
type: Range
persistent identity: range2249199
display id: range2249199
version: 1
top level: BBa_K092000
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2249203
display id: annotation2249203
version: 1
top level: BBa_K092000
location: https://synbiohub.org/public/igem/BBa_K092000/annotation2249203/range2249203/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K092000/annotation2249203/range2249203/1
type: Range
persistent identity: range2249203
display id: range2249203
version: 1
top level: BBa_K092000
direction: direction/reverse
start: 19
end: 703
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2249210
display id: annotation2249210
version: 1
top level: BBa_K092000
location: https://synbiohub.org/public/igem/BBa_K092000/annotation2249210/range2249210/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K092000/annotation2249210/range2249210/1
type: Range
persistent identity: range2249210
display id: range2249210
version: 1
top level: BBa_K092000
direction: direction/reverse
start: 712
end: 840
orientation: inline

BBa_K092300
type: ComponentDefinition
persistent identity: BBa K092300
display id: BBa_K092300
version: 1
was generated by: iGEM to SBOL conversion
description: RFP controlled by a TetR promoter
created: 2008-10-12T11:00:00Z
modified: 2015-05-08T01:08:39Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding sequence of the RFP with a ribosome binding site and pTetR as promoter. Thus, the transcription of RFP is inhibited when TetR is present.
discontinued: false
dominant: false
experience: experience/None
group u list: _193_
m user id: 0
owner id: 2618
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: EPF-Lausanne Team 2008
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_R0040
component: BBa_E1010
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_K092300_sequence/1

BBa_B0034
type: Component
persistent identity: component2246109
display id: component2246109
version: 1
top level: BBa_K092300
definition: BBa_B0034
access: public

BBa_R0040
type: Component
persistent identity: component2246103
display id: component2246103
version: 1
top level: BBa_K092300
definition: p(tetR)
access: public

BBa_E1010
type: Component
persistent identity: component2246112
display id: component2246112
version: 1
top level: BBa_K092300
definition: mRFP1
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2246103
display id: annotation2246103
version: 1
top level: BBa_K092300
location: https://synbiohub.org/public/igem/BBa_K092300/annotation2246103/range2246103/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K092300/annotation2246103/range2246103/1
type: Range
persistent identity: range2246103
display id: range2246103
version: 1
top level: BBa_K092300
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2246109
display id: annotation2246109
version: 1
top level: BBa_K092300
location: https://synbiohub.org/public/igem/BBa_K092300/annotation2246109/range2246109/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K092300/annotation2246109/range2246109/1
type: Range
persistent identity: range2246109
display id: range2246109
version: 1
top level: BBa_K092300
direction: direction/reverse
start: 63
end: 74
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2246112
display id: annotation2246112
version: 1
top level: BBa_K092300
location: https://synbiohub.org/public/igem/BBa_K092300/annotation2246112/range2246112/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K092300/annotation2246112/range2246112/1
type: Range
persistent identity: range2246112
display id: range2246112
version: 1
top level: BBa_K092300
direction: direction/reverse
start: 81
end: 786
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa C0040
display id: BBa_C0040
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline repressor from transposon Tn10 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: tetR
sequence annotation: SsrA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0040_sequence/1

tetR
type: SequenceAnnotation
persistent identity: annotation23329
display id: annotation23329
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
type: Range
persistent identity: range23329
display id: range23329
version: 1
top level: tetR
direction: direction/forward
start: 4
end: 620
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation23330
display id: annotation23330
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
type: Range
persistent identity: range23330
display id: range23330
version: 1
top level: tetR
direction: direction/forward
start: 621
end: 654
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213989
display id: annotation2213989
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
type: Range
persistent identity: range2213989
display id: range2213989
version: 1
top level: tetR
direction: direction/forward
start: 661
end: 685
orientation: inline

