BBa_K093000
1
pRecA
pRecA with LexA binding site
2008-10-25T11:00:00Z
2015-05-08T01:08:40Z
synthetic. Oligonucleotides were designed to form the double stranded molecule with sticky ends for direct ligation into a biobrick vector.
This is a pRecA promoter with LexA binding sites. LexA is cleaved by RecA when RecA is upregulated due to induction of the SOS response triggered by single stranded DNA. For this part to work it must be in a RecA+ strain of E. coli such as TG1
false
false
_247_
0
3630
9
It's complicated
false
How far past the start site of transcription do you need to go in order for the promoter to drive transcription? The promoter has been confirmed to drive transcription in a regular DH5alpha strain, so it the answer to the question is, 3 bp before the suffix, maybe less.
The LexA binding site is in the core region of the promoter (between the -35 and -10 sigma 70 binding sites). According to Robert Sidney Cox III and colleagues in their paper "Programming ene expression with combinatorial promoters, the core region is the most effective region for binding of repressor molecules.
false
Julian Wiegelmann, Danielle Nash
annotation1989775
1
-10
range1989775
1
30
39
annotation1989779
1
LexA binding
range1989779
1
15
35
annotation1989749
1
-35
range1989749
1
11
17
BBa_K093000_sequence
1
tacaaaacacttgatactgtatatatatacagtataattgcttcaaca
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z