BBa_K093002 1 PmeIR PmeI endonuclease 2008-10-25T11:00:00Z 2015-05-08T01:08:40Z Synthetic from GENEART This is the PmeI ORF, coding for the PmeI restriction endonuclease gene with the recognition sequence: GTTTAAAC false false _247_ 0 3630 9 It's complicated false The stop codon was changed to TAA. false John Heil BBa_K093001 1 T7Gene6 T7 Gene 6 exonuclease 2008-10-25T11:00:00Z 2015-05-08T01:08:40Z T7 genomic DNA This is the T7 gene 6 ORF, that codes for the T7 gene 6 5'-3' exonuclease. For properties of T7 gene 6 exonuclease see: Kerr and Sadowski "Gene 6 Exonuclease of Bacteriophage T7" The Journal of Biological Chemistry Vol. 247 No. 1 January 10, pp 311-318, 1972. false false _247_ 0 3630 9 It's complicated false We needed to change the stop codon to the standard stop codon TAA. We later realized that we should have added tandem TAA. The Tandem TAA stop codon is present in the composite parts containing this ORF. The ORF alone has the single TAA stop codon. false John Heil, Shira Davis, Danielle Nash BBa_K093014 1 BBa_K093014 Nuclease construct 2008-11-17T12:00:00Z 2015-05-08T01:08:40Z T7 phage, synthetic T7 gene6 and pmeI false false _247_ 0 3627 9 Not in stock false Order of nucleases false Erica Tiberia component1999495 1 BBa_K093002 component1999494 1 BBa_K093001 annotation1999494 1 BBa_K093001 range1999494 1 1 903 annotation1999495 1 BBa_K093002 range1999495 1 910 1602 BBa_K093014_sequence 1 atggcacttcttgaccttaaacaattctatgagttacgtgaaggctgcgacgacaagggtatccttgtgatggacggcgactggctggtcttccaagctatgagtgctgctgagtttgatgcctcttgggaggaagagatttggcaccgatgctgtgaccacgctaaggcccgtcagattcttgaggattccattaagtcctacgagacccgtaagaaggcttgggcaggtgctccaattgtccttgcgttcaccgatagtgttaactggcgtaaagaactggttgacccgaactataaggctaaccgtaaggccgtgaagaaacctgtagggtactttgagttccttgatgctctctttgagcgcgaagagttctattgcatccgtgagcctatgcttgagggtgatgacgttatgggagttattgcttccaatccgtctgccttcggtgctcgtaaggctgtaatcatctcttgcgataaggactttaagaccatccctaactgtgacttcctgtggtgtaccactggtaacatcctgactcagaccgaagagtccgctgactggtggcacctcttccagaccatcaagggtgacatcactgatggttactcagggattgctggatggggtgataccgccgaggacttcttgaataacccgttcataaccgagcctaaaacgtctgtgcttaagtccggtaagaacaaaggccaagaggttactaaatgggttaaacgcgaccctgagcctcatgagacgctttgggactgcattaagtccattggcgcgaaggctggtatgaccgaagaggatattatcaagcagggccaaatggctcgaatcctacggttcaacgagtacaactttattgacaaggagatttacctgtggagaccgtaatactagatgacaacaaactccccctcagacgtcggcatgatcgacgagtgtctgtccatcgtccgaacgtcgcttgcacgatgtttccaacagcaggccccaagcattcaagcctcatggccactttcaggacgcgccgtatctgagattggaggccgcctagtcgagagtttcgttttagcacgactcccgcatgaactgagcaccacgccttttgacggccagattctatgtgaaatacctgaatccggcagagcgatggaagacattgcggtgaccttcatcggcccacatggaagggctcgactactcatcgacgtcaagggtcataacgaataccgcacgggatcgagacccaatttggcttcgatccgaaaatgtctggaactctatcgcagctcctcacataccgttgatgagctcgttgtcttcttctgccgttaccgcccatccgtccacccggatcatcacgcacaagcggtcgaatatcacgttctgcccgagtcgttaatgagcagggattttcctgcttcgtgccctgagcgaaagcaacctggatccagccaatatcggagtggcggccagttgctgcttgccagggaaaacaacatacggttagtgaatcgttcaaggtcggagttcgttcaacttctggagggtctccagtcacgccttcaacgggggcgaagtacggtttaa BBa_K093001_sequence 1 atggcacttcttgaccttaaacaattctatgagttacgtgaaggctgcgacgacaagggtatccttgtgatggacggcgactggctggtcttccaagctatgagtgctgctgagtttgatgcctcttgggaggaagagatttggcaccgatgctgtgaccacgctaaggcccgtcagattcttgaggattccattaagtcctacgagacccgtaagaaggcttgggcaggtgctccaattgtccttgcgttcaccgatagtgttaactggcgtaaagaactggttgacccgaactataaggctaaccgtaaggccgtgaagaaacctgtagggtactttgagttccttgatgctctctttgagcgcgaagagttctattgcatccgtgagcctatgcttgagggtgatgacgttatgggagttattgcttccaatccgtctgccttcggtgctcgtaaggctgtaatcatctcttgcgataaggactttaagaccatccctaactgtgacttcctgtggtgtaccactggtaacatcctgactcagaccgaagagtccgctgactggtggcacctcttccagaccatcaagggtgacatcactgatggttactcagggattgctggatggggtgataccgccgaggacttcttgaataacccgttcataaccgagcctaaaacgtctgtgcttaagtccggtaagaacaaaggccaagaggttactaaatgggttaaacgcgaccctgagcctcatgagacgctttgggactgcattaagtccattggcgcgaaggctggtatgaccgaagaggatattatcaagcagggccaaatggctcgaatcctacggttcaacgagtacaactttattgacaaggagatttacctgtggagaccgtaa BBa_K093002_sequence 1 atgacaacaaactccccctcagacgtcggcatgatcgacgagtgtctgtccatcgtccgaacgtcgcttgcacgatgtttccaacagcaggccccaagcattcaagcctcatggccactttcaggacgcgccgtatctgagattggaggccgcctagtcgagagtttcgttttagcacgactcccgcatgaactgagcaccacgccttttgacggccagattctatgtgaaatacctgaatccggcagagcgatggaagacattgcggtgaccttcatcggcccacatggaagggctcgactactcatcgacgtcaagggtcataacgaataccgcacgggatcgagacccaatttggcttcgatccgaaaatgtctggaactctatcgcagctcctcacataccgttgatgagctcgttgtcttcttctgccgttaccgcccatccgtccacccggatcatcacgcacaagcggtcgaatatcacgttctgcccgagtcgttaatgagcagggattttcctgcttcgtgccctgagcgaaagcaacctggatccagccaatatcggagtggcggccagttgctgcttgccagggaaaacaacatacggttagtgaatcgttcaaggtcggagttcgttcaacttctggagggtctccagtcacgccttcaacgggggcgaagtacggtttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z