https://synbiohub.org/public/igem/BBa_K092901_sequence/1
type: Sequence
persistent identity: BBa K092901 sequence
display id: BBa_K092901_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggcacgtaagaggttccaactttcaccataatgaaacatactagagaaagaggagaaatactagatgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcctgtgaaatctggcagttaccgttagctttcgaattggctaaaaagtgttctactagagaaagaggagaaatactagagtcattggccagactctaagcggctcacttggcgcgctaattgcatcaggctgtccgccagggcacgcgggctcgccgtctgggtgttcggggctaacgtcgtcttgcgcttaactaagctcaccggcagtaattggttacctttaacggcttgaccttggctcagctgtaacagacgatcaacagacgtctggcctaataaacggaagtcttgcttaatcgtcgtgcttggtggaatgtagcagctagaatcctccgtgtcatcatagccaaccacgctaatgtcggcgcccacacgtaagccgctttccgtgatcgcacgcatcgcacctaaggccatttggtcattcgccactaacatggcggtcggcacaataccttcgtttaacatctgcatcgtctgctggaagccgctcatcgcgctccaatcaccctcgcgctcggcaattggttggatctggttacgggtcaggtacttgtgccaacccgctaagcgtaagcgggcgctcacgctgctcagcggaccggccagtaaggcaatctgttgatggcccagcgccactaagtgttcaacacctaagcgggtgccatcctcgtggctaaagatgatgctattaatcggggtttgatcgctcacgtccaggaacagggctggcacgttggtacacgccgcctcaaccgcgatcgcgtcttgatcgtctaatgggtaattgataattaagccgctcacacgctgggccagcaggttatgaacggcggccttgcacgcctccacaccagagcgctccaccatgctaacaacaacagacgcgcctaactggtccgcacggctcttgatggcggccacgatctggcttggggcatgtaacgctaagctgctcgtcgccacaccgattaacaggctttgcttaccggctaactgctgcgcaacacgattcgggatatagtttaattcggccatggcggcctcaaccttctcgcg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K092700_sequence/1
type: Sequence
persistent identity: BBa K092700 sequence
display id: BBa_K092700_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagcttgcataaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K092900_sequence/1
type: Sequence
persistent identity: BBa K092900 sequence
display id: BBa_K092900_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggcacgtaagaggttccaactttcaccataatgaaacatactagagaaagaggagaaatactagatgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcctgtgaaatctggcagttaccgttagctttcgaattggctaaaaagtgttctactagagaaagaggagaaatactagagtcattggccagactctaagcggctcacttggcgcgctaattgcatcaggctgtccgccagggcacgcgggctcgccgtctgggtgttcggggctaacgtcgtcttgcgcttaactaagctcaccggcagtaattggttacctttaacggcttgaccttggctcagctgtaacagacgatcaacagacgtctggcctaataaacggaagtcttgcttaatcgtcgtgcttggtggaatgtagcagctagaatcctccgtgtcatcatagccaaccacgctaatgtcggcgcccacacgtaagccgctttccgtgatcgcacgcatcgcacctaaggccatttggtcattcgccactaacatggcggtcggcacaataccttcgtttaacatctgcatcgtctgctggaagccgctcatcgcgctccaatcaccctcgcgctcggcaattggttggatctggttacgggtcaggtacttgtgccaacccgctaagcgtaagcgggcgctcacgctgctcagcggaccggccagtaaggcaatctgttgatggcccagcgccactaagtgttcaacacctaagcgggtgccatcctcgtggctaaagatgatgctattaatcggggtttgatcgctcacgtccaggaacagggctggcacgttggtacacgccgcctcaaccgcgatcgcgtcttgatcgtctaatgggtaattgataattaagccgctcacacgctgggccagcaggttatgaacggcggccttgcacgcctccacaccagagcgctccaccatgctaacaacaacagacgcgcctaactggtccgcacggctcttgatggcggccacgatctggcttggggcatgtaacgctaagctgctcgtcgccacaccgattaacaggctttgcttaccggctaactgctgcgcaacacgattcgggatatagtttaattcggccatggcggcctcaaccttctcgcg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0171_sequence/1
type: Sequence
persistent identity: BBa C0171 sequence
display id: BBa_C0171_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0071_sequence/1
type: Sequence
persistent identity: BBa R0071 sequence
display id: BBa_R0071_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcctgtgaaatctggcagttaccgttagctttcgaattggctaaaaagtgttc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I14033_sequence/1
type: Sequence
persistent identity: BBa I14033 sequence
display id: BBa_I14033_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggcacgtaagaggttccaactttcaccataatgaaaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K092400_sequence/1
type: Sequence
persistent identity: BBa K092400 sequence
display id: BBa_K092400_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cttgcataaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaattaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K092600_sequence/1
type: Sequence
persistent identity: BBa K092600 sequence
display id: BBa_K092600_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K092800_sequence/1
type: Sequence
persistent identity: BBa K092800 sequence
display id: BBa_K092800_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagagtcattggccagactctaagcggctcacttggcgcgctaattgcatcaggctgtccgccagggcacgcgggctcgccgtctgggtgttcggggctaacgtcgtcttgcgcttaactaagctcaccggcagtaattggttacctttaacggcttgaccttggctcagctgtaacagacgatcaacagacgtctggcctaataaacggaagtcttgcttaatcgtcgtgcttggtggaatgtagcagctagaatcctccgtgtcatcatagccaaccacgctaatgtcggcgcccacacgtaagccgctttccgtgatcgcacgcatcgcacctaaggccatttggtcattcgccactaacatggcggtcggcacaataccttcgtttaacatctgcatcgtctgctggaagccgctcatcgcgctccaatcaccctcgcgctcggcaattggttggatctggttacgggtcaggtacttgtgccaacccgctaagcgtaagcgggcgctcacgctgctcagcggaccggccagtaaggcaatctgttgatggcccagcgccactaagtgttcaacacctaagcgggtgccatcctcgtggctaaagatgatgctattaatcggggtttgatcgctcacgtccaggaacagggctggcacgttggtacacgccgcctcaaccgcgatcgcgtcttgatcgtctaatgggtaattgataattaagccgctcacacgctgggccagcaggttatgaacggcggccttgcacgcctccacaccagagcgctccaccatgctaacaacaacagacgcgcctaactggtccgcacggctcttgatggcggccacgatctggcttggggcatgtaacgctaagctgctcgtcgccacaccgattaacaggctttgcttaccggctaactgctgcgcaacacgattcgggatatagtttaattcggccatggcggcctcaaccttctcgcgggtcttggcgctcacatgagacgcttgattaacaacacggctcaccgtttggtagctcacgcccgcgtattccgccacgtcatacagggtcaccggcttcat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K092000_sequence/1
type: Sequence
persistent identity: BBa K092000 sequence
display id: BBa_K092000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K092300_sequence/1
type: Sequence
persistent identity: BBa K092300 sequence
display id: BBa_K092300_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0040_sequence/1
type: Sequence
persistent identity: BBa C0040 sequence
display id: BBa_C0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z