BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_K093004
1
rPlac+TT
Convergent Promoter System: Reverse Module: rPlac+TT
2008-10-27T12:00:00Z
2015-05-08T01:08:40Z
Synthetic
This part contains a reverse BBa_R0011 followed by a forward TT, BBa_B0015.
false
false
_247_
0
3630
9
It's complicated
false
The forward and reverse efficiencies of TTs in the registry had to be considered, especially for this part's convergent partner, BBa_K093003. R0011 was used instead of R0010 to avoid catabolite repression.
The idea is to place BBa_K093003 upstream of the gene of interest, that requires tight repression, and to place BBa_K093004 downstream. If the cells are grown in the presence of IPTG POlacI is induced and anti-sense mRNA is produced. Anti-sense mRNA will repress expression of the gene of interest through RNAi. This system also requires cI repressor (BBa_C0051) under control of POlacI (BBa_R0011). This system would be functional in a laqIq strain such as E. coli. TG1.
O'Connor,C.D., & Timmins, K.N. (1987). Highly repressible expression system for cloning genes that specify potentialy toxic proteins. Journal of Bacteriology. 169, 4457-4462.
false
John Heil, Danielle Nash, Shira Davis
component1996703
1
BBa_B0012
component1996701
1
BBa_B0010
component1996700
1
BBa_K093008
annotation1996700
1
BBa_K093008
range1996700
1
1
55
annotation1996701
1
BBa_B0010
range1996701
1
64
143
annotation1996703
1
BBa_B0012
range1996703
1
152
192
BBa_K093008
1
BBa_K093008
reverse BBa_R0011
2008-10-27T12:00:00Z
2015-05-08T01:08:40Z
synthetic
reverse compliments of BBa_R0011
false
false
_247_
0
3630
9
Not in stock
false
for downstream application
false
John Heil
annotation1992541
1
-35
range1992541
1
30
35
annotation1992540
1
-10
range1992540
1
8
14
annotation1992543
1
lacO1
range1992543
1
15
29
annotation1992542
1
lacO1
range1992542
1
36
52
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K093008_sequence
1
tgtgctcagtatcttgttatccgctcacaatgtcaattgttatccgctcacaatt
BBa_K093004_sequence
1
tgtgctcagtatcttgttatccgctcacaatgtcaattgttatccgctcacaatttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